Experiment: Carbenicillin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hdfR and yifE are separated by 118 nucleotides yifE and yifB are separated by 24 nucleotides yifB and ilvL are separated by 352 nucleotides ilvL and ilvX are separated by 86 nucleotides ilvX and ilvG are separated by 2 nucleotides
b4480: hdfR - transcriptional regulator HdfR (NCBI), at 3,945,151 to 3,945,990
hdfR
b3764: yifE - hypothetical protein (NCBI), at 3,946,109 to 3,946,447
yifE
b3765: yifB - predicted bifunctional enzyme and transcriptional regulator (RefSeq), at 3,946,472 to 3,947,992
yifB
b3766: ilvL - ilvG operon leader peptide (NCBI), at 3,948,345 to 3,948,443
ilvL
b4669: ilvX - expressed protein (RefSeq), at 3,948,530 to 3,948,580
ilvX
b4488: ilvG - no description, at 3,948,583 to 3,950,227
ilvG
Position (kb)
3946
3947
3948 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3945.543 kb on + strand, within hdfR at 3945.543 kb on + strand, within hdfR at 3945.551 kb on - strand, within hdfR at 3945.744 kb on - strand, within hdfR at 3945.783 kb on + strand, within hdfR at 3945.783 kb on + strand, within hdfR at 3945.897 kb on + strand, within hdfR at 3945.902 kb on + strand, within hdfR at 3945.914 kb on - strand at 3946.030 kb on - strand at 3946.030 kb on - strand at 3946.045 kb on + strand at 3946.056 kb on + strand at 3946.058 kb on + strand at 3946.064 kb on - strand at 3946.071 kb on + strand at 3946.102 kb on + strand at 3946.102 kb on + strand at 3946.127 kb on - strand at 3946.176 kb on - strand, within yifE at 3946.179 kb on - strand, within yifE at 3946.200 kb on + strand, within yifE at 3946.281 kb on - strand, within yifE at 3946.281 kb on - strand, within yifE at 3946.295 kb on - strand, within yifE at 3946.309 kb on + strand, within yifE at 3946.345 kb on + strand, within yifE at 3946.360 kb on + strand, within yifE at 3946.360 kb on + strand, within yifE at 3946.366 kb on - strand, within yifE at 3946.366 kb on - strand, within yifE at 3946.525 kb on + strand at 3946.528 kb on - strand at 3946.528 kb on - strand at 3946.575 kb on - strand at 3946.575 kb on - strand at 3946.621 kb on - strand at 3946.621 kb on - strand at 3946.628 kb on + strand, within yifB at 3946.632 kb on + strand, within yifB at 3946.706 kb on + strand, within yifB at 3946.761 kb on + strand, within yifB at 3946.883 kb on - strand, within yifB at 3946.903 kb on - strand, within yifB at 3947.019 kb on + strand, within yifB at 3947.019 kb on + strand, within yifB at 3947.020 kb on - strand, within yifB at 3947.047 kb on + strand, within yifB at 3947.047 kb on + strand, within yifB at 3947.065 kb on - strand, within yifB at 3947.065 kb on - strand, within yifB at 3947.070 kb on - strand, within yifB at 3947.177 kb on + strand, within yifB at 3947.178 kb on - strand, within yifB at 3947.178 kb on - strand, within yifB at 3947.180 kb on + strand, within yifB at 3947.180 kb on + strand, within yifB at 3947.195 kb on - strand, within yifB at 3947.240 kb on - strand, within yifB at 3947.251 kb on - strand, within yifB at 3947.251 kb on - strand, within yifB at 3947.269 kb on - strand, within yifB at 3947.269 kb on - strand, within yifB at 3947.339 kb on - strand, within yifB at 3947.409 kb on - strand, within yifB at 3947.423 kb on - strand, within yifB at 3947.423 kb on - strand, within yifB at 3947.423 kb on - strand, within yifB at 3947.429 kb on + strand, within yifB at 3947.429 kb on + strand, within yifB at 3947.437 kb on - strand, within yifB at 3947.449 kb on - strand, within yifB at 3947.449 kb on - strand, within yifB at 3947.479 kb on - strand, within yifB at 3947.481 kb on + strand, within yifB at 3947.506 kb on - strand, within yifB at 3947.506 kb on - strand, within yifB at 3947.523 kb on + strand, within yifB at 3947.523 kb on + strand, within yifB at 3947.532 kb on - strand, within yifB at 3947.543 kb on + strand, within yifB at 3947.543 kb on + strand, within yifB at 3947.746 kb on + strand, within yifB at 3947.833 kb on - strand, within yifB at 3947.836 kb on - strand, within yifB at 3947.841 kb on + strand at 3947.903 kb on + strand at 3947.903 kb on + strand at 3947.984 kb on - strand at 3948.303 kb on + strand at 3948.305 kb on + strand at 3948.364 kb on + strand, within ilvL at 3948.375 kb on + strand, within ilvL at 3948.383 kb on - strand, within ilvL at 3948.410 kb on + strand, within ilvL at 3948.410 kb on + strand, within ilvL at 3948.410 kb on + strand, within ilvL at 3948.531 kb on - strand at 3948.579 kb on + strand at 3948.580 kb on + strand at 3948.580 kb on + strand at 3948.604 kb on + strand at 3948.604 kb on + strand at 3948.620 kb on + strand at 3948.620 kb on + strand at 3948.624 kb on + strand at 3948.633 kb on - strand at 3948.633 kb on - strand at 3948.633 kb on - strand at 3948.682 kb on + strand at 3948.721 kb on + strand at 3948.726 kb on + strand at 3948.748 kb on + strand, within ilvG at 3948.748 kb on + strand, within ilvG at 3948.781 kb on + strand, within ilvG at 3948.815 kb on + strand, within ilvG at 3948.815 kb on + strand, within ilvG at 3948.851 kb on + strand, within ilvG at 3948.851 kb on + strand, within ilvG at 3948.851 kb on + strand, within ilvG at 3948.859 kb on - strand, within ilvG at 3948.933 kb on + strand, within ilvG at 3948.939 kb on + strand, within ilvG at 3948.944 kb on + strand, within ilvG at 3948.947 kb on - strand, within ilvG at 3948.947 kb on - strand, within ilvG at 3948.966 kb on - strand, within ilvG
Per-strain Table
Position Strand Gene LocusTag Fraction Carbenicillin 0.004 mg/ml remove 3,945,543 + hdfR b4480 0.47 -1.1 3,945,543 + hdfR b4480 0.47 +0.7 3,945,551 - hdfR b4480 0.48 -1.1 3,945,744 - hdfR b4480 0.71 -0.9 3,945,783 + hdfR b4480 0.75 -0.3 3,945,783 + hdfR b4480 0.75 -2.8 3,945,897 + hdfR b4480 0.89 -0.1 3,945,902 + hdfR b4480 0.89 -0.3 3,945,914 - -0.7 3,946,030 - -0.7 3,946,030 - +0.9 3,946,045 + -0.7 3,946,056 + -1.3 3,946,058 + -1.1 3,946,064 - -0.7 3,946,071 + +0.1 3,946,102 + -0.7 3,946,102 + -0.7 3,946,127 - -1.2 3,946,176 - yifE b3764 0.20 -1.3 3,946,179 - yifE b3764 0.21 +0.1 3,946,200 + yifE b3764 0.27 -0.3 3,946,281 - yifE b3764 0.51 -1.3 3,946,281 - yifE b3764 0.51 -0.3 3,946,295 - yifE b3764 0.55 -0.8 3,946,309 + yifE b3764 0.59 -1.3 3,946,345 + yifE b3764 0.70 -0.3 3,946,360 + yifE b3764 0.74 -0.7 3,946,360 + yifE b3764 0.74 -0.7 3,946,366 - yifE b3764 0.76 -1.3 3,946,366 - yifE b3764 0.76 -1.3 3,946,525 + -0.5 3,946,528 - +2.3 3,946,528 - +0.3 3,946,575 - -0.3 3,946,575 - +0.6 3,946,621 - -1.2 3,946,621 - -0.9 3,946,628 + yifB b3765 0.10 +0.2 3,946,632 + yifB b3765 0.11 -0.7 3,946,706 + yifB b3765 0.15 +0.1 3,946,761 + yifB b3765 0.19 +1.1 3,946,883 - yifB b3765 0.27 -1.0 3,946,903 - yifB b3765 0.28 +0.1 3,947,019 + yifB b3765 0.36 -0.3 3,947,019 + yifB b3765 0.36 -0.9 3,947,020 - yifB b3765 0.36 -1.8 3,947,047 + yifB b3765 0.38 +2.7 3,947,047 + yifB b3765 0.38 +0.1 3,947,065 - yifB b3765 0.39 -1.4 3,947,065 - yifB b3765 0.39 +0.1 3,947,070 - yifB b3765 0.39 -1.5 3,947,177 + yifB b3765 0.46 -0.2 3,947,178 - yifB b3765 0.46 +0.2 3,947,178 - yifB b3765 0.46 -1.5 3,947,180 + yifB b3765 0.47 -0.3 3,947,180 + yifB b3765 0.47 +0.0 3,947,195 - yifB b3765 0.48 -0.3 3,947,240 - yifB b3765 0.50 +0.5 3,947,251 - yifB b3765 0.51 -0.8 3,947,251 - yifB b3765 0.51 -0.2 3,947,269 - yifB b3765 0.52 -0.0 3,947,269 - yifB b3765 0.52 -0.0 3,947,339 - yifB b3765 0.57 -1.2 3,947,409 - yifB b3765 0.62 -0.5 3,947,423 - yifB b3765 0.63 -0.7 3,947,423 - yifB b3765 0.63 -0.4 3,947,423 - yifB b3765 0.63 -1.7 3,947,429 + yifB b3765 0.63 +0.5 3,947,429 + yifB b3765 0.63 -0.7 3,947,437 - yifB b3765 0.63 +0.4 3,947,449 - yifB b3765 0.64 -0.3 3,947,449 - yifB b3765 0.64 +0.5 3,947,479 - yifB b3765 0.66 +1.1 3,947,481 + yifB b3765 0.66 +0.3 3,947,506 - yifB b3765 0.68 -2.1 3,947,506 - yifB b3765 0.68 -0.1 3,947,523 + yifB b3765 0.69 +0.5 3,947,523 + yifB b3765 0.69 -1.9 3,947,532 - yifB b3765 0.70 -0.2 3,947,543 + yifB b3765 0.70 +0.5 3,947,543 + yifB b3765 0.70 -1.8 3,947,746 + yifB b3765 0.84 -0.5 3,947,833 - yifB b3765 0.89 -0.8 3,947,836 - yifB b3765 0.90 -1.8 3,947,841 + +0.8 3,947,903 + -0.0 3,947,903 + +0.3 3,947,984 - +0.7 3,948,303 + +0.9 3,948,305 + +0.3 3,948,364 + ilvL b3766 0.19 +0.7 3,948,375 + ilvL b3766 0.30 +1.1 3,948,383 - ilvL b3766 0.38 +1.1 3,948,410 + ilvL b3766 0.66 -0.4 3,948,410 + ilvL b3766 0.66 -0.9 3,948,410 + ilvL b3766 0.66 +1.3 3,948,531 - +0.5 3,948,579 + -0.5 3,948,580 + +0.1 3,948,580 + +0.7 3,948,604 + -0.7 3,948,604 + -0.1 3,948,620 + -0.3 3,948,620 + +1.4 3,948,624 + +0.4 3,948,633 - -0.3 3,948,633 - -0.9 3,948,633 - +0.3 3,948,682 + +0.4 3,948,721 + -0.9 3,948,726 + +0.1 3,948,748 + ilvG b4488 0.10 +1.3 3,948,748 + ilvG b4488 0.10 -0.9 3,948,781 + ilvG b4488 0.12 +0.3 3,948,815 + ilvG b4488 0.14 +0.3 3,948,815 + ilvG b4488 0.14 +0.9 3,948,851 + ilvG b4488 0.16 +0.9 3,948,851 + ilvG b4488 0.16 +0.2 3,948,851 + ilvG b4488 0.16 -0.7 3,948,859 - ilvG b4488 0.17 -0.2 3,948,933 + ilvG b4488 0.21 +0.3 3,948,939 + ilvG b4488 0.22 +0.0 3,948,944 + ilvG b4488 0.22 -0.3 3,948,947 - ilvG b4488 0.22 +0.0 3,948,947 - ilvG b4488 0.22 -0.2 3,948,966 - ilvG b4488 0.23 +0.1
Or see this region's nucleotide sequence