Experiment: methylglyoxal 0.008 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fixC and fixX overlap by 4 nucleotides fixX and yaaU are separated by 56 nucleotides yaaU and kefF are separated by 107 nucleotides kefF and kefC overlap by 8 nucleotides
b0043: fixC - predicted oxidoreductase with FAD/NAD(P)-binding domain (NCBI), at 44,180 to 45,466
fixC
b0044: fixX - predicted 4Fe-4S ferredoxin-type protein (NCBI), at 45,463 to 45,750
fixX
b0045: yaaU - predicted transporter (NCBI), at 45,807 to 47,138
yaaU
b0046: kefF - glutathione-regulated potassium-efflux system ancillary protein (NCBI), at 47,246 to 47,776
kefF
b0047: kefC - glutathione-regulated potassium-efflux system protein (NCBI), at 47,769 to 49,631
kefC
Position (kb)
45
46
47
48 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 44.857 kb on - strand, within fixC at 44.862 kb on - strand, within fixC at 44.906 kb on - strand, within fixC at 45.034 kb on + strand, within fixC at 45.034 kb on + strand, within fixC at 45.044 kb on + strand, within fixC at 45.044 kb on + strand, within fixC at 45.232 kb on + strand, within fixC at 45.241 kb on - strand, within fixC at 45.323 kb on - strand, within fixC at 45.339 kb on + strand at 45.339 kb on + strand at 45.340 kb on - strand at 45.426 kb on + strand at 45.546 kb on - strand, within fixX at 45.610 kb on + strand, within fixX at 45.700 kb on - strand, within fixX at 45.704 kb on + strand, within fixX at 45.704 kb on + strand, within fixX at 45.844 kb on + strand at 45.880 kb on - strand at 45.924 kb on + strand at 46.113 kb on + strand, within yaaU at 46.113 kb on + strand, within yaaU at 46.142 kb on - strand, within yaaU at 46.144 kb on - strand, within yaaU at 46.176 kb on - strand, within yaaU at 46.176 kb on - strand, within yaaU at 46.240 kb on + strand, within yaaU at 46.284 kb on - strand, within yaaU at 46.329 kb on + strand, within yaaU at 46.329 kb on + strand, within yaaU at 46.463 kb on + strand, within yaaU at 46.463 kb on + strand, within yaaU at 46.564 kb on + strand, within yaaU at 46.564 kb on + strand, within yaaU at 46.575 kb on + strand, within yaaU at 46.707 kb on - strand, within yaaU at 46.707 kb on - strand, within yaaU at 46.800 kb on + strand, within yaaU at 46.920 kb on - strand, within yaaU at 47.003 kb on + strand, within yaaU at 47.067 kb on + strand at 47.097 kb on - strand at 47.097 kb on - strand at 47.113 kb on - strand at 47.113 kb on - strand at 47.202 kb on - strand at 47.225 kb on + strand at 47.225 kb on + strand at 47.247 kb on - strand at 47.269 kb on + strand at 47.359 kb on - strand, within kefF at 47.364 kb on - strand, within kefF at 47.364 kb on - strand, within kefF at 47.364 kb on - strand, within kefF at 47.364 kb on - strand, within kefF at 47.402 kb on - strand, within kefF at 47.412 kb on - strand, within kefF at 47.446 kb on + strand, within kefF at 47.454 kb on + strand, within kefF at 47.503 kb on + strand, within kefF at 47.504 kb on + strand, within kefF at 47.504 kb on + strand, within kefF at 47.639 kb on + strand, within kefF at 47.651 kb on + strand, within kefF at 47.675 kb on + strand, within kefF at 47.681 kb on + strand, within kefF at 47.681 kb on + strand, within kefF at 47.730 kb on + strand at 47.762 kb on + strand at 47.774 kb on - strand at 47.784 kb on - strand at 47.823 kb on + strand at 47.823 kb on + strand at 47.832 kb on + strand at 47.832 kb on + strand at 47.836 kb on + strand at 47.848 kb on + strand at 47.859 kb on + strand at 47.921 kb on + strand at 47.948 kb on + strand at 47.948 kb on + strand at 47.958 kb on + strand, within kefC at 47.958 kb on + strand, within kefC at 47.977 kb on - strand, within kefC at 48.009 kb on - strand, within kefC at 48.009 kb on - strand, within kefC at 48.009 kb on - strand, within kefC at 48.031 kb on + strand, within kefC
Per-strain Table
Position Strand Gene LocusTag Fraction methylglyoxal 0.008 vol% remove 44,857 - fixC b0043 0.53 -0.3 44,862 - fixC b0043 0.53 +0.1 44,906 - fixC b0043 0.56 -1.5 45,034 + fixC b0043 0.66 -1.3 45,034 + fixC b0043 0.66 -1.8 45,044 + fixC b0043 0.67 -1.2 45,044 + fixC b0043 0.67 -0.2 45,232 + fixC b0043 0.82 +0.2 45,241 - fixC b0043 0.82 -0.3 45,323 - fixC b0043 0.89 -0.8 45,339 + +1.3 45,339 + -0.0 45,340 - -1.3 45,426 + +1.7 45,546 - fixX b0044 0.29 +0.3 45,610 + fixX b0044 0.51 +0.4 45,700 - fixX b0044 0.82 +0.6 45,704 + fixX b0044 0.84 +0.1 45,704 + fixX b0044 0.84 +1.3 45,844 + +0.2 45,880 - -1.6 45,924 + +0.4 46,113 + yaaU b0045 0.23 +0.3 46,113 + yaaU b0045 0.23 -0.0 46,142 - yaaU b0045 0.25 -0.5 46,144 - yaaU b0045 0.25 +0.4 46,176 - yaaU b0045 0.28 +2.0 46,176 - yaaU b0045 0.28 -0.3 46,240 + yaaU b0045 0.33 -0.5 46,284 - yaaU b0045 0.36 +0.6 46,329 + yaaU b0045 0.39 -0.5 46,329 + yaaU b0045 0.39 -0.3 46,463 + yaaU b0045 0.49 -0.6 46,463 + yaaU b0045 0.49 +1.0 46,564 + yaaU b0045 0.57 -0.5 46,564 + yaaU b0045 0.57 -0.8 46,575 + yaaU b0045 0.58 -0.3 46,707 - yaaU b0045 0.68 -0.7 46,707 - yaaU b0045 0.68 -1.3 46,800 + yaaU b0045 0.75 +1.0 46,920 - yaaU b0045 0.84 -1.0 47,003 + yaaU b0045 0.90 +1.0 47,067 + -0.7 47,097 - -0.8 47,097 - +0.2 47,113 - +0.5 47,113 - +1.6 47,202 - +2.0 47,225 + +0.2 47,225 + -0.3 47,247 - +0.1 47,269 + -0.2 47,359 - kefF b0046 0.21 +0.5 47,364 - kefF b0046 0.22 +0.6 47,364 - kefF b0046 0.22 -0.3 47,364 - kefF b0046 0.22 +0.3 47,364 - kefF b0046 0.22 -0.5 47,402 - kefF b0046 0.29 +0.8 47,412 - kefF b0046 0.31 -0.9 47,446 + kefF b0046 0.38 +0.6 47,454 + kefF b0046 0.39 +0.6 47,503 + kefF b0046 0.48 +3.0 47,504 + kefF b0046 0.49 -1.2 47,504 + kefF b0046 0.49 +0.6 47,639 + kefF b0046 0.74 +0.1 47,651 + kefF b0046 0.76 +0.1 47,675 + kefF b0046 0.81 +0.7 47,681 + kefF b0046 0.82 +0.4 47,681 + kefF b0046 0.82 -0.8 47,730 + +0.0 47,762 + -0.8 47,774 - -2.7 47,784 - -1.0 47,823 + -0.0 47,823 + -2.3 47,832 + +1.3 47,832 + +1.0 47,836 + +1.0 47,848 + -0.5 47,859 + +0.3 47,921 + -0.6 47,948 + +0.7 47,948 + +0.6 47,958 + kefC b0047 0.10 +0.6 47,958 + kefC b0047 0.10 +1.4 47,977 - kefC b0047 0.11 +0.4 48,009 - kefC b0047 0.13 -1.0 48,009 - kefC b0047 0.13 -0.2 48,009 - kefC b0047 0.13 +2.3 48,031 + kefC b0047 0.14 -1.1
Or see this region's nucleotide sequence