Experiment: L-Alanine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PfGW456L13_219 and PfGW456L13_220 are separated by 243 nucleotides PfGW456L13_220 and PfGW456L13_221 are separated by 427 nucleotides PfGW456L13_221 and PfGW456L13_222 are separated by 332 nucleotides PfGW456L13_222 and PfGW456L13_223 are separated by 12 nucleotides
PfGW456L13_219 - Protein involved in catabolism of external DNA, at 256,789 to 257,628
_219
PfGW456L13_220 - hypothetical protein, at 257,872 to 258,108
_220
PfGW456L13_221 - Proline/sodium symporter PutP (TC 2.A.21.2.1) @ Propionate/sodium symporter, at 258,536 to 260,020
_221
PfGW456L13_222 - hypothetical protein, at 260,353 to 260,469
_222
PfGW456L13_223 - Transcriptional repressor of PutA and PutP / Proline dehydrogenase (EC 1.5.99.8) (Proline oxidase) / Delta-1-pyrroline-5-carboxylate dehydrogenase (EC 1.5.1.12), at 260,482 to 264,432
_223
Position (kb) 258
259
260
261 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 257.621 kb on + strand at 257.894 kb on - strand at 257.894 kb on - strand at 257.940 kb on + strand, within PfGW456L13_220 at 257.940 kb on + strand, within PfGW456L13_220 at 257.940 kb on + strand, within PfGW456L13_220 at 257.940 kb on + strand, within PfGW456L13_220 at 257.941 kb on - strand, within PfGW456L13_220 at 257.941 kb on - strand, within PfGW456L13_220 at 257.941 kb on - strand, within PfGW456L13_220 at 257.941 kb on - strand, within PfGW456L13_220 at 257.941 kb on - strand, within PfGW456L13_220 at 258.019 kb on - strand, within PfGW456L13_220 at 258.019 kb on - strand, within PfGW456L13_220 at 258.113 kb on + strand at 258.113 kb on + strand at 258.123 kb on + strand at 258.123 kb on + strand at 258.151 kb on - strand at 258.151 kb on - strand at 258.187 kb on - strand at 258.317 kb on + strand at 258.317 kb on + strand at 258.318 kb on - strand at 258.318 kb on - strand at 258.318 kb on - strand at 258.481 kb on + strand at 258.482 kb on - strand at 258.552 kb on + strand at 258.621 kb on + strand at 258.622 kb on - strand at 258.622 kb on - strand at 258.932 kb on - strand, within PfGW456L13_221 at 258.948 kb on + strand, within PfGW456L13_221 at 258.948 kb on + strand, within PfGW456L13_221 at 258.948 kb on + strand, within PfGW456L13_221 at 258.948 kb on + strand, within PfGW456L13_221 at 258.949 kb on - strand, within PfGW456L13_221 at 258.949 kb on - strand, within PfGW456L13_221 at 258.949 kb on - strand, within PfGW456L13_221 at 259.137 kb on + strand, within PfGW456L13_221 at 259.138 kb on - strand, within PfGW456L13_221 at 259.138 kb on - strand, within PfGW456L13_221 at 259.228 kb on + strand, within PfGW456L13_221 at 259.278 kb on + strand, within PfGW456L13_221 at 259.278 kb on + strand, within PfGW456L13_221 at 259.279 kb on - strand, within PfGW456L13_221 at 259.279 kb on - strand, within PfGW456L13_221 at 259.454 kb on + strand, within PfGW456L13_221 at 259.454 kb on + strand, within PfGW456L13_221 at 259.454 kb on + strand, within PfGW456L13_221 at 259.454 kb on + strand, within PfGW456L13_221 at 259.454 kb on + strand, within PfGW456L13_221 at 259.455 kb on - strand, within PfGW456L13_221 at 259.500 kb on + strand, within PfGW456L13_221 at 259.500 kb on + strand, within PfGW456L13_221 at 259.500 kb on + strand, within PfGW456L13_221 at 259.500 kb on + strand, within PfGW456L13_221 at 259.500 kb on + strand, within PfGW456L13_221 at 259.501 kb on - strand, within PfGW456L13_221 at 259.501 kb on - strand, within PfGW456L13_221 at 259.602 kb on + strand, within PfGW456L13_221 at 259.602 kb on + strand, within PfGW456L13_221 at 259.602 kb on + strand, within PfGW456L13_221 at 259.602 kb on + strand, within PfGW456L13_221 at 259.602 kb on + strand, within PfGW456L13_221 at 259.602 kb on + strand, within PfGW456L13_221 at 259.602 kb on + strand, within PfGW456L13_221 at 259.602 kb on + strand, within PfGW456L13_221 at 259.602 kb on + strand, within PfGW456L13_221 at 259.602 kb on + strand, within PfGW456L13_221 at 259.602 kb on + strand, within PfGW456L13_221 at 259.602 kb on + strand, within PfGW456L13_221 at 259.602 kb on + strand, within PfGW456L13_221 at 259.602 kb on + strand, within PfGW456L13_221 at 259.602 kb on + strand, within PfGW456L13_221 at 259.602 kb on + strand, within PfGW456L13_221 at 259.602 kb on + strand, within PfGW456L13_221 at 259.602 kb on + strand, within PfGW456L13_221 at 259.602 kb on + strand, within PfGW456L13_221 at 259.602 kb on + strand, within PfGW456L13_221 at 259.603 kb on - strand, within PfGW456L13_221 at 259.603 kb on - strand, within PfGW456L13_221 at 259.603 kb on - strand, within PfGW456L13_221 at 259.603 kb on - strand, within PfGW456L13_221 at 259.603 kb on - strand, within PfGW456L13_221 at 259.603 kb on - strand, within PfGW456L13_221 at 259.603 kb on - strand, within PfGW456L13_221 at 259.603 kb on - strand, within PfGW456L13_221 at 259.603 kb on - strand, within PfGW456L13_221 at 259.603 kb on - strand, within PfGW456L13_221 at 259.603 kb on - strand, within PfGW456L13_221 at 259.603 kb on - strand, within PfGW456L13_221 at 259.603 kb on - strand, within PfGW456L13_221 at 259.603 kb on - strand, within PfGW456L13_221 at 259.603 kb on - strand, within PfGW456L13_221 at 259.603 kb on - strand, within PfGW456L13_221 at 259.684 kb on - strand, within PfGW456L13_221 at 259.762 kb on - strand, within PfGW456L13_221 at 259.815 kb on + strand, within PfGW456L13_221 at 259.815 kb on + strand, within PfGW456L13_221 at 259.815 kb on + strand, within PfGW456L13_221 at 259.815 kb on + strand, within PfGW456L13_221 at 259.815 kb on + strand, within PfGW456L13_221 at 259.815 kb on + strand, within PfGW456L13_221 at 259.815 kb on + strand, within PfGW456L13_221 at 259.815 kb on + strand, within PfGW456L13_221 at 259.815 kb on + strand, within PfGW456L13_221 at 259.815 kb on + strand, within PfGW456L13_221 at 259.815 kb on + strand, within PfGW456L13_221 at 259.816 kb on - strand, within PfGW456L13_221 at 259.816 kb on - strand, within PfGW456L13_221 at 259.816 kb on - strand, within PfGW456L13_221 at 259.816 kb on - strand, within PfGW456L13_221 at 259.816 kb on - strand, within PfGW456L13_221 at 259.816 kb on - strand, within PfGW456L13_221 at 259.816 kb on - strand, within PfGW456L13_221 at 259.816 kb on - strand, within PfGW456L13_221 at 259.816 kb on - strand, within PfGW456L13_221 at 259.917 kb on + strand at 259.980 kb on + strand at 259.980 kb on + strand at 259.980 kb on + strand at 259.981 kb on - strand at 259.981 kb on - strand at 259.981 kb on - strand at 260.012 kb on + strand at 260.013 kb on - strand at 260.013 kb on - strand at 260.021 kb on + strand at 260.021 kb on + strand at 260.021 kb on + strand at 260.022 kb on - strand at 260.022 kb on - strand at 260.022 kb on - strand at 260.026 kb on + strand at 260.026 kb on + strand at 260.045 kb on + strand at 260.045 kb on + strand at 260.045 kb on + strand at 260.045 kb on + strand at 260.045 kb on + strand at 260.046 kb on - strand at 260.046 kb on - strand at 260.046 kb on - strand at 260.046 kb on - strand at 260.049 kb on + strand at 260.049 kb on + strand at 260.050 kb on - strand at 260.050 kb on - strand at 260.081 kb on + strand at 260.081 kb on + strand at 260.082 kb on - strand at 260.082 kb on - strand at 260.082 kb on - strand at 260.116 kb on - strand at 260.116 kb on - strand at 260.116 kb on - strand at 260.116 kb on - strand at 260.116 kb on - strand at 260.194 kb on - strand at 260.194 kb on - strand at 260.194 kb on - strand at 260.209 kb on + strand at 260.210 kb on - strand at 260.234 kb on + strand at 260.313 kb on + strand at 260.354 kb on + strand at 260.355 kb on - strand at 260.376 kb on + strand, within PfGW456L13_222 at 260.599 kb on + strand at 260.599 kb on + strand at 260.600 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Alanine (C) remove 257,621 + -0.7 257,894 - +1.7 257,894 - -0.6 257,940 + PfGW456L13_220 0.29 +1.1 257,940 + PfGW456L13_220 0.29 +1.0 257,940 + PfGW456L13_220 0.29 +0.1 257,940 + PfGW456L13_220 0.29 +0.1 257,941 - PfGW456L13_220 0.29 -0.5 257,941 - PfGW456L13_220 0.29 +1.3 257,941 - PfGW456L13_220 0.29 -0.6 257,941 - PfGW456L13_220 0.29 -0.1 257,941 - PfGW456L13_220 0.29 +0.6 258,019 - PfGW456L13_220 0.62 -0.8 258,019 - PfGW456L13_220 0.62 +0.8 258,113 + -0.7 258,113 + -3.6 258,123 + +0.7 258,123 + -0.4 258,151 - -0.5 258,151 - +0.8 258,187 - -0.3 258,317 + -1.4 258,317 + -1.2 258,318 - +0.7 258,318 - -1.5 258,318 - +1.4 258,481 + -1.5 258,482 - +0.8 258,552 + +0.6 258,621 + +0.5 258,622 - -0.2 258,622 - -0.5 258,932 - PfGW456L13_221 0.27 +0.9 258,948 + PfGW456L13_221 0.28 -0.6 258,948 + PfGW456L13_221 0.28 -2.9 258,948 + PfGW456L13_221 0.28 -1.7 258,948 + PfGW456L13_221 0.28 -0.3 258,949 - PfGW456L13_221 0.28 -0.5 258,949 - PfGW456L13_221 0.28 -1.1 258,949 - PfGW456L13_221 0.28 -1.7 259,137 + PfGW456L13_221 0.40 +0.0 259,138 - PfGW456L13_221 0.41 +0.8 259,138 - PfGW456L13_221 0.41 -0.7 259,228 + PfGW456L13_221 0.47 -0.5 259,278 + PfGW456L13_221 0.50 -1.8 259,278 + PfGW456L13_221 0.50 +0.7 259,279 - PfGW456L13_221 0.50 -0.3 259,279 - PfGW456L13_221 0.50 -0.4 259,454 + PfGW456L13_221 0.62 +0.5 259,454 + PfGW456L13_221 0.62 +0.3 259,454 + PfGW456L13_221 0.62 -0.4 259,454 + PfGW456L13_221 0.62 -0.2 259,454 + PfGW456L13_221 0.62 -0.5 259,455 - PfGW456L13_221 0.62 +1.3 259,500 + PfGW456L13_221 0.65 +1.7 259,500 + PfGW456L13_221 0.65 +0.6 259,500 + PfGW456L13_221 0.65 +0.8 259,500 + PfGW456L13_221 0.65 -0.2 259,500 + PfGW456L13_221 0.65 +0.5 259,501 - PfGW456L13_221 0.65 -0.2 259,501 - PfGW456L13_221 0.65 +1.1 259,602 + PfGW456L13_221 0.72 +0.7 259,602 + PfGW456L13_221 0.72 +0.5 259,602 + PfGW456L13_221 0.72 -0.6 259,602 + PfGW456L13_221 0.72 -1.6 259,602 + PfGW456L13_221 0.72 -1.0 259,602 + PfGW456L13_221 0.72 +0.8 259,602 + PfGW456L13_221 0.72 +1.4 259,602 + PfGW456L13_221 0.72 +0.4 259,602 + PfGW456L13_221 0.72 -0.2 259,602 + PfGW456L13_221 0.72 -0.4 259,602 + PfGW456L13_221 0.72 -0.9 259,602 + PfGW456L13_221 0.72 +0.6 259,602 + PfGW456L13_221 0.72 +2.2 259,602 + PfGW456L13_221 0.72 -1.7 259,602 + PfGW456L13_221 0.72 +0.1 259,602 + PfGW456L13_221 0.72 -0.5 259,602 + PfGW456L13_221 0.72 +0.5 259,602 + PfGW456L13_221 0.72 +1.3 259,602 + PfGW456L13_221 0.72 -0.2 259,602 + PfGW456L13_221 0.72 +1.2 259,603 - PfGW456L13_221 0.72 +2.3 259,603 - PfGW456L13_221 0.72 -1.3 259,603 - PfGW456L13_221 0.72 +0.0 259,603 - PfGW456L13_221 0.72 +0.1 259,603 - PfGW456L13_221 0.72 -0.1 259,603 - PfGW456L13_221 0.72 -1.4 259,603 - PfGW456L13_221 0.72 +0.4 259,603 - PfGW456L13_221 0.72 -2.6 259,603 - PfGW456L13_221 0.72 -0.7 259,603 - PfGW456L13_221 0.72 +1.2 259,603 - PfGW456L13_221 0.72 -0.9 259,603 - PfGW456L13_221 0.72 -0.3 259,603 - PfGW456L13_221 0.72 +0.8 259,603 - PfGW456L13_221 0.72 +0.2 259,603 - PfGW456L13_221 0.72 -1.1 259,603 - PfGW456L13_221 0.72 +1.1 259,684 - PfGW456L13_221 0.77 +0.2 259,762 - PfGW456L13_221 0.83 -2.8 259,815 + PfGW456L13_221 0.86 -0.1 259,815 + PfGW456L13_221 0.86 -0.1 259,815 + PfGW456L13_221 0.86 -0.8 259,815 + PfGW456L13_221 0.86 +0.7 259,815 + PfGW456L13_221 0.86 -0.8 259,815 + PfGW456L13_221 0.86 +0.7 259,815 + PfGW456L13_221 0.86 +1.0 259,815 + PfGW456L13_221 0.86 +1.2 259,815 + PfGW456L13_221 0.86 +0.7 259,815 + PfGW456L13_221 0.86 +0.4 259,815 + PfGW456L13_221 0.86 -0.0 259,816 - PfGW456L13_221 0.86 -2.0 259,816 - PfGW456L13_221 0.86 +0.2 259,816 - PfGW456L13_221 0.86 -1.5 259,816 - PfGW456L13_221 0.86 -0.3 259,816 - PfGW456L13_221 0.86 +0.4 259,816 - PfGW456L13_221 0.86 +0.6 259,816 - PfGW456L13_221 0.86 +1.0 259,816 - PfGW456L13_221 0.86 -0.0 259,816 - PfGW456L13_221 0.86 -1.6 259,917 + +0.2 259,980 + +0.3 259,980 + -2.0 259,980 + -0.3 259,981 - +0.4 259,981 - +0.6 259,981 - -0.3 260,012 + -0.1 260,013 - -0.9 260,013 - +1.0 260,021 + +0.2 260,021 + -0.5 260,021 + +0.4 260,022 - -0.4 260,022 - +1.9 260,022 - +0.4 260,026 + +1.3 260,026 + +0.1 260,045 + +0.2 260,045 + -0.8 260,045 + +0.5 260,045 + +1.3 260,045 + -0.1 260,046 - +1.2 260,046 - +0.4 260,046 - +1.2 260,046 - +2.2 260,049 + +0.0 260,049 + -0.1 260,050 - +1.8 260,050 - +1.8 260,081 + +0.4 260,081 + +1.4 260,082 - +1.5 260,082 - +0.4 260,082 - -0.4 260,116 - +0.9 260,116 - +1.2 260,116 - +2.0 260,116 - +2.2 260,116 - +0.7 260,194 - -0.2 260,194 - +1.1 260,194 - +1.5 260,209 + -1.0 260,210 - +0.4 260,234 + -0.1 260,313 + -0.3 260,354 + -0.9 260,355 - +0.2 260,376 + PfGW456L13_222 0.20 -1.0 260,599 + +2.4 260,599 + +1.5 260,600 - +1.0
Or see this region's nucleotide sequence