Experiment: Carbenicillin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt paaA and paaB are separated by 11 nucleotides paaB and paaC are separated by 8 nucleotides paaC and paaD are separated by 14 nucleotides paaD and paaE are separated by 7 nucleotides
b1388: paaA - predicted multicomponent oxygenase/reductase subunit for phenylacetic acid degradation (NCBI), at 1,451,951 to 1,452,880
paaA
b1389: paaB - predicted multicomponent oxygenase/reductase subunit for phenylacetic acid degradation (NCBI), at 1,452,892 to 1,453,179
paaB
b1390: paaC - predicted multicomponent oxygenase/reductase subunit for phenylacetic acid degradation (NCBI), at 1,453,188 to 1,453,934
paaC
b1391: paaD - predicted multicomponent oxygenase/reductase subunit for phenylacetic acid degradation (RefSeq), at 1,453,949 to 1,454,446
paaD
b1392: paaE - predicted multicomponent oxygenase/reductase subunit for phenylacetic acid degradation (NCBI), at 1,454,454 to 1,455,524
paaE
Position (kb)
1453
1454 Strain fitness (log2 ratio)
-1
0
1 at 1452.190 kb on - strand, within paaA at 1452.425 kb on - strand, within paaA at 1452.529 kb on - strand, within paaA at 1452.529 kb on - strand, within paaA at 1452.655 kb on + strand, within paaA at 1453.221 kb on + strand at 1453.221 kb on + strand at 1453.483 kb on - strand, within paaC at 1453.635 kb on - strand, within paaC at 1453.641 kb on + strand, within paaC at 1453.689 kb on + strand, within paaC at 1453.840 kb on - strand, within paaC at 1454.381 kb on + strand, within paaD at 1454.381 kb on + strand, within paaD at 1454.398 kb on - strand at 1454.470 kb on + strand at 1454.532 kb on - strand at 1454.714 kb on + strand, within paaE at 1454.714 kb on + strand, within paaE at 1454.786 kb on - strand, within paaE
Per-strain Table
Position Strand Gene LocusTag Fraction Carbenicillin 0.008 mg/ml remove 1,452,190 - paaA b1388 0.26 -1.6 1,452,425 - paaA b1388 0.51 -0.3 1,452,529 - paaA b1388 0.62 -0.2 1,452,529 - paaA b1388 0.62 +0.6 1,452,655 + paaA b1388 0.76 +0.6 1,453,221 + +0.6 1,453,221 + +0.8 1,453,483 - paaC b1390 0.39 -1.2 1,453,635 - paaC b1390 0.60 -1.3 1,453,641 + paaC b1390 0.61 -0.2 1,453,689 + paaC b1390 0.67 +0.1 1,453,840 - paaC b1390 0.87 -1.1 1,454,381 + paaD b1391 0.87 -0.2 1,454,381 + paaD b1391 0.87 -0.7 1,454,398 - +0.3 1,454,470 + +0.5 1,454,532 - -1.2 1,454,714 + paaE b1392 0.24 +0.1 1,454,714 + paaE b1392 0.24 -0.0 1,454,786 - paaE b1392 0.31 -1.2
Or see this region's nucleotide sequence