Strain Fitness in Escherichia coli BW25113 around b3805

Experiment: methylglyoxal 0.008 vol%

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nthemX and hemD are separated by 21 nucleotideshemD and hemC overlap by 4 nucleotideshemC and cyaA are separated by 386 nucleotides b3803: hemX - predicted uroporphyrinogen III methylase (NCBI), at 3,985,908 to 3,987,089 hemX b3804: hemD - uroporphyrinogen-III synthetase (NCBI), at 3,987,111 to 3,987,851 hemD b3805: hemC - porphobilinogen deaminase (NCBI), at 3,987,848 to 3,988,789 hemC b3806: cyaA - adenylate cyclase (NCBI), at 3,989,176 to 3,991,722 cyaA Position (kb) 3987 3988 3989Strain fitness (log2 ratio) -1 0 1at 3986.897 kb on + strand, within hemXat 3986.897 kb on + strand, within hemXat 3986.922 kb on + strand, within hemXat 3986.948 kb on + strand, within hemXat 3986.964 kb on - strand, within hemXat 3986.964 kb on - strand, within hemXat 3987.023 kb on + strandat 3987.023 kb on + strandat 3987.027 kb on - strandat 3987.027 kb on - strandat 3987.031 kb on + strandat 3987.033 kb on + strandat 3987.048 kb on + strandat 3987.048 kb on - strandat 3987.051 kb on - strandat 3987.059 kb on - strandat 3987.101 kb on + strandat 3987.114 kb on + strandat 3988.789 kb on - strandat 3988.800 kb on - strandat 3988.800 kb on - strandat 3988.830 kb on - strandat 3988.830 kb on - strandat 3988.901 kb on - strandat 3988.901 kb on - strandat 3988.917 kb on + strandat 3988.917 kb on + strandat 3988.958 kb on + strandat 3988.961 kb on + strandat 3988.966 kb on + strandat 3988.983 kb on + strandat 3989.014 kb on - strandat 3989.079 kb on - strandat 3989.173 kb on + strandat 3989.173 kb on + strandat 3989.181 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction methylglyoxal 0.008 vol%
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3,986,897 + hemX b3803 0.84 +0.4
3,986,897 + hemX b3803 0.84 +0.7
3,986,922 + hemX b3803 0.86 +1.0
3,986,948 + hemX b3803 0.88 -0.3
3,986,964 - hemX b3803 0.89 +0.4
3,986,964 - hemX b3803 0.89 +0.5
3,987,023 + -0.6
3,987,023 + -0.3
3,987,027 - -0.2
3,987,027 - -0.5
3,987,031 + -0.6
3,987,033 + +0.1
3,987,048 + +0.0
3,987,048 - -0.1
3,987,051 - -0.0
3,987,059 - -1.3
3,987,101 + -0.0
3,987,114 + -1.4
3,988,789 - -1.3
3,988,800 - +0.2
3,988,800 - +0.3
3,988,830 - +0.8
3,988,830 - -0.7
3,988,901 - +0.8
3,988,901 - +0.1
3,988,917 + -1.3
3,988,917 + -0.7
3,988,958 + -0.5
3,988,961 + +0.3
3,988,966 + +0.8
3,988,983 + -0.0
3,989,014 - +1.3
3,989,079 - +0.7
3,989,173 + -0.1
3,989,173 + +0.1
3,989,181 + +0.3

Or see this region's nucleotide sequence