Experiment: 2-Furfuraldehyde 0.125 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hofC and hofB overlap by 11 nucleotides hofB and ppdD are separated by 9 nucleotides ppdD and nadC are separated by 202 nucleotides
b0106: hofC - assembly protein in type IV pilin biogenesis, transmembrane protein (NCBI), at 114,522 to 115,724
hofC
b0107: hofB - conserved protein with nucleoside triphosphate hydrolase domain (NCBI), at 115,714 to 117,099
hofB
b0108: ppdD - predicted major pilin subunit (NCBI), at 117,109 to 117,549
ppdD
b0109: nadC - nicotinate-nucleotide pyrophosphorylase (NCBI), at 117,752 to 118,645
nadC
Position (kb)
115
116
117
118 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 114.838 kb on + strand, within hofC at 114.838 kb on + strand, within hofC at 114.844 kb on + strand, within hofC at 114.928 kb on - strand, within hofC at 114.930 kb on - strand at 114.930 kb on - strand, within hofC at 114.998 kb on + strand, within hofC at 114.998 kb on + strand, within hofC at 115.010 kb on - strand, within hofC at 115.021 kb on + strand, within hofC at 115.021 kb on + strand, within hofC at 115.077 kb on - strand, within hofC at 115.152 kb on - strand, within hofC at 115.152 kb on - strand, within hofC at 115.201 kb on - strand, within hofC at 115.203 kb on - strand, within hofC at 115.203 kb on - strand, within hofC at 115.273 kb on + strand, within hofC at 115.278 kb on + strand, within hofC at 115.278 kb on + strand, within hofC at 115.330 kb on + strand, within hofC at 115.367 kb on + strand, within hofC at 115.367 kb on + strand, within hofC at 115.379 kb on + strand, within hofC at 115.379 kb on + strand, within hofC at 115.409 kb on + strand, within hofC at 115.474 kb on - strand, within hofC at 115.507 kb on + strand, within hofC at 115.507 kb on + strand, within hofC at 115.515 kb on - strand, within hofC at 115.624 kb on + strand at 115.651 kb on + strand at 115.699 kb on - strand at 115.737 kb on + strand at 115.856 kb on - strand, within hofB at 115.856 kb on - strand, within hofB at 115.910 kb on + strand, within hofB at 115.911 kb on - strand, within hofB at 115.911 kb on - strand, within hofB at 115.941 kb on + strand, within hofB at 115.980 kb on + strand, within hofB at 116.058 kb on + strand, within hofB at 116.149 kb on - strand, within hofB at 116.335 kb on + strand, within hofB at 116.399 kb on + strand, within hofB at 116.430 kb on - strand, within hofB at 116.430 kb on - strand, within hofB at 116.471 kb on - strand, within hofB at 116.760 kb on - strand, within hofB at 116.777 kb on + strand, within hofB at 116.777 kb on + strand, within hofB at 116.872 kb on + strand, within hofB at 116.882 kb on + strand, within hofB at 116.957 kb on - strand, within hofB at 116.964 kb on - strand at 117.046 kb on + strand at 117.056 kb on + strand at 117.069 kb on + strand at 117.075 kb on + strand at 117.075 kb on + strand at 117.075 kb on + strand at 117.081 kb on - strand at 117.081 kb on - strand at 117.084 kb on + strand at 117.087 kb on + strand at 117.227 kb on - strand, within ppdD at 117.281 kb on + strand, within ppdD at 117.281 kb on + strand, within ppdD at 117.293 kb on - strand, within ppdD at 117.302 kb on + strand, within ppdD at 117.302 kb on + strand, within ppdD at 117.385 kb on + strand, within ppdD at 117.387 kb on + strand, within ppdD at 117.508 kb on - strand at 117.508 kb on - strand at 117.511 kb on - strand at 117.511 kb on - strand at 117.525 kb on - strand at 117.525 kb on - strand at 117.603 kb on + strand at 117.603 kb on + strand at 117.839 kb on + strand at 117.856 kb on - strand, within nadC at 117.856 kb on - strand, within nadC at 117.879 kb on - strand, within nadC at 117.879 kb on - strand, within nadC at 117.881 kb on - strand, within nadC at 117.922 kb on - strand, within nadC at 117.922 kb on - strand, within nadC
Per-strain Table
Position Strand Gene LocusTag Fraction 2-Furfuraldehyde 0.125 vol% remove 114,838 + hofC b0106 0.26 +1.2 114,838 + hofC b0106 0.26 +2.1 114,844 + hofC b0106 0.27 -0.7 114,928 - hofC b0106 0.34 -0.3 114,930 - -0.0 114,930 - hofC b0106 0.34 -0.3 114,998 + hofC b0106 0.40 +1.2 114,998 + hofC b0106 0.40 -0.4 115,010 - hofC b0106 0.41 +0.4 115,021 + hofC b0106 0.41 +0.7 115,021 + hofC b0106 0.41 -0.2 115,077 - hofC b0106 0.46 +0.3 115,152 - hofC b0106 0.52 -0.1 115,152 - hofC b0106 0.52 -0.0 115,201 - hofC b0106 0.56 -0.5 115,203 - hofC b0106 0.57 +1.8 115,203 - hofC b0106 0.57 +0.6 115,273 + hofC b0106 0.62 -0.5 115,278 + hofC b0106 0.63 -0.4 115,278 + hofC b0106 0.63 -0.2 115,330 + hofC b0106 0.67 +0.8 115,367 + hofC b0106 0.70 -0.8 115,367 + hofC b0106 0.70 +0.2 115,379 + hofC b0106 0.71 -1.2 115,379 + hofC b0106 0.71 -0.6 115,409 + hofC b0106 0.74 -1.0 115,474 - hofC b0106 0.79 -0.0 115,507 + hofC b0106 0.82 -0.6 115,507 + hofC b0106 0.82 -1.0 115,515 - hofC b0106 0.83 -0.4 115,624 + +0.5 115,651 + -0.0 115,699 - -1.3 115,737 + -0.7 115,856 - hofB b0107 0.10 -0.0 115,856 - hofB b0107 0.10 -0.4 115,910 + hofB b0107 0.14 -0.1 115,911 - hofB b0107 0.14 -0.4 115,911 - hofB b0107 0.14 -1.2 115,941 + hofB b0107 0.16 -1.1 115,980 + hofB b0107 0.19 +0.4 116,058 + hofB b0107 0.25 -0.2 116,149 - hofB b0107 0.31 -0.0 116,335 + hofB b0107 0.45 -0.1 116,399 + hofB b0107 0.49 -1.2 116,430 - hofB b0107 0.52 +0.1 116,430 - hofB b0107 0.52 -0.3 116,471 - hofB b0107 0.55 -0.7 116,760 - hofB b0107 0.75 +0.0 116,777 + hofB b0107 0.77 -0.0 116,777 + hofB b0107 0.77 +0.6 116,872 + hofB b0107 0.84 -0.8 116,882 + hofB b0107 0.84 -2.5 116,957 - hofB b0107 0.90 -0.3 116,964 - -0.3 117,046 + +0.5 117,056 + +0.3 117,069 + +0.3 117,075 + -1.0 117,075 + +0.2 117,075 + -0.3 117,081 - +0.5 117,081 - -0.6 117,084 + +0.6 117,087 + +0.0 117,227 - ppdD b0108 0.27 -0.2 117,281 + ppdD b0108 0.39 -0.6 117,281 + ppdD b0108 0.39 -1.0 117,293 - ppdD b0108 0.42 -0.1 117,302 + ppdD b0108 0.44 +0.7 117,302 + ppdD b0108 0.44 +0.6 117,385 + ppdD b0108 0.63 +0.1 117,387 + ppdD b0108 0.63 -0.2 117,508 - -0.0 117,508 - +2.0 117,511 - +0.4 117,511 - -0.6 117,525 - -0.0 117,525 - -0.0 117,603 + -0.3 117,603 + -1.5 117,839 + +1.1 117,856 - nadC b0109 0.12 +2.0 117,856 - nadC b0109 0.12 -1.0 117,879 - nadC b0109 0.14 -2.0 117,879 - nadC b0109 0.14 +0.6 117,881 - nadC b0109 0.14 +0.2 117,922 - nadC b0109 0.19 -0.9 117,922 - nadC b0109 0.19 -0.6
Or see this region's nucleotide sequence