Experiment: methylglyoxal 0.008 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt truB and rbfA overlap by 1 nucleotides rbfA and infB are separated by 163 nucleotides infB and nusA are separated by 24 nucleotides
b3166: truB - tRNA pseudouridine synthase B (NCBI), at 3,309,855 to 3,310,799
truB
b3167: rbfA - ribosome-binding factor A (NCBI), at 3,310,799 to 3,311,200
rbfA
b3168: infB - translation initiation factor IF-2 (NCBI), at 3,311,364 to 3,314,036
infB
b3169: nusA - transcription elongation factor NusA (NCBI), at 3,314,061 to 3,315,548
nusA
Position (kb)
3311
3312
3313
3314
3315 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3310.371 kb on - strand, within truB at 3310.487 kb on + strand, within truB at 3310.523 kb on - strand, within truB at 3310.539 kb on + strand, within truB at 3310.545 kb on - strand, within truB at 3310.562 kb on + strand, within truB at 3310.562 kb on + strand, within truB at 3310.587 kb on + strand, within truB at 3310.655 kb on + strand, within truB at 3310.655 kb on + strand, within truB at 3310.682 kb on + strand, within truB at 3310.695 kb on - strand, within truB at 3310.700 kb on - strand, within truB at 3310.700 kb on - strand, within truB at 3310.723 kb on + strand at 3310.731 kb on - strand at 3310.736 kb on - strand at 3310.800 kb on - strand at 3310.800 kb on - strand at 3310.805 kb on - strand at 3310.845 kb on + strand, within rbfA at 3310.909 kb on - strand, within rbfA at 3311.290 kb on - strand at 3311.290 kb on - strand at 3311.382 kb on - strand at 3311.409 kb on - strand at 3311.409 kb on - strand at 3313.766 kb on - strand, within infB at 3313.842 kb on - strand at 3313.932 kb on - strand at 3313.976 kb on - strand at 3314.038 kb on - strand at 3314.051 kb on - strand at 3314.051 kb on - strand at 3314.233 kb on - strand, within nusA at 3314.249 kb on - strand, within nusA at 3314.252 kb on - strand, within nusA at 3314.302 kb on - strand, within nusA at 3314.302 kb on - strand, within nusA at 3314.302 kb on - strand, within nusA
Per-strain Table
Position Strand Gene LocusTag Fraction methylglyoxal 0.008 vol% remove 3,310,371 - truB b3166 0.55 -0.3 3,310,487 + truB b3166 0.67 +0.3 3,310,523 - truB b3166 0.71 -1.9 3,310,539 + truB b3166 0.72 -1.4 3,310,545 - truB b3166 0.73 -0.9 3,310,562 + truB b3166 0.75 -1.6 3,310,562 + truB b3166 0.75 +0.0 3,310,587 + truB b3166 0.77 -1.0 3,310,655 + truB b3166 0.85 -0.6 3,310,655 + truB b3166 0.85 +0.6 3,310,682 + truB b3166 0.88 -2.0 3,310,695 - truB b3166 0.89 +1.0 3,310,700 - truB b3166 0.89 -0.2 3,310,700 - truB b3166 0.89 -1.3 3,310,723 + -0.2 3,310,731 - -1.8 3,310,736 - -0.7 3,310,800 - -0.8 3,310,800 - -0.2 3,310,805 - +0.0 3,310,845 + rbfA b3167 0.11 -2.2 3,310,909 - rbfA b3167 0.27 -1.6 3,311,290 - -1.3 3,311,290 - +0.4 3,311,382 - +0.4 3,311,409 - +1.2 3,311,409 - +1.0 3,313,766 - infB b3168 0.90 +0.4 3,313,842 - -0.2 3,313,932 - +1.0 3,313,976 - -1.8 3,314,038 - +0.2 3,314,051 - -0.2 3,314,051 - +0.7 3,314,233 - nusA b3169 0.12 +2.0 3,314,249 - nusA b3169 0.13 +3.4 3,314,252 - nusA b3169 0.13 +2.6 3,314,302 - nusA b3169 0.16 +1.2 3,314,302 - nusA b3169 0.16 +1.7 3,314,302 - nusA b3169 0.16 +1.4
Or see this region's nucleotide sequence