Experiment: m.b. D-Cycloserine 0.064 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Echvi_0005 and Echvi_0006 are separated by 15 nucleotides Echvi_0006 and Echvi_0007 overlap by 20 nucleotides Echvi_0007 and Echvi_0008 are separated by 13 nucleotides Echvi_0008 and Echvi_0009 are separated by 291 nucleotides
Echvi_0005: Echvi_0005 - Predicted membrane protein, at 4,135 to 4,671
_0005
Echvi_0006: Echvi_0006 - Uncharacterized protein SCO1/SenC/PrrC, involved in biogenesis of respiratory and photosynthetic systems, at 4,687 to 5,355
_0006
Echvi_0007: Echvi_0007 - Cytochrome c, mono- and diheme variants, at 5,336 to 5,800
_0007
Echvi_0008: Echvi_0008 - hypothetical protein, at 5,814 to 6,212
_0008
Echvi_0009: Echvi_0009 - Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase, at 6,504 to 7,325
_0009
Position (kb) 5
6 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 4.677 kb on + strand at 4.709 kb on + strand at 4.709 kb on + strand at 4.709 kb on + strand at 4.709 kb on + strand at 4.710 kb on - strand at 4.713 kb on + strand at 4.891 kb on - strand, within Echvi_0006 at 4.922 kb on + strand, within Echvi_0006 at 4.922 kb on + strand, within Echvi_0006 at 4.922 kb on + strand, within Echvi_0006 at 4.923 kb on - strand, within Echvi_0006 at 4.924 kb on + strand, within Echvi_0006 at 4.925 kb on - strand, within Echvi_0006 at 4.945 kb on - strand, within Echvi_0006 at 4.996 kb on + strand, within Echvi_0006 at 5.033 kb on + strand, within Echvi_0006 at 5.061 kb on + strand, within Echvi_0006 at 5.076 kb on - strand, within Echvi_0006 at 5.259 kb on + strand at 5.260 kb on - strand, within Echvi_0006 at 5.273 kb on - strand, within Echvi_0006 at 5.434 kb on + strand, within Echvi_0007 at 5.475 kb on - strand, within Echvi_0007 at 5.496 kb on + strand, within Echvi_0007 at 5.496 kb on + strand, within Echvi_0007 at 5.496 kb on + strand, within Echvi_0007 at 5.496 kb on + strand, within Echvi_0007 at 5.497 kb on - strand, within Echvi_0007 at 5.497 kb on - strand, within Echvi_0007 at 5.508 kb on - strand, within Echvi_0007 at 5.508 kb on - strand, within Echvi_0007 at 5.661 kb on - strand, within Echvi_0007 at 5.706 kb on + strand, within Echvi_0007 at 5.724 kb on - strand, within Echvi_0007 at 5.724 kb on - strand, within Echvi_0007 at 5.730 kb on - strand, within Echvi_0007 at 5.799 kb on - strand at 5.802 kb on + strand at 5.818 kb on + strand at 5.818 kb on + strand at 5.894 kb on - strand, within Echvi_0008 at 5.936 kb on + strand, within Echvi_0008 at 5.937 kb on - strand, within Echvi_0008 at 5.937 kb on - strand, within Echvi_0008 at 5.938 kb on + strand, within Echvi_0008 at 6.014 kb on + strand, within Echvi_0008 at 6.087 kb on + strand, within Echvi_0008 at 6.242 kb on + strand at 6.242 kb on + strand at 6.242 kb on + strand at 6.243 kb on - strand at 6.243 kb on - strand at 6.243 kb on - strand at 6.243 kb on - strand at 6.244 kb on + strand at 6.245 kb on - strand at 6.416 kb on + strand at 6.417 kb on - strand at 6.482 kb on - strand at 6.488 kb on - strand at 6.488 kb on - strand at 6.488 kb on - strand at 6.496 kb on + strand at 6.497 kb on - strand at 6.514 kb on - strand at 6.533 kb on + strand at 6.533 kb on + strand at 6.635 kb on - strand, within Echvi_0009 at 6.641 kb on - strand, within Echvi_0009 at 6.648 kb on + strand, within Echvi_0009 at 6.649 kb on - strand, within Echvi_0009 at 6.716 kb on + strand, within Echvi_0009 at 6.728 kb on - strand, within Echvi_0009 at 6.737 kb on + strand, within Echvi_0009 at 6.737 kb on + strand, within Echvi_0009 at 6.737 kb on + strand, within Echvi_0009
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. D-Cycloserine 0.064 mg/ml remove 4,677 + +0.9 4,709 + +1.1 4,709 + +0.0 4,709 + -0.1 4,709 + -0.1 4,710 - -3.8 4,713 + -0.1 4,891 - Echvi_0006 0.30 -1.0 4,922 + Echvi_0006 0.35 -1.0 4,922 + Echvi_0006 0.35 -0.5 4,922 + Echvi_0006 0.35 -2.1 4,923 - Echvi_0006 0.35 -1.6 4,924 + Echvi_0006 0.35 -0.5 4,925 - Echvi_0006 0.36 +1.3 4,945 - Echvi_0006 0.39 -2.5 4,996 + Echvi_0006 0.46 +0.3 5,033 + Echvi_0006 0.52 -2.1 5,061 + Echvi_0006 0.56 -1.6 5,076 - Echvi_0006 0.58 -0.3 5,259 + +2.3 5,260 - Echvi_0006 0.86 +0.2 5,273 - Echvi_0006 0.88 -0.7 5,434 + Echvi_0007 0.21 -1.6 5,475 - Echvi_0007 0.30 -1.5 5,496 + Echvi_0007 0.34 -0.4 5,496 + Echvi_0007 0.34 +1.0 5,496 + Echvi_0007 0.34 -1.1 5,496 + Echvi_0007 0.34 +0.0 5,497 - Echvi_0007 0.35 -1.6 5,497 - Echvi_0007 0.35 +1.9 5,508 - Echvi_0007 0.37 +1.3 5,508 - Echvi_0007 0.37 -0.7 5,661 - Echvi_0007 0.70 -2.0 5,706 + Echvi_0007 0.80 -2.2 5,724 - Echvi_0007 0.83 -1.3 5,724 - Echvi_0007 0.83 -1.1 5,730 - Echvi_0007 0.85 -1.8 5,799 - -1.2 5,802 + -1.2 5,818 + +1.2 5,818 + -1.3 5,894 - Echvi_0008 0.20 +0.9 5,936 + Echvi_0008 0.31 -1.0 5,937 - Echvi_0008 0.31 +0.0 5,937 - Echvi_0008 0.31 -0.8 5,938 + Echvi_0008 0.31 +1.2 6,014 + Echvi_0008 0.50 -0.0 6,087 + Echvi_0008 0.68 +1.2 6,242 + +1.1 6,242 + +0.7 6,242 + +0.8 6,243 - -0.8 6,243 - +1.3 6,243 - +0.1 6,243 - +1.5 6,244 + -2.6 6,245 - -0.1 6,416 + +1.6 6,417 - +0.4 6,482 - +0.3 6,488 - -0.4 6,488 - -1.0 6,488 - -0.8 6,496 + +0.7 6,497 - -2.3 6,514 - -0.6 6,533 + -0.7 6,533 + -2.3 6,635 - Echvi_0009 0.16 +0.6 6,641 - Echvi_0009 0.17 -1.8 6,648 + Echvi_0009 0.18 -1.7 6,649 - Echvi_0009 0.18 -2.3 6,716 + Echvi_0009 0.26 -1.6 6,728 - Echvi_0009 0.27 -2.0 6,737 + Echvi_0009 0.28 -1.8 6,737 + Echvi_0009 0.28 +0.3 6,737 + Echvi_0009 0.28 -1.9
Or see this region's nucleotide sequence