Experiment: m.b. D-Cycloserine 0.032 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Echvi_4565 and Echvi_4566 are separated by 91 nucleotides Echvi_4566 and Echvi_4567 are separated by 342 nucleotides Echvi_4567 and Echvi_4568 are separated by 12 nucleotides
Echvi_4565: Echvi_4565 - Fe2+-dicitrate sensor, membrane component, at 5,466,077 to 5,467,051
_4565
Echvi_4566: Echvi_4566 - RNA polymerase sigma-70 factor, Bacteroides expansion family 1, at 5,467,143 to 5,467,706
_4566
Echvi_4567: Echvi_4567 - Outer membrane receptor proteins, mostly Fe transport, at 5,468,049 to 5,470,421
_4567
Echvi_4568: Echvi_4568 - hypothetical protein, at 5,470,434 to 5,471,663
_4568
Position (kb)
5468
5469
5470
5471 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 5467.069 kb on + strand at 5467.099 kb on - strand at 5467.131 kb on - strand at 5467.264 kb on - strand, within Echvi_4566 at 5467.277 kb on + strand, within Echvi_4566 at 5467.278 kb on - strand, within Echvi_4566 at 5467.300 kb on + strand, within Echvi_4566 at 5467.301 kb on - strand, within Echvi_4566 at 5467.356 kb on + strand, within Echvi_4566 at 5467.367 kb on + strand, within Echvi_4566 at 5467.368 kb on - strand, within Echvi_4566 at 5467.400 kb on + strand, within Echvi_4566 at 5467.401 kb on - strand, within Echvi_4566 at 5467.401 kb on - strand, within Echvi_4566 at 5467.431 kb on + strand, within Echvi_4566 at 5467.431 kb on + strand, within Echvi_4566 at 5467.432 kb on - strand, within Echvi_4566 at 5467.509 kb on + strand, within Echvi_4566 at 5467.509 kb on + strand, within Echvi_4566 at 5467.510 kb on - strand, within Echvi_4566 at 5467.603 kb on + strand, within Echvi_4566 at 5467.604 kb on - strand, within Echvi_4566 at 5467.679 kb on - strand at 5467.716 kb on + strand at 5467.716 kb on + strand at 5467.716 kb on + strand at 5467.717 kb on - strand at 5467.717 kb on - strand at 5467.717 kb on - strand at 5467.846 kb on + strand at 5467.847 kb on - strand at 5467.935 kb on + strand at 5467.936 kb on - strand at 5468.021 kb on + strand at 5468.022 kb on - strand at 5468.028 kb on + strand at 5468.041 kb on - strand at 5468.048 kb on + strand at 5468.048 kb on + strand at 5468.058 kb on - strand at 5468.084 kb on - strand at 5468.096 kb on - strand at 5468.262 kb on + strand at 5468.263 kb on - strand at 5468.345 kb on + strand, within Echvi_4567 at 5468.345 kb on + strand, within Echvi_4567 at 5468.345 kb on + strand, within Echvi_4567 at 5468.346 kb on - strand, within Echvi_4567 at 5468.346 kb on - strand, within Echvi_4567 at 5468.346 kb on - strand, within Echvi_4567 at 5468.346 kb on - strand, within Echvi_4567 at 5468.346 kb on - strand, within Echvi_4567 at 5468.543 kb on + strand, within Echvi_4567 at 5468.543 kb on + strand, within Echvi_4567 at 5468.543 kb on + strand, within Echvi_4567 at 5468.543 kb on + strand, within Echvi_4567 at 5468.543 kb on + strand, within Echvi_4567 at 5468.544 kb on - strand, within Echvi_4567 at 5468.587 kb on + strand, within Echvi_4567 at 5468.642 kb on + strand, within Echvi_4567 at 5468.676 kb on + strand, within Echvi_4567 at 5468.676 kb on + strand, within Echvi_4567 at 5468.677 kb on - strand, within Echvi_4567 at 5468.678 kb on + strand, within Echvi_4567 at 5468.678 kb on + strand, within Echvi_4567 at 5468.760 kb on + strand, within Echvi_4567 at 5468.775 kb on + strand, within Echvi_4567 at 5468.776 kb on - strand, within Echvi_4567 at 5468.781 kb on + strand, within Echvi_4567 at 5468.781 kb on + strand, within Echvi_4567 at 5468.781 kb on + strand, within Echvi_4567 at 5468.823 kb on + strand, within Echvi_4567 at 5468.843 kb on + strand, within Echvi_4567 at 5468.843 kb on + strand, within Echvi_4567 at 5468.843 kb on + strand, within Echvi_4567 at 5468.844 kb on - strand, within Echvi_4567 at 5468.886 kb on + strand, within Echvi_4567 at 5468.888 kb on + strand, within Echvi_4567 at 5468.889 kb on - strand, within Echvi_4567 at 5468.889 kb on - strand, within Echvi_4567 at 5468.889 kb on - strand, within Echvi_4567 at 5468.928 kb on + strand, within Echvi_4567 at 5468.928 kb on + strand, within Echvi_4567 at 5469.057 kb on + strand, within Echvi_4567 at 5469.057 kb on + strand, within Echvi_4567 at 5469.059 kb on + strand, within Echvi_4567 at 5469.059 kb on + strand, within Echvi_4567 at 5469.144 kb on + strand, within Echvi_4567 at 5469.165 kb on - strand, within Echvi_4567 at 5469.198 kb on + strand, within Echvi_4567 at 5469.233 kb on - strand, within Echvi_4567 at 5469.270 kb on + strand, within Echvi_4567 at 5469.297 kb on + strand, within Echvi_4567 at 5469.298 kb on - strand, within Echvi_4567 at 5469.298 kb on - strand, within Echvi_4567 at 5469.307 kb on - strand, within Echvi_4567 at 5469.307 kb on - strand, within Echvi_4567 at 5469.307 kb on - strand, within Echvi_4567 at 5469.307 kb on - strand, within Echvi_4567 at 5469.323 kb on + strand, within Echvi_4567 at 5469.323 kb on + strand, within Echvi_4567 at 5469.324 kb on - strand, within Echvi_4567 at 5469.326 kb on + strand, within Echvi_4567 at 5469.326 kb on + strand, within Echvi_4567 at 5469.327 kb on - strand, within Echvi_4567 at 5469.368 kb on - strand, within Echvi_4567 at 5469.368 kb on - strand, within Echvi_4567 at 5469.368 kb on - strand, within Echvi_4567 at 5469.371 kb on + strand, within Echvi_4567 at 5469.371 kb on + strand, within Echvi_4567 at 5469.371 kb on + strand, within Echvi_4567 at 5469.371 kb on + strand, within Echvi_4567 at 5469.372 kb on - strand, within Echvi_4567 at 5469.372 kb on - strand, within Echvi_4567 at 5469.372 kb on - strand, within Echvi_4567 at 5469.372 kb on - strand, within Echvi_4567 at 5469.389 kb on + strand, within Echvi_4567 at 5469.389 kb on + strand, within Echvi_4567 at 5469.390 kb on - strand, within Echvi_4567 at 5469.395 kb on + strand, within Echvi_4567 at 5469.395 kb on + strand, within Echvi_4567 at 5469.558 kb on + strand, within Echvi_4567 at 5469.559 kb on - strand, within Echvi_4567 at 5469.573 kb on + strand, within Echvi_4567 at 5469.573 kb on + strand, within Echvi_4567 at 5469.573 kb on + strand, within Echvi_4567 at 5469.574 kb on - strand, within Echvi_4567 at 5469.574 kb on - strand, within Echvi_4567 at 5469.607 kb on - strand, within Echvi_4567 at 5469.661 kb on - strand, within Echvi_4567 at 5469.685 kb on + strand, within Echvi_4567 at 5469.708 kb on + strand, within Echvi_4567 at 5469.789 kb on + strand, within Echvi_4567 at 5469.789 kb on + strand, within Echvi_4567 at 5469.789 kb on + strand, within Echvi_4567 at 5469.790 kb on - strand, within Echvi_4567 at 5469.790 kb on - strand, within Echvi_4567 at 5469.790 kb on - strand, within Echvi_4567 at 5469.791 kb on + strand, within Echvi_4567 at 5469.795 kb on + strand, within Echvi_4567 at 5469.816 kb on - strand, within Echvi_4567 at 5470.108 kb on - strand, within Echvi_4567 at 5470.108 kb on - strand, within Echvi_4567 at 5470.134 kb on + strand, within Echvi_4567 at 5470.134 kb on + strand, within Echvi_4567 at 5470.136 kb on + strand, within Echvi_4567 at 5470.136 kb on + strand, within Echvi_4567 at 5470.137 kb on - strand, within Echvi_4567 at 5470.184 kb on + strand at 5470.185 kb on - strand at 5470.194 kb on + strand at 5470.194 kb on + strand at 5470.194 kb on + strand at 5470.194 kb on + strand at 5470.195 kb on - strand at 5470.195 kb on - strand at 5470.198 kb on + strand at 5470.199 kb on - strand at 5470.275 kb on - strand at 5470.284 kb on + strand at 5470.284 kb on + strand at 5470.285 kb on - strand at 5470.285 kb on - strand at 5470.285 kb on - strand at 5470.285 kb on - strand at 5470.286 kb on + strand at 5470.286 kb on + strand at 5470.286 kb on + strand at 5470.286 kb on + strand at 5470.287 kb on - strand at 5470.287 kb on - strand at 5470.287 kb on - strand at 5470.287 kb on - strand at 5470.347 kb on - strand at 5470.413 kb on - strand at 5470.417 kb on - strand at 5470.417 kb on - strand at 5470.419 kb on + strand at 5470.419 kb on + strand at 5470.419 kb on + strand at 5470.451 kb on + strand at 5470.500 kb on - strand at 5470.590 kb on + strand, within Echvi_4568 at 5470.614 kb on - strand, within Echvi_4568 at 5470.665 kb on + strand, within Echvi_4568 at 5470.666 kb on - strand, within Echvi_4568 at 5470.671 kb on + strand, within Echvi_4568 at 5470.674 kb on + strand, within Echvi_4568 at 5470.674 kb on + strand, within Echvi_4568 at 5470.675 kb on - strand, within Echvi_4568 at 5470.677 kb on - strand, within Echvi_4568 at 5470.677 kb on - strand, within Echvi_4568 at 5470.677 kb on - strand, within Echvi_4568 at 5470.677 kb on - strand, within Echvi_4568 at 5470.677 kb on - strand, within Echvi_4568 at 5470.677 kb on - strand, within Echvi_4568 at 5470.677 kb on - strand, within Echvi_4568 at 5470.679 kb on + strand, within Echvi_4568 at 5470.685 kb on + strand, within Echvi_4568 at 5470.743 kb on + strand, within Echvi_4568 at 5470.744 kb on - strand, within Echvi_4568 at 5470.996 kb on + strand, within Echvi_4568 at 5470.997 kb on - strand, within Echvi_4568 at 5470.997 kb on - strand, within Echvi_4568 at 5470.997 kb on - strand, within Echvi_4568 at 5471.007 kb on + strand, within Echvi_4568 at 5471.093 kb on + strand, within Echvi_4568 at 5471.115 kb on - strand, within Echvi_4568 at 5471.133 kb on + strand, within Echvi_4568 at 5471.133 kb on + strand, within Echvi_4568 at 5471.134 kb on - strand, within Echvi_4568 at 5471.134 kb on - strand, within Echvi_4568 at 5471.134 kb on - strand, within Echvi_4568 at 5471.135 kb on + strand, within Echvi_4568 at 5471.145 kb on + strand at 5471.187 kb on + strand, within Echvi_4568 at 5471.187 kb on + strand, within Echvi_4568 at 5471.187 kb on + strand, within Echvi_4568 at 5471.187 kb on + strand, within Echvi_4568 at 5471.188 kb on - strand, within Echvi_4568 at 5471.188 kb on - strand, within Echvi_4568 at 5471.346 kb on + strand, within Echvi_4568 at 5471.346 kb on + strand, within Echvi_4568 at 5471.347 kb on - strand, within Echvi_4568 at 5471.387 kb on - strand, within Echvi_4568 at 5471.397 kb on + strand, within Echvi_4568 at 5471.397 kb on + strand, within Echvi_4568 at 5471.397 kb on + strand, within Echvi_4568 at 5471.398 kb on - strand, within Echvi_4568 at 5471.400 kb on - strand, within Echvi_4568 at 5471.411 kb on + strand, within Echvi_4568 at 5471.412 kb on - strand, within Echvi_4568 at 5471.412 kb on - strand, within Echvi_4568
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. D-Cycloserine 0.032 mg/ml remove 5,467,069 + +0.2 5,467,099 - +2.6 5,467,131 - -0.8 5,467,264 - Echvi_4566 0.21 +0.1 5,467,277 + Echvi_4566 0.24 -1.2 5,467,278 - Echvi_4566 0.24 +0.7 5,467,300 + Echvi_4566 0.28 -0.1 5,467,301 - Echvi_4566 0.28 +0.3 5,467,356 + Echvi_4566 0.38 +0.0 5,467,367 + Echvi_4566 0.40 -0.0 5,467,368 - Echvi_4566 0.40 +0.2 5,467,400 + Echvi_4566 0.46 -1.6 5,467,401 - Echvi_4566 0.46 +0.4 5,467,401 - Echvi_4566 0.46 -0.1 5,467,431 + Echvi_4566 0.51 -0.2 5,467,431 + Echvi_4566 0.51 -0.3 5,467,432 - Echvi_4566 0.51 +0.6 5,467,509 + Echvi_4566 0.65 +1.2 5,467,509 + Echvi_4566 0.65 -0.8 5,467,510 - Echvi_4566 0.65 -0.1 5,467,603 + Echvi_4566 0.82 -0.2 5,467,604 - Echvi_4566 0.82 -0.3 5,467,679 - -0.1 5,467,716 + -0.7 5,467,716 + -2.9 5,467,716 + +0.4 5,467,717 - +0.5 5,467,717 - +1.1 5,467,717 - +1.2 5,467,846 + -0.5 5,467,847 - +0.4 5,467,935 + -0.8 5,467,936 - -1.1 5,468,021 + -1.3 5,468,022 - +0.4 5,468,028 + -1.4 5,468,041 - -1.2 5,468,048 + +0.4 5,468,048 + +0.0 5,468,058 - +0.0 5,468,084 - -2.1 5,468,096 - -0.0 5,468,262 + -2.8 5,468,263 - -0.2 5,468,345 + Echvi_4567 0.12 -0.2 5,468,345 + Echvi_4567 0.12 -1.2 5,468,345 + Echvi_4567 0.12 -0.3 5,468,346 - Echvi_4567 0.13 -0.5 5,468,346 - Echvi_4567 0.13 +1.1 5,468,346 - Echvi_4567 0.13 -0.4 5,468,346 - Echvi_4567 0.13 +1.0 5,468,346 - Echvi_4567 0.13 +0.6 5,468,543 + Echvi_4567 0.21 +0.1 5,468,543 + Echvi_4567 0.21 -0.4 5,468,543 + Echvi_4567 0.21 -0.6 5,468,543 + Echvi_4567 0.21 -1.1 5,468,543 + Echvi_4567 0.21 +0.2 5,468,544 - Echvi_4567 0.21 -0.4 5,468,587 + Echvi_4567 0.23 -1.0 5,468,642 + Echvi_4567 0.25 -0.9 5,468,676 + Echvi_4567 0.26 +0.3 5,468,676 + Echvi_4567 0.26 -0.1 5,468,677 - Echvi_4567 0.26 +1.2 5,468,678 + Echvi_4567 0.27 +0.3 5,468,678 + Echvi_4567 0.27 -2.2 5,468,760 + Echvi_4567 0.30 -0.2 5,468,775 + Echvi_4567 0.31 -0.7 5,468,776 - Echvi_4567 0.31 +0.8 5,468,781 + Echvi_4567 0.31 +0.7 5,468,781 + Echvi_4567 0.31 +0.9 5,468,781 + Echvi_4567 0.31 +0.2 5,468,823 + Echvi_4567 0.33 -0.6 5,468,843 + Echvi_4567 0.33 -0.7 5,468,843 + Echvi_4567 0.33 -0.5 5,468,843 + Echvi_4567 0.33 -1.0 5,468,844 - Echvi_4567 0.34 -0.6 5,468,886 + Echvi_4567 0.35 -0.7 5,468,888 + Echvi_4567 0.35 -1.0 5,468,889 - Echvi_4567 0.35 +1.0 5,468,889 - Echvi_4567 0.35 -0.3 5,468,889 - Echvi_4567 0.35 -0.3 5,468,928 + Echvi_4567 0.37 +1.2 5,468,928 + Echvi_4567 0.37 -0.9 5,469,057 + Echvi_4567 0.42 -2.1 5,469,057 + Echvi_4567 0.42 -0.6 5,469,059 + Echvi_4567 0.43 -0.1 5,469,059 + Echvi_4567 0.43 -0.5 5,469,144 + Echvi_4567 0.46 -0.4 5,469,165 - Echvi_4567 0.47 +0.7 5,469,198 + Echvi_4567 0.48 -1.1 5,469,233 - Echvi_4567 0.50 -0.1 5,469,270 + Echvi_4567 0.51 +0.2 5,469,297 + Echvi_4567 0.53 -0.2 5,469,298 - Echvi_4567 0.53 +0.1 5,469,298 - Echvi_4567 0.53 +0.8 5,469,307 - Echvi_4567 0.53 +0.2 5,469,307 - Echvi_4567 0.53 +0.2 5,469,307 - Echvi_4567 0.53 +1.1 5,469,307 - Echvi_4567 0.53 -1.7 5,469,323 + Echvi_4567 0.54 -0.2 5,469,323 + Echvi_4567 0.54 -0.5 5,469,324 - Echvi_4567 0.54 -0.6 5,469,326 + Echvi_4567 0.54 -0.1 5,469,326 + Echvi_4567 0.54 -0.8 5,469,327 - Echvi_4567 0.54 -0.1 5,469,368 - Echvi_4567 0.56 +0.4 5,469,368 - Echvi_4567 0.56 -1.0 5,469,368 - Echvi_4567 0.56 -0.1 5,469,371 + Echvi_4567 0.56 -0.2 5,469,371 + Echvi_4567 0.56 -0.8 5,469,371 + Echvi_4567 0.56 -2.4 5,469,371 + Echvi_4567 0.56 +0.2 5,469,372 - Echvi_4567 0.56 +0.5 5,469,372 - Echvi_4567 0.56 -0.3 5,469,372 - Echvi_4567 0.56 +0.8 5,469,372 - Echvi_4567 0.56 -0.2 5,469,389 + Echvi_4567 0.56 +0.5 5,469,389 + Echvi_4567 0.56 -1.4 5,469,390 - Echvi_4567 0.57 -0.6 5,469,395 + Echvi_4567 0.57 -1.4 5,469,395 + Echvi_4567 0.57 -1.4 5,469,558 + Echvi_4567 0.64 -0.7 5,469,559 - Echvi_4567 0.64 -0.2 5,469,573 + Echvi_4567 0.64 -1.1 5,469,573 + Echvi_4567 0.64 -1.1 5,469,573 + Echvi_4567 0.64 +1.0 5,469,574 - Echvi_4567 0.64 -0.2 5,469,574 - Echvi_4567 0.64 +1.2 5,469,607 - Echvi_4567 0.66 -0.5 5,469,661 - Echvi_4567 0.68 -0.3 5,469,685 + Echvi_4567 0.69 -2.4 5,469,708 + Echvi_4567 0.70 -0.3 5,469,789 + Echvi_4567 0.73 -1.2 5,469,789 + Echvi_4567 0.73 -0.4 5,469,789 + Echvi_4567 0.73 -0.3 5,469,790 - Echvi_4567 0.73 -0.5 5,469,790 - Echvi_4567 0.73 +1.6 5,469,790 - Echvi_4567 0.73 +0.2 5,469,791 + Echvi_4567 0.73 -0.9 5,469,795 + Echvi_4567 0.74 +0.1 5,469,816 - Echvi_4567 0.74 -0.6 5,470,108 - Echvi_4567 0.87 -0.3 5,470,108 - Echvi_4567 0.87 -0.0 5,470,134 + Echvi_4567 0.88 -1.2 5,470,134 + Echvi_4567 0.88 -0.2 5,470,136 + Echvi_4567 0.88 -1.0 5,470,136 + Echvi_4567 0.88 -0.2 5,470,137 - Echvi_4567 0.88 -0.2 5,470,184 + -2.4 5,470,185 - -0.6 5,470,194 + +0.2 5,470,194 + -1.7 5,470,194 + +0.1 5,470,194 + -2.0 5,470,195 - +0.9 5,470,195 - -0.2 5,470,198 + -3.0 5,470,199 - -0.2 5,470,275 - -0.1 5,470,284 + +0.3 5,470,284 + +0.2 5,470,285 - -1.6 5,470,285 - +0.0 5,470,285 - -0.2 5,470,285 - -1.1 5,470,286 + -0.6 5,470,286 + -0.5 5,470,286 + -1.7 5,470,286 + -1.7 5,470,287 - -1.2 5,470,287 - -0.4 5,470,287 - +0.1 5,470,287 - -0.2 5,470,347 - -0.1 5,470,413 - +2.3 5,470,417 - -0.8 5,470,417 - -0.0 5,470,419 + -0.6 5,470,419 + -0.6 5,470,419 + +0.8 5,470,451 + +2.1 5,470,500 - +0.2 5,470,590 + Echvi_4568 0.13 -0.4 5,470,614 - Echvi_4568 0.15 +0.5 5,470,665 + Echvi_4568 0.19 +0.4 5,470,666 - Echvi_4568 0.19 +1.0 5,470,671 + Echvi_4568 0.19 +0.5 5,470,674 + Echvi_4568 0.20 +0.8 5,470,674 + Echvi_4568 0.20 -0.3 5,470,675 - Echvi_4568 0.20 -0.7 5,470,677 - Echvi_4568 0.20 +0.6 5,470,677 - Echvi_4568 0.20 +0.6 5,470,677 - Echvi_4568 0.20 -0.4 5,470,677 - Echvi_4568 0.20 -1.1 5,470,677 - Echvi_4568 0.20 -0.2 5,470,677 - Echvi_4568 0.20 +0.8 5,470,677 - Echvi_4568 0.20 -0.4 5,470,679 + Echvi_4568 0.20 -0.1 5,470,685 + Echvi_4568 0.20 +0.4 5,470,743 + Echvi_4568 0.25 +0.1 5,470,744 - Echvi_4568 0.25 -0.5 5,470,996 + Echvi_4568 0.46 +0.6 5,470,997 - Echvi_4568 0.46 +1.1 5,470,997 - Echvi_4568 0.46 +0.2 5,470,997 - Echvi_4568 0.46 +1.4 5,471,007 + Echvi_4568 0.47 +0.5 5,471,093 + Echvi_4568 0.54 +0.1 5,471,115 - Echvi_4568 0.55 +0.7 5,471,133 + Echvi_4568 0.57 +0.2 5,471,133 + Echvi_4568 0.57 +0.8 5,471,134 - Echvi_4568 0.57 -1.6 5,471,134 - Echvi_4568 0.57 -0.6 5,471,134 - Echvi_4568 0.57 +0.2 5,471,135 + Echvi_4568 0.57 -0.2 5,471,145 + +1.8 5,471,187 + Echvi_4568 0.61 +0.5 5,471,187 + Echvi_4568 0.61 +0.9 5,471,187 + Echvi_4568 0.61 +0.2 5,471,187 + Echvi_4568 0.61 +0.5 5,471,188 - Echvi_4568 0.61 +0.7 5,471,188 - Echvi_4568 0.61 -0.3 5,471,346 + Echvi_4568 0.74 -1.1 5,471,346 + Echvi_4568 0.74 -0.8 5,471,347 - Echvi_4568 0.74 -0.5 5,471,387 - Echvi_4568 0.77 -0.6 5,471,397 + Echvi_4568 0.78 -0.9 5,471,397 + Echvi_4568 0.78 +0.4 5,471,397 + Echvi_4568 0.78 -0.1 5,471,398 - Echvi_4568 0.78 -0.7 5,471,400 - Echvi_4568 0.79 -2.2 5,471,411 + Echvi_4568 0.79 +0.3 5,471,412 - Echvi_4568 0.80 +0.6 5,471,412 - Echvi_4568 0.80 +0.2
Or see this region's nucleotide sequence