Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ypdI and yfdY are separated by 76 nucleotides yfdY and ddg are separated by 352 nucleotides ddg and ypdK are separated by 355 nucleotides ypdK and yfdZ are separated by 64 nucleotides
b2376: ypdI - predicted lipoprotein involved in colanic acid biosynthesis (NCBI), at 2,492,720 to 2,492,995
ypdI
b2377: yfdY - predicted inner membrane protein (NCBI), at 2,493,072 to 2,493,314
yfdY
b2378: ddg - putative heat shock protein (VIMSS), at 2,493,667 to 2,494,587
ddg
b4680: ypdK - expressed protein (RefSeq), at 2,494,943 to 2,495,014
ypdK
b2379: yfdZ - hypothetical protein (NCBI), at 2,495,079 to 2,496,317
yfdZ
Position (kb)
2493
2494
2495 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2492.687 kb on - strand at 2492.771 kb on - strand, within ypdI at 2492.771 kb on - strand, within ypdI at 2492.771 kb on - strand, within ypdI at 2492.771 kb on - strand, within ypdI at 2492.818 kb on - strand, within ypdI at 2492.818 kb on - strand, within ypdI at 2492.826 kb on - strand, within ypdI at 2492.828 kb on - strand, within ypdI at 2492.848 kb on + strand, within ypdI at 2492.855 kb on + strand, within ypdI at 2492.861 kb on - strand, within ypdI at 2492.865 kb on + strand, within ypdI at 2492.873 kb on - strand, within ypdI at 2492.873 kb on - strand, within ypdI at 2492.929 kb on - strand, within ypdI at 2492.929 kb on - strand, within ypdI at 2493.005 kb on + strand at 2493.043 kb on - strand at 2493.043 kb on - strand at 2493.043 kb on - strand at 2493.056 kb on + strand at 2493.061 kb on + strand at 2493.064 kb on - strand at 2493.064 kb on - strand at 2493.075 kb on - strand at 2493.075 kb on - strand at 2493.081 kb on + strand at 2493.081 kb on + strand at 2493.095 kb on - strand at 2493.159 kb on - strand, within yfdY at 2493.159 kb on - strand, within yfdY at 2493.220 kb on + strand, within yfdY at 2493.267 kb on - strand, within yfdY at 2493.267 kb on - strand, within yfdY at 2493.436 kb on + strand at 2493.551 kb on + strand at 2493.551 kb on + strand at 2493.568 kb on - strand at 2493.627 kb on - strand at 2493.627 kb on - strand at 2493.657 kb on - strand at 2493.667 kb on - strand at 2493.667 kb on - strand at 2493.762 kb on + strand, within ddg at 2493.833 kb on + strand, within ddg at 2493.833 kb on + strand, within ddg at 2493.878 kb on + strand, within ddg at 2493.924 kb on + strand, within ddg at 2493.936 kb on + strand, within ddg at 2493.965 kb on - strand, within ddg at 2494.277 kb on - strand, within ddg at 2494.430 kb on + strand, within ddg at 2494.446 kb on + strand, within ddg at 2494.446 kb on + strand, within ddg at 2494.522 kb on - strand at 2494.524 kb on + strand at 2494.527 kb on + strand at 2494.527 kb on + strand at 2494.527 kb on + strand at 2494.769 kb on + strand at 2494.795 kb on - strand at 2494.815 kb on - strand at 2494.883 kb on + strand at 2494.968 kb on + strand, within ypdK at 2495.161 kb on - strand at 2495.163 kb on - strand at 2495.163 kb on - strand at 2495.165 kb on + strand at 2495.358 kb on + strand, within yfdZ at 2495.371 kb on + strand, within yfdZ at 2495.404 kb on + strand, within yfdZ at 2495.412 kb on - strand, within yfdZ at 2495.412 kb on - strand, within yfdZ at 2495.444 kb on - strand, within yfdZ at 2495.444 kb on - strand, within yfdZ at 2495.480 kb on - strand, within yfdZ at 2495.480 kb on - strand, within yfdZ
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 2,492,687 - -0.6 2,492,771 - ypdI b2376 0.18 +0.1 2,492,771 - ypdI b2376 0.18 +0.4 2,492,771 - ypdI b2376 0.18 +0.2 2,492,771 - ypdI b2376 0.18 -0.9 2,492,818 - ypdI b2376 0.36 +1.4 2,492,818 - ypdI b2376 0.36 -0.6 2,492,826 - ypdI b2376 0.38 -0.1 2,492,828 - ypdI b2376 0.39 -0.5 2,492,848 + ypdI b2376 0.46 +1.3 2,492,855 + ypdI b2376 0.49 +0.9 2,492,861 - ypdI b2376 0.51 +0.7 2,492,865 + ypdI b2376 0.53 -0.7 2,492,873 - ypdI b2376 0.55 +0.6 2,492,873 - ypdI b2376 0.55 -0.7 2,492,929 - ypdI b2376 0.76 +0.4 2,492,929 - ypdI b2376 0.76 +0.0 2,493,005 + +0.5 2,493,043 - -2.5 2,493,043 - +0.1 2,493,043 - +0.5 2,493,056 + -0.4 2,493,061 + -2.5 2,493,064 - +0.3 2,493,064 - -0.5 2,493,075 - -0.1 2,493,075 - +0.2 2,493,081 + +0.5 2,493,081 + +0.9 2,493,095 - +0.7 2,493,159 - yfdY b2377 0.36 +1.0 2,493,159 - yfdY b2377 0.36 -0.3 2,493,220 + yfdY b2377 0.61 +0.1 2,493,267 - yfdY b2377 0.80 -0.1 2,493,267 - yfdY b2377 0.80 +1.9 2,493,436 + -1.1 2,493,551 + -0.5 2,493,551 + +0.3 2,493,568 - -0.4 2,493,627 - +0.2 2,493,627 - +0.6 2,493,657 - -0.1 2,493,667 - +2.2 2,493,667 - +0.6 2,493,762 + ddg b2378 0.10 -1.0 2,493,833 + ddg b2378 0.18 +0.2 2,493,833 + ddg b2378 0.18 +0.6 2,493,878 + ddg b2378 0.23 +0.2 2,493,924 + ddg b2378 0.28 -0.4 2,493,936 + ddg b2378 0.29 +0.7 2,493,965 - ddg b2378 0.32 +0.2 2,494,277 - ddg b2378 0.66 +1.9 2,494,430 + ddg b2378 0.83 +0.2 2,494,446 + ddg b2378 0.85 -0.1 2,494,446 + ddg b2378 0.85 -0.6 2,494,522 - +1.2 2,494,524 + +0.4 2,494,527 + +0.4 2,494,527 + +1.6 2,494,527 + -0.5 2,494,769 + +1.2 2,494,795 - +1.4 2,494,815 - -0.1 2,494,883 + +1.5 2,494,968 + ypdK b4680 0.35 -0.1 2,495,161 - +1.1 2,495,163 - +0.6 2,495,163 - -0.5 2,495,165 + +0.5 2,495,358 + yfdZ b2379 0.23 +1.6 2,495,371 + yfdZ b2379 0.24 -1.0 2,495,404 + yfdZ b2379 0.26 +0.5 2,495,412 - yfdZ b2379 0.27 +1.1 2,495,412 - yfdZ b2379 0.27 -0.7 2,495,444 - yfdZ b2379 0.29 -1.5 2,495,444 - yfdZ b2379 0.29 +1.0 2,495,480 - yfdZ b2379 0.32 +0.5 2,495,480 - yfdZ b2379 0.32 +0.9
Or see this region's nucleotide sequence