Experiment: m.b. D-Cycloserine 0.064 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Echvi_0257 and Echvi_0258 are separated by 5 nucleotides Echvi_0258 and Echvi_0259 are separated by 22 nucleotides Echvi_0259 and Echvi_0260 are separated by 99 nucleotides Echvi_0260 and Echvi_0261 are separated by 168 nucleotides
Echvi_0257: Echvi_0257 - translation elongation factor EF-G, at 259,598 to 261,718
_0257
Echvi_0258: Echvi_0258 - ribosomal protein S7, bacterial/organelle, at 261,724 to 262,191
_0258
Echvi_0259: Echvi_0259 - ribosomal protein S12, bacterial/organelle, at 262,214 to 262,594
_0259
Echvi_0260: Echvi_0260 - Protein of unknown function (DUF3467)., at 262,694 to 263,023
_0260
Echvi_0261: Echvi_0261 - DNA-directed RNA polymerase, beta' subunit, predominant form, at 263,192 to 267,505
_0261
Position (kb) 262
263 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 262.757 kb on + strand, within Echvi_0260 at 262.763 kb on - strand, within Echvi_0260 at 262.840 kb on + strand, within Echvi_0260 at 262.840 kb on + strand, within Echvi_0260 at 262.941 kb on + strand, within Echvi_0260 at 262.941 kb on + strand, within Echvi_0260 at 263.011 kb on + strand at 263.012 kb on - strand at 263.012 kb on - strand at 263.012 kb on - strand at 263.014 kb on + strand at 263.057 kb on - strand at 263.057 kb on - strand at 263.110 kb on + strand at 263.111 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. D-Cycloserine 0.064 mg/ml remove 262,757 + Echvi_0260 0.19 -1.1 262,763 - Echvi_0260 0.21 -0.1 262,840 + Echvi_0260 0.44 +2.5 262,840 + Echvi_0260 0.44 +1.2 262,941 + Echvi_0260 0.75 -0.3 262,941 + Echvi_0260 0.75 +1.6 263,011 + +1.2 263,012 - +1.3 263,012 - +1.3 263,012 - -3.2 263,014 + +2.3 263,057 - +2.3 263,057 - +1.3 263,110 + -0.5 263,111 - -0.7
Or see this region's nucleotide sequence