Experiment: Carbenicillin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ccmB and ccmA overlap by 4 nucleotides ccmA and napC are separated by 12 nucleotides napC and napB are separated by 9 nucleotides napB and napH overlap by 4 nucleotides
b2200: ccmB - heme exporter subunit (NCBI), at 2,294,384 to 2,295,046
ccmB
b2201: ccmA - ATP binding protein of heme exporter A (VIMSS), at 2,295,043 to 2,295,666
ccmA
b2202: napC - nitrate reductase, cytochrome c-type, periplasmic (NCBI), at 2,295,679 to 2,296,281
napC
b2203: napB - cytochrome c-type protein (VIMSS), at 2,296,291 to 2,296,740
napB
b2204: napH - quinol dehydrogenase membrane component (NCBI), at 2,296,737 to 2,297,600
napH
Position (kb)
2295
2296
2297 Strain fitness (log2 ratio)
-1
0
1
2
3 at 2294.744 kb on - strand, within ccmB at 2294.744 kb on - strand, within ccmB at 2294.904 kb on - strand, within ccmB at 2294.928 kb on - strand, within ccmB at 2294.999 kb on + strand at 2295.133 kb on + strand, within ccmA at 2295.133 kb on + strand, within ccmA at 2295.152 kb on + strand, within ccmA at 2295.207 kb on + strand, within ccmA at 2295.207 kb on + strand, within ccmA at 2295.285 kb on + strand, within ccmA at 2295.401 kb on + strand, within ccmA at 2295.433 kb on - strand, within ccmA at 2295.476 kb on + strand, within ccmA at 2295.476 kb on + strand, within ccmA at 2295.516 kb on - strand, within ccmA at 2295.575 kb on - strand, within ccmA at 2295.650 kb on + strand at 2295.690 kb on + strand at 2295.722 kb on - strand at 2295.760 kb on + strand, within napC at 2295.760 kb on + strand, within napC at 2295.769 kb on + strand, within napC at 2295.806 kb on + strand, within napC at 2295.880 kb on + strand, within napC at 2295.921 kb on - strand, within napC at 2296.069 kb on - strand, within napC at 2296.154 kb on - strand, within napC at 2296.254 kb on + strand at 2296.254 kb on + strand at 2296.348 kb on + strand, within napB at 2296.348 kb on + strand, within napB at 2296.356 kb on + strand, within napB at 2296.356 kb on + strand, within napB at 2296.368 kb on + strand, within napB at 2296.431 kb on + strand, within napB at 2296.491 kb on + strand, within napB at 2296.491 kb on + strand, within napB at 2296.491 kb on - strand, within napB at 2296.545 kb on + strand, within napB at 2296.578 kb on + strand, within napB at 2296.578 kb on + strand, within napB at 2296.675 kb on + strand, within napB at 2296.695 kb on - strand, within napB at 2296.773 kb on + strand at 2296.773 kb on + strand at 2296.779 kb on - strand at 2296.880 kb on - strand, within napH at 2296.953 kb on + strand, within napH at 2297.095 kb on - strand, within napH
Per-strain Table
Position Strand Gene LocusTag Fraction Carbenicillin 0.008 mg/ml remove 2,294,744 - ccmB b2200 0.54 -0.2 2,294,744 - ccmB b2200 0.54 -0.9 2,294,904 - ccmB b2200 0.78 -0.0 2,294,928 - ccmB b2200 0.82 -0.4 2,294,999 + +2.4 2,295,133 + ccmA b2201 0.14 -0.3 2,295,133 + ccmA b2201 0.14 +1.2 2,295,152 + ccmA b2201 0.17 +0.9 2,295,207 + ccmA b2201 0.26 -1.2 2,295,207 + ccmA b2201 0.26 +0.5 2,295,285 + ccmA b2201 0.39 +0.0 2,295,401 + ccmA b2201 0.57 +1.3 2,295,433 - ccmA b2201 0.62 -0.6 2,295,476 + ccmA b2201 0.69 +1.2 2,295,476 + ccmA b2201 0.69 -1.6 2,295,516 - ccmA b2201 0.76 +0.9 2,295,575 - ccmA b2201 0.85 +0.1 2,295,650 + +1.4 2,295,690 + +1.7 2,295,722 - +0.3 2,295,760 + napC b2202 0.13 -0.6 2,295,760 + napC b2202 0.13 +1.2 2,295,769 + napC b2202 0.15 +0.9 2,295,806 + napC b2202 0.21 -0.6 2,295,880 + napC b2202 0.33 -1.4 2,295,921 - napC b2202 0.40 +0.7 2,296,069 - napC b2202 0.65 +1.2 2,296,154 - napC b2202 0.79 -0.5 2,296,254 + +1.3 2,296,254 + -0.1 2,296,348 + napB b2203 0.13 +0.3 2,296,348 + napB b2203 0.13 +0.3 2,296,356 + napB b2203 0.14 +0.4 2,296,356 + napB b2203 0.14 +0.4 2,296,368 + napB b2203 0.17 -1.6 2,296,431 + napB b2203 0.31 +0.9 2,296,491 + napB b2203 0.44 +0.9 2,296,491 + napB b2203 0.44 +0.1 2,296,491 - napB b2203 0.44 +0.6 2,296,545 + napB b2203 0.56 +1.7 2,296,578 + napB b2203 0.64 -0.6 2,296,578 + napB b2203 0.64 -1.6 2,296,675 + napB b2203 0.85 +0.9 2,296,695 - napB b2203 0.90 -1.4 2,296,773 + +2.5 2,296,773 + +1.1 2,296,779 - +2.3 2,296,880 - napH b2204 0.17 +3.3 2,296,953 + napH b2204 0.25 +0.4 2,297,095 - napH b2204 0.41 +0.9
Or see this region's nucleotide sequence