Strain Fitness in Escherichia coli BW25113 around b0475

Experiment: methylglyoxal 0.008 vol%

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntadk and hemH are separated by 235 nucleotideshemH and aes overlap by 4 nucleotides b0474: adk - adenylate kinase (NCBI), at 496,399 to 497,043 adk b0475: hemH - ferrochelatase (NCBI), at 497,279 to 498,241 hemH b0476: aes - acetyl esterase (NCBI), at 498,238 to 499,197 aes Position (kb) 497 498 499Strain fitness (log2 ratio) -1 0 1at 498.393 kb on + strand, within aesat 498.393 kb on + strand, within aesat 498.461 kb on + strand, within aesat 498.464 kb on + strand, within aesat 498.476 kb on + strand, within aesat 498.490 kb on + strand, within aesat 498.531 kb on - strand, within aesat 498.531 kb on - strand, within aesat 498.546 kb on + strand, within aesat 498.546 kb on + strand, within aesat 498.564 kb on - strand, within aesat 498.564 kb on - strand, within aesat 498.624 kb on - strand, within aesat 498.675 kb on - strand, within aesat 498.730 kb on - strand, within aesat 498.740 kb on + strand, within aesat 498.839 kb on - strand, within aesat 498.894 kb on - strand, within aesat 498.942 kb on + strand, within aesat 498.960 kb on - strand, within aesat 499.132 kb on + strandat 499.138 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction methylglyoxal 0.008 vol%
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498,393 + aes b0476 0.16 +0.9
498,393 + aes b0476 0.16 +1.5
498,461 + aes b0476 0.23 -0.8
498,464 + aes b0476 0.24 -0.1
498,476 + aes b0476 0.25 +0.0
498,490 + aes b0476 0.26 +0.2
498,531 - aes b0476 0.31 -0.1
498,531 - aes b0476 0.31 +0.6
498,546 + aes b0476 0.32 -0.2
498,546 + aes b0476 0.32 +0.7
498,564 - aes b0476 0.34 +0.3
498,564 - aes b0476 0.34 +0.9
498,624 - aes b0476 0.40 +0.1
498,675 - aes b0476 0.46 -1.0
498,730 - aes b0476 0.51 +0.1
498,740 + aes b0476 0.52 +0.7
498,839 - aes b0476 0.63 +0.6
498,894 - aes b0476 0.68 -1.4
498,942 + aes b0476 0.73 -0.7
498,960 - aes b0476 0.75 +0.5
499,132 + +0.6
499,138 - +0.8

Or see this region's nucleotide sequence