Experiment: methylglyoxal 0.008 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt citG and citX overlap by 26 nucleotides citX and citF are separated by 3 nucleotides citF and citE are separated by 10 nucleotides citE and citD overlap by 4 nucleotides
b0613: citG - triphosphoribosyl-dephospho-CoA transferase (NCBI), at 645,854 to 646,732
citG
b0614: citX - 2'-(5'-triphosphoribosyl)-3'-dephospho-CoA:apo- citrate lyase (NCBI), at 646,707 to 647,258
citX
b0615: citF - citrate lyase, citrate-ACP transferase (alpha) subunit (NCBI), at 647,262 to 648,794
citF
b0616: citE - citrate lyase beta chain (acyl lyase subunit) (VIMSS), at 648,805 to 649,713
citE
b0617: citD - citrate lyase, acyl carrier (gamma) subunit (NCBI), at 649,710 to 650,006
citD
Position (kb) 647
648
649 Strain fitness (log2 ratio) -1
0
1
2 at 646.308 kb on - strand, within citG at 646.308 kb on - strand, within citG at 646.530 kb on - strand, within citG at 646.595 kb on + strand, within citG at 646.595 kb on + strand, within citG at 646.656 kb on + strand at 646.666 kb on + strand at 646.666 kb on + strand at 646.675 kb on - strand at 646.675 kb on - strand at 647.268 kb on - strand at 647.354 kb on + strand at 647.383 kb on - strand at 647.428 kb on + strand, within citF at 647.428 kb on + strand, within citF at 647.522 kb on + strand, within citF at 647.601 kb on + strand, within citF at 648.197 kb on + strand, within citF at 648.197 kb on + strand, within citF at 648.551 kb on - strand, within citF at 648.551 kb on - strand, within citF at 648.610 kb on - strand, within citF at 648.645 kb on - strand at 648.676 kb on + strand at 648.676 kb on + strand at 648.810 kb on - strand at 649.122 kb on - strand, within citE at 649.238 kb on - strand, within citE at 649.238 kb on - strand, within citE at 649.327 kb on - strand, within citE at 649.479 kb on + strand, within citE at 649.540 kb on - strand, within citE at 649.569 kb on + strand, within citE at 649.595 kb on - strand, within citE at 649.595 kb on - strand, within citE at 649.607 kb on + strand, within citE at 649.642 kb on + strand at 649.665 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction methylglyoxal 0.008 vol% remove 646,308 - citG b0613 0.52 +1.4 646,308 - citG b0613 0.52 +0.7 646,530 - citG b0613 0.77 -1.4 646,595 + citG b0613 0.84 -0.7 646,595 + citG b0613 0.84 -0.3 646,656 + +1.2 646,666 + +2.2 646,666 + +0.3 646,675 - +0.1 646,675 - -0.7 647,268 - +0.4 647,354 + +0.6 647,383 - +0.9 647,428 + citF b0615 0.11 -0.3 647,428 + citF b0615 0.11 +0.2 647,522 + citF b0615 0.17 +0.6 647,601 + citF b0615 0.22 +1.9 648,197 + citF b0615 0.61 +1.8 648,197 + citF b0615 0.61 -0.7 648,551 - citF b0615 0.84 +0.9 648,551 - citF b0615 0.84 +2.5 648,610 - citF b0615 0.88 +0.6 648,645 - +0.3 648,676 + +0.9 648,676 + -1.1 648,810 - +0.5 649,122 - citE b0616 0.35 -0.2 649,238 - citE b0616 0.48 -0.1 649,238 - citE b0616 0.48 +0.9 649,327 - citE b0616 0.57 +1.2 649,479 + citE b0616 0.74 -0.4 649,540 - citE b0616 0.81 +0.2 649,569 + citE b0616 0.84 +0.6 649,595 - citE b0616 0.87 -0.4 649,595 - citE b0616 0.87 -0.1 649,607 + citE b0616 0.88 -0.1 649,642 + +0.0 649,665 - -0.6
Or see this region's nucleotide sequence