Strain Fitness in Escherichia coli BW25113 around b4179

Experiment: methylglyoxal 0.008 vol%

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntpurA and yjeB are separated by 204 nucleotidesyjeB and vacB are separated by 38 nucleotidesvacB and rlmB are separated by 179 nucleotides b4177: purA - adenylosuccinate synthetase (NCBI), at 4,402,710 to 4,404,008 purA b4178: yjeB - predicted DNA-binding transcriptional regulator (NCBI), at 4,404,213 to 4,404,638 yjeB b4179: vacB - putative enzyme (VIMSS), at 4,404,677 to 4,407,118 vacB b4180: rlmB - 23S rRNA (Gm2251)-methyltransferase (NCBI), at 4,407,298 to 4,408,029 rlmB Position (kb) 4404 4405 4406 4407 4408Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 4403.855 kb on - strand, within purAat 4403.965 kb on + strandat 4403.967 kb on - strandat 4404.050 kb on + strandat 4404.050 kb on + strandat 4404.050 kb on + strandat 4404.050 kb on + strandat 4404.070 kb on - strandat 4404.095 kb on - strandat 4404.095 kb on - strandat 4404.097 kb on + strandat 4404.097 kb on + strandat 4404.169 kb on - strandat 4404.254 kb on + strandat 4404.311 kb on + strand, within yjeBat 4404.311 kb on + strand, within yjeBat 4404.312 kb on + strand, within yjeBat 4404.312 kb on + strand, within yjeBat 4404.364 kb on - strand, within yjeBat 4404.364 kb on - strand, within yjeBat 4404.379 kb on - strand, within yjeBat 4404.379 kb on - strand, within yjeBat 4404.490 kb on - strand, within yjeBat 4404.536 kb on + strand, within yjeBat 4404.536 kb on + strand, within yjeBat 4404.536 kb on + strand, within yjeBat 4404.536 kb on + strand, within yjeBat 4404.536 kb on + strand, within yjeBat 4404.544 kb on - strand, within yjeBat 4404.544 kb on - strand, within yjeBat 4404.567 kb on + strand, within yjeBat 4404.583 kb on - strand, within yjeBat 4404.600 kb on - strandat 4404.620 kb on - strandat 4404.695 kb on + strandat 4404.695 kb on + strandat 4404.806 kb on - strandat 4404.819 kb on - strandat 4404.873 kb on - strandat 4404.898 kb on + strandat 4404.898 kb on + strandat 4404.898 kb on + strandat 4404.928 kb on + strand, within vacBat 4404.928 kb on + strand, within vacBat 4404.952 kb on + strand, within vacBat 4404.966 kb on + strand, within vacBat 4404.966 kb on + strand, within vacBat 4405.013 kb on - strand, within vacBat 4405.066 kb on - strand, within vacBat 4405.066 kb on - strand, within vacBat 4405.080 kb on - strand, within vacBat 4405.151 kb on - strand, within vacBat 4405.187 kb on + strand, within vacBat 4405.245 kb on + strand, within vacBat 4405.278 kb on - strand, within vacBat 4405.313 kb on + strand, within vacBat 4405.348 kb on + strand, within vacBat 4405.358 kb on - strand, within vacBat 4405.463 kb on - strand, within vacBat 4405.463 kb on - strand, within vacBat 4405.580 kb on + strand, within vacBat 4405.590 kb on + strand, within vacBat 4405.590 kb on + strand, within vacBat 4405.590 kb on + strand, within vacBat 4405.664 kb on + strand, within vacBat 4405.697 kb on - strand, within vacBat 4405.717 kb on + strand, within vacBat 4405.736 kb on + strand, within vacBat 4405.736 kb on + strand, within vacBat 4405.844 kb on + strand, within vacBat 4405.844 kb on + strand, within vacBat 4405.863 kb on + strand, within vacBat 4405.871 kb on - strand, within vacBat 4405.906 kb on + strand, within vacBat 4405.906 kb on + strand, within vacBat 4405.992 kb on + strand, within vacBat 4406.112 kb on + strand, within vacBat 4406.130 kb on - strand, within vacBat 4406.140 kb on + strand, within vacBat 4406.177 kb on - strand, within vacBat 4406.286 kb on - strand, within vacBat 4406.286 kb on - strand, within vacBat 4406.330 kb on - strand, within vacBat 4406.455 kb on + strand, within vacBat 4406.455 kb on + strand, within vacBat 4406.465 kb on + strand, within vacBat 4406.465 kb on + strand, within vacBat 4406.471 kb on - strand, within vacBat 4406.516 kb on - strand, within vacBat 4406.518 kb on + strand, within vacBat 4406.523 kb on + strand, within vacBat 4406.526 kb on - strand, within vacBat 4406.529 kb on - strand, within vacBat 4406.564 kb on + strand, within vacBat 4406.727 kb on - strand, within vacBat 4406.727 kb on - strand, within vacBat 4406.743 kb on - strand, within vacBat 4406.812 kb on + strand, within vacBat 4406.846 kb on - strand, within vacBat 4406.846 kb on - strand, within vacBat 4407.253 kb on + strandat 4407.286 kb on - strandat 4407.328 kb on + strandat 4407.335 kb on + strandat 4407.335 kb on + strandat 4407.336 kb on - strandat 4407.336 kb on - strandat 4407.336 kb on - strandat 4407.336 kb on - strandat 4407.345 kb on - strandat 4407.345 kb on - strandat 4407.346 kb on - strandat 4407.404 kb on + strand, within rlmBat 4407.404 kb on + strand, within rlmBat 4407.501 kb on + strand, within rlmBat 4407.534 kb on - strand, within rlmBat 4407.534 kb on - strandat 4407.704 kb on - strand, within rlmBat 4407.772 kb on - strand, within rlmBat 4407.772 kb on - strand, within rlmBat 4407.776 kb on + strand, within rlmBat 4407.776 kb on + strand, within rlmBat 4407.833 kb on + strand, within rlmBat 4407.842 kb on - strand, within rlmBat 4407.849 kb on - strand, within rlmBat 4407.874 kb on + strand, within rlmBat 4407.876 kb on + strand, within rlmBat 4407.876 kb on + strand, within rlmBat 4407.882 kb on - strand, within rlmBat 4407.889 kb on + strand, within rlmBat 4407.891 kb on - strand, within rlmBat 4407.898 kb on + strand, within rlmBat 4407.906 kb on + strand, within rlmBat 4407.907 kb on + strand, within rlmBat 4407.924 kb on - strand, within rlmBat 4407.941 kb on + strand, within rlmBat 4407.942 kb on - strand, within rlmBat 4407.942 kb on - strand, within rlmBat 4407.951 kb on + strand, within rlmBat 4407.953 kb on - strand, within rlmBat 4407.967 kb on + strandat 4407.967 kb on + strandat 4407.977 kb on + strandat 4408.012 kb on + strandat 4408.012 kb on + strandat 4408.012 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction methylglyoxal 0.008 vol%
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4,403,855 - purA b4177 0.88 +0.1
4,403,965 + +1.2
4,403,967 - -0.3
4,404,050 + +1.2
4,404,050 + +0.1
4,404,050 + +0.4
4,404,050 + +3.0
4,404,070 - -3.3
4,404,095 - -0.7
4,404,095 - -3.1
4,404,097 + +2.4
4,404,097 + +1.1
4,404,169 - -2.2
4,404,254 + -0.3
4,404,311 + yjeB b4178 0.23 -0.3
4,404,311 + yjeB b4178 0.23 -0.9
4,404,312 + yjeB b4178 0.23 +1.7
4,404,312 + yjeB b4178 0.23 +0.6
4,404,364 - yjeB b4178 0.35 +0.1
4,404,364 - yjeB b4178 0.35 -1.9
4,404,379 - yjeB b4178 0.39 -1.7
4,404,379 - yjeB b4178 0.39 -0.5
4,404,490 - yjeB b4178 0.65 -1.2
4,404,536 + yjeB b4178 0.76 +1.3
4,404,536 + yjeB b4178 0.76 +1.1
4,404,536 + yjeB b4178 0.76 -2.1
4,404,536 + yjeB b4178 0.76 +1.5
4,404,536 + yjeB b4178 0.76 +1.7
4,404,544 - yjeB b4178 0.78 -1.5
4,404,544 - yjeB b4178 0.78 +0.5
4,404,567 + yjeB b4178 0.83 +0.6
4,404,583 - yjeB b4178 0.87 -2.4
4,404,600 - -1.9
4,404,620 - +1.1
4,404,695 + -2.1
4,404,695 + -2.1
4,404,806 - +0.1
4,404,819 - +0.1
4,404,873 - -2.1
4,404,898 + +1.1
4,404,898 + -1.1
4,404,898 + -1.5
4,404,928 + vacB b4179 0.10 +2.1
4,404,928 + vacB b4179 0.10 -0.5
4,404,952 + vacB b4179 0.11 +2.1
4,404,966 + vacB b4179 0.12 -2.1
4,404,966 + vacB b4179 0.12 -1.7
4,405,013 - vacB b4179 0.14 -1.2
4,405,066 - vacB b4179 0.16 +0.1
4,405,066 - vacB b4179 0.16 -1.5
4,405,080 - vacB b4179 0.17 +1.1
4,405,151 - vacB b4179 0.19 -0.9
4,405,187 + vacB b4179 0.21 -0.9
4,405,245 + vacB b4179 0.23 -1.7
4,405,278 - vacB b4179 0.25 -3.5
4,405,313 + vacB b4179 0.26 -0.5
4,405,348 + vacB b4179 0.27 -2.2
4,405,358 - vacB b4179 0.28 -2.0
4,405,463 - vacB b4179 0.32 -2.2
4,405,463 - vacB b4179 0.32 -1.7
4,405,580 + vacB b4179 0.37 -0.9
4,405,590 + vacB b4179 0.37 -2.7
4,405,590 + vacB b4179 0.37 +0.1
4,405,590 + vacB b4179 0.37 +1.1
4,405,664 + vacB b4179 0.40 -0.5
4,405,697 - vacB b4179 0.42 -1.5
4,405,717 + vacB b4179 0.43 -1.9
4,405,736 + vacB b4179 0.43 +1.1
4,405,736 + vacB b4179 0.43 -0.9
4,405,844 + vacB b4179 0.48 -1.7
4,405,844 + vacB b4179 0.48 -1.9
4,405,863 + vacB b4179 0.49 +0.1
4,405,871 - vacB b4179 0.49 -2.4
4,405,906 + vacB b4179 0.50 -2.5
4,405,906 + vacB b4179 0.50 -0.5
4,405,992 + vacB b4179 0.54 -2.6
4,406,112 + vacB b4179 0.59 -1.7
4,406,130 - vacB b4179 0.60 -0.5
4,406,140 + vacB b4179 0.60 -1.7
4,406,177 - vacB b4179 0.61 -0.9
4,406,286 - vacB b4179 0.66 -1.2
4,406,286 - vacB b4179 0.66 -0.9
4,406,330 - vacB b4179 0.68 +0.1
4,406,455 + vacB b4179 0.73 -0.9
4,406,455 + vacB b4179 0.73 -1.5
4,406,465 + vacB b4179 0.73 -0.5
4,406,465 + vacB b4179 0.73 -1.9
4,406,471 - vacB b4179 0.73 +1.1
4,406,516 - vacB b4179 0.75 -0.5
4,406,518 + vacB b4179 0.75 -1.9
4,406,523 + vacB b4179 0.76 -0.7
4,406,526 - vacB b4179 0.76 -0.5
4,406,529 - vacB b4179 0.76 -0.5
4,406,564 + vacB b4179 0.77 -0.7
4,406,727 - vacB b4179 0.84 -2.1
4,406,727 - vacB b4179 0.84 -2.1
4,406,743 - vacB b4179 0.85 -1.2
4,406,812 + vacB b4179 0.87 -1.2
4,406,846 - vacB b4179 0.89 -2.2
4,406,846 - vacB b4179 0.89 -3.3
4,407,253 + -0.0
4,407,286 - -0.5
4,407,328 + +0.0
4,407,335 + -0.6
4,407,335 + +0.1
4,407,336 - +0.7
4,407,336 - -1.2
4,407,336 - -0.6
4,407,336 - +0.4
4,407,345 - -1.4
4,407,345 - -1.3
4,407,346 - +0.7
4,407,404 + rlmB b4180 0.14 +0.1
4,407,404 + rlmB b4180 0.14 +1.1
4,407,501 + rlmB b4180 0.28 -0.9
4,407,534 - rlmB b4180 0.32 -0.5
4,407,534 - -1.2
4,407,704 - rlmB b4180 0.55 +1.5
4,407,772 - rlmB b4180 0.65 +0.5
4,407,772 - rlmB b4180 0.65 +0.5
4,407,776 + rlmB b4180 0.65 +0.4
4,407,776 + rlmB b4180 0.65 -0.2
4,407,833 + rlmB b4180 0.73 -2.2
4,407,842 - rlmB b4180 0.74 -2.5
4,407,849 - rlmB b4180 0.75 -1.5
4,407,874 + rlmB b4180 0.79 +2.1
4,407,876 + rlmB b4180 0.79 -0.8
4,407,876 + rlmB b4180 0.79 +0.5
4,407,882 - rlmB b4180 0.80 -0.9
4,407,889 + rlmB b4180 0.81 -0.6
4,407,891 - rlmB b4180 0.81 -0.0
4,407,898 + rlmB b4180 0.82 -2.1
4,407,906 + rlmB b4180 0.83 -0.5
4,407,907 + rlmB b4180 0.83 +0.1
4,407,924 - rlmB b4180 0.86 -0.5
4,407,941 + rlmB b4180 0.88 +0.1
4,407,942 - rlmB b4180 0.88 +2.4
4,407,942 - rlmB b4180 0.88 -0.2
4,407,951 + rlmB b4180 0.89 -1.5
4,407,953 - rlmB b4180 0.89 +0.7
4,407,967 + -1.2
4,407,967 + -0.8
4,407,977 + -1.1
4,408,012 + -0.5
4,408,012 + +0.6
4,408,012 - +0.3

Or see this region's nucleotide sequence