Experiment: methylglyoxal 0.008 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yojI and alkB are separated by 75 nucleotides alkB and ada overlap by 1 nucleotides
b2211: yojI - fused predicted multidrug transport subunits of ABC superfamily: membrane component/ATP-binding component (NCBI), at 2,304,994 to 2,306,637
yojI
b2212: alkB - oxidative demethylase of N1-methyladenine or N3-methylcytosine DNA lesions (NCBI), at 2,306,713 to 2,307,363
alkB
b2213: ada - fused DNA-binding transcriptional dual regulator/O6-methylguanine-DNA methyltransferase (NCBI), at 2,307,363 to 2,308,427
ada
Position (kb)
2306
2307
2308 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2305.720 kb on - strand, within yojI at 2305.720 kb on - strand, within yojI at 2305.747 kb on + strand, within yojI at 2305.747 kb on + strand, within yojI at 2305.760 kb on + strand, within yojI at 2305.763 kb on + strand, within yojI at 2305.799 kb on - strand, within yojI at 2305.829 kb on + strand, within yojI at 2305.848 kb on + strand, within yojI at 2305.890 kb on - strand, within yojI at 2305.935 kb on - strand, within yojI at 2306.040 kb on - strand, within yojI at 2306.078 kb on + strand, within yojI at 2306.139 kb on + strand, within yojI at 2306.139 kb on + strand, within yojI at 2306.337 kb on + strand, within yojI at 2306.337 kb on + strand, within yojI at 2306.433 kb on - strand, within yojI at 2306.444 kb on - strand, within yojI at 2306.444 kb on - strand, within yojI at 2306.444 kb on - strand, within yojI at 2306.500 kb on + strand at 2306.572 kb on + strand at 2306.572 kb on + strand at 2306.783 kb on + strand, within alkB at 2306.801 kb on - strand, within alkB at 2306.942 kb on - strand, within alkB at 2306.942 kb on - strand, within alkB at 2307.059 kb on + strand, within alkB at 2307.168 kb on - strand, within alkB at 2307.181 kb on - strand, within alkB at 2307.336 kb on + strand at 2307.336 kb on + strand at 2307.336 kb on + strand at 2307.368 kb on - strand at 2307.393 kb on + strand at 2307.549 kb on - strand, within ada at 2307.562 kb on - strand, within ada at 2307.583 kb on + strand, within ada at 2307.593 kb on + strand, within ada at 2307.849 kb on - strand, within ada at 2308.153 kb on + strand, within ada at 2308.153 kb on + strand, within ada at 2308.162 kb on + strand, within ada at 2308.327 kb on + strand at 2308.337 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction methylglyoxal 0.008 vol% remove 2,305,720 - yojI b2211 0.44 +0.0 2,305,720 - yojI b2211 0.44 +0.3 2,305,747 + yojI b2211 0.46 +1.3 2,305,747 + yojI b2211 0.46 +1.5 2,305,760 + yojI b2211 0.47 +0.1 2,305,763 + yojI b2211 0.47 -0.9 2,305,799 - yojI b2211 0.49 +0.3 2,305,829 + yojI b2211 0.51 +0.2 2,305,848 + yojI b2211 0.52 +0.2 2,305,890 - yojI b2211 0.55 +0.5 2,305,935 - yojI b2211 0.57 +0.7 2,306,040 - yojI b2211 0.64 -0.5 2,306,078 + yojI b2211 0.66 +0.6 2,306,139 + yojI b2211 0.70 +0.4 2,306,139 + yojI b2211 0.70 +1.1 2,306,337 + yojI b2211 0.82 +1.5 2,306,337 + yojI b2211 0.82 +0.1 2,306,433 - yojI b2211 0.88 -0.4 2,306,444 - yojI b2211 0.88 -0.3 2,306,444 - yojI b2211 0.88 +0.6 2,306,444 - yojI b2211 0.88 +0.2 2,306,500 + -0.7 2,306,572 + -0.1 2,306,572 + -2.3 2,306,783 + alkB b2212 0.11 -1.5 2,306,801 - alkB b2212 0.14 -1.5 2,306,942 - alkB b2212 0.35 -1.5 2,306,942 - alkB b2212 0.35 -0.4 2,307,059 + alkB b2212 0.53 -1.1 2,307,168 - alkB b2212 0.70 -0.2 2,307,181 - alkB b2212 0.72 -0.5 2,307,336 + -0.1 2,307,336 + +0.3 2,307,336 + +0.3 2,307,368 - +1.9 2,307,393 + -1.8 2,307,549 - ada b2213 0.17 +0.2 2,307,562 - ada b2213 0.19 -0.1 2,307,583 + ada b2213 0.21 +0.1 2,307,593 + ada b2213 0.22 -0.4 2,307,849 - ada b2213 0.46 -0.1 2,308,153 + ada b2213 0.74 -1.8 2,308,153 + ada b2213 0.74 -1.1 2,308,162 + ada b2213 0.75 +1.9 2,308,327 + +1.6 2,308,337 - -1.7
Or see this region's nucleotide sequence