Experiment: Carbenicillin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yafT and ykfM are separated by 136 nucleotides ykfM and yafU are separated by 9 nucleotides yafU and yafF are separated by 3 nucleotides yafF and yafV are separated by 40 nucleotides yafV and ivy are separated by 153 nucleotides
b0217: yafT - predicted aminopeptidase (NCBI), at 237,335 to 238,120
yafT
b4586: ykfM - hypothetical protein, no homologs (RefSeq), at 238,257 to 238,736
ykfM
b0218: yafU - predicted inner membrane protein (RefSeq), at 238,746 to 239,102
yafU
b4503: yafF - no description, at 239,106 to 239,378
yafF
b0219: yafV - predicted C-N hydrolase family amidase, NAD(P)-binding (NCBI), at 239,419 to 240,189
yafV
b0220: ivy - inhibitor of vertebrate C-lysozyme (NCBI), at 240,343 to 240,816
ivy
Position (kb) 239
240 Strain fitness (log2 ratio) -1
0
1 at 238.734 kb on - strand at 238.901 kb on + strand, within yafU at 238.910 kb on - strand, within yafU at 238.915 kb on - strand, within yafU at 238.915 kb on - strand, within yafU at 238.915 kb on - strand, within yafU at 239.013 kb on + strand, within yafU at 239.013 kb on + strand, within yafU at 239.013 kb on + strand, within yafU at 239.036 kb on - strand, within yafU at 239.036 kb on - strand, within yafU at 239.163 kb on + strand, within yafF at 239.166 kb on + strand, within yafF at 239.392 kb on - strand at 239.412 kb on + strand at 239.414 kb on - strand at 239.417 kb on + strand at 239.426 kb on + strand at 239.436 kb on - strand at 239.436 kb on - strand at 239.850 kb on - strand, within yafV at 239.850 kb on - strand, within yafV at 239.907 kb on + strand, within yafV at 240.066 kb on + strand, within yafV at 240.072 kb on + strand, within yafV at 240.098 kb on - strand, within yafV at 240.145 kb on + strand at 240.148 kb on + strand at 240.175 kb on + strand at 240.197 kb on - strand at 240.197 kb on - strand at 240.199 kb on - strand at 240.199 kb on - strand at 240.224 kb on + strand at 240.261 kb on - strand at 240.325 kb on + strand at 240.325 kb on + strand at 240.333 kb on + strand at 240.350 kb on + strand at 240.350 kb on + strand at 240.358 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Carbenicillin 0.008 mg/ml remove 238,734 - +0.2 238,901 + yafU b0218 0.43 +0.2 238,910 - yafU b0218 0.46 +0.1 238,915 - yafU b0218 0.47 -1.5 238,915 - yafU b0218 0.47 +0.6 238,915 - yafU b0218 0.47 +0.2 239,013 + yafU b0218 0.75 -1.0 239,013 + yafU b0218 0.75 -1.0 239,013 + yafU b0218 0.75 -0.1 239,036 - yafU b0218 0.81 +1.0 239,036 - yafU b0218 0.81 +0.7 239,163 + yafF b4503 0.21 -0.6 239,166 + yafF b4503 0.22 -0.7 239,392 - -0.2 239,412 + +0.5 239,414 - +0.4 239,417 + +1.3 239,426 + +0.9 239,436 - -0.8 239,436 - +0.2 239,850 - yafV b0219 0.56 -0.0 239,850 - yafV b0219 0.56 -0.0 239,907 + yafV b0219 0.63 -0.2 240,066 + yafV b0219 0.84 +0.1 240,072 + yafV b0219 0.85 -0.2 240,098 - yafV b0219 0.88 -0.6 240,145 + -0.8 240,148 + +0.2 240,175 + +0.4 240,197 - -0.0 240,197 - +0.4 240,199 - +1.7 240,199 - +0.4 240,224 + +0.2 240,261 - +1.0 240,325 + +0.7 240,325 + +0.5 240,333 + +1.4 240,350 + -0.2 240,350 + +0.4 240,358 - +1.0
Or see this region's nucleotide sequence