Experiment: Carbenicillin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt aegA and narQ are separated by 205 nucleotides narQ and acrD are separated by 163 nucleotides
b2468: aegA - fused predicted oxidoreductase: FeS binding subunit/NAD/FAD-binding subunit (NCBI), at 2,581,568 to 2,583,547
aegA
b2469: narQ - sensory histidine kinase in two-component regulatory system with NarP (NarL) (NCBI), at 2,583,753 to 2,585,453
narQ
b2470: acrD - aminoglycoside/multidrug efflux system (NCBI), at 2,585,617 to 2,588,730
acrD
Position (kb)
2583
2584
2585
2586 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 2582.757 kb on - strand, within aegA at 2582.809 kb on + strand, within aegA at 2582.809 kb on + strand, within aegA at 2582.817 kb on - strand, within aegA at 2582.824 kb on - strand, within aegA at 2582.824 kb on - strand, within aegA at 2582.827 kb on + strand, within aegA at 2582.883 kb on + strand, within aegA at 2582.883 kb on + strand, within aegA at 2582.970 kb on + strand, within aegA at 2583.092 kb on + strand, within aegA at 2583.099 kb on - strand, within aegA at 2583.118 kb on + strand, within aegA at 2583.374 kb on + strand at 2583.400 kb on + strand at 2583.451 kb on + strand at 2583.510 kb on + strand at 2583.609 kb on - strand at 2583.673 kb on - strand at 2583.698 kb on - strand at 2583.779 kb on - strand at 2583.788 kb on - strand at 2583.788 kb on - strand at 2583.986 kb on + strand, within narQ at 2583.996 kb on + strand, within narQ at 2584.021 kb on - strand, within narQ at 2584.107 kb on + strand, within narQ at 2584.107 kb on + strand, within narQ at 2584.112 kb on + strand, within narQ at 2584.137 kb on - strand, within narQ at 2584.152 kb on - strand, within narQ at 2584.167 kb on + strand, within narQ at 2584.172 kb on + strand, within narQ at 2584.172 kb on + strand, within narQ at 2584.314 kb on - strand, within narQ at 2584.409 kb on - strand, within narQ at 2584.422 kb on + strand, within narQ at 2584.422 kb on + strand, within narQ at 2584.523 kb on + strand, within narQ at 2584.541 kb on + strand, within narQ at 2584.577 kb on + strand, within narQ at 2584.695 kb on + strand, within narQ at 2584.695 kb on + strand, within narQ at 2584.931 kb on + strand, within narQ at 2584.950 kb on - strand, within narQ at 2584.976 kb on + strand, within narQ at 2584.987 kb on + strand, within narQ at 2585.017 kb on - strand, within narQ at 2585.121 kb on + strand, within narQ at 2585.128 kb on + strand, within narQ at 2585.270 kb on - strand, within narQ at 2585.357 kb on - strand at 2585.357 kb on - strand at 2585.362 kb on - strand at 2585.362 kb on - strand at 2585.407 kb on + strand at 2585.415 kb on - strand at 2585.456 kb on - strand at 2585.463 kb on - strand at 2585.483 kb on - strand at 2585.502 kb on - strand at 2585.553 kb on - strand at 2585.563 kb on - strand at 2585.563 kb on - strand at 2585.589 kb on + strand at 2585.589 kb on + strand at 2585.673 kb on - strand at 2585.673 kb on - strand at 2585.711 kb on + strand at 2585.760 kb on - strand at 2585.800 kb on + strand at 2585.800 kb on + strand at 2585.901 kb on + strand at 2586.050 kb on - strand, within acrD at 2586.050 kb on - strand, within acrD at 2586.063 kb on + strand, within acrD at 2586.109 kb on + strand, within acrD at 2586.109 kb on + strand, within acrD at 2586.283 kb on - strand, within acrD at 2586.283 kb on - strand, within acrD at 2586.384 kb on - strand, within acrD
Per-strain Table
Position Strand Gene LocusTag Fraction Carbenicillin 0.004 mg/ml remove 2,582,757 - aegA b2468 0.60 +1.0 2,582,809 + aegA b2468 0.63 +0.6 2,582,809 + aegA b2468 0.63 +2.7 2,582,817 - aegA b2468 0.63 +0.3 2,582,824 - aegA b2468 0.63 +1.0 2,582,824 - aegA b2468 0.63 +0.1 2,582,827 + aegA b2468 0.64 +1.2 2,582,883 + aegA b2468 0.66 +2.5 2,582,883 + aegA b2468 0.66 +0.1 2,582,970 + aegA b2468 0.71 +1.7 2,583,092 + aegA b2468 0.77 +1.0 2,583,099 - aegA b2468 0.77 -0.1 2,583,118 + aegA b2468 0.78 +0.8 2,583,374 + +1.1 2,583,400 + +1.6 2,583,451 + +1.7 2,583,510 + +1.3 2,583,609 - -0.7 2,583,673 - -0.3 2,583,698 - +0.9 2,583,779 - +0.2 2,583,788 - -0.3 2,583,788 - -1.3 2,583,986 + narQ b2469 0.14 +0.8 2,583,996 + narQ b2469 0.14 -0.1 2,584,021 - narQ b2469 0.16 -2.3 2,584,107 + narQ b2469 0.21 +4.0 2,584,107 + narQ b2469 0.21 +1.8 2,584,112 + narQ b2469 0.21 +1.5 2,584,137 - narQ b2469 0.23 -0.1 2,584,152 - narQ b2469 0.23 +0.6 2,584,167 + narQ b2469 0.24 +1.7 2,584,172 + narQ b2469 0.25 +0.7 2,584,172 + narQ b2469 0.25 +1.4 2,584,314 - narQ b2469 0.33 -0.2 2,584,409 - narQ b2469 0.39 +0.9 2,584,422 + narQ b2469 0.39 +1.9 2,584,422 + narQ b2469 0.39 +0.8 2,584,523 + narQ b2469 0.45 +1.2 2,584,541 + narQ b2469 0.46 +1.1 2,584,577 + narQ b2469 0.48 +0.9 2,584,695 + narQ b2469 0.55 +1.2 2,584,695 + narQ b2469 0.55 +0.9 2,584,931 + narQ b2469 0.69 +1.8 2,584,950 - narQ b2469 0.70 -1.5 2,584,976 + narQ b2469 0.72 +1.0 2,584,987 + narQ b2469 0.73 +1.2 2,585,017 - narQ b2469 0.74 +0.6 2,585,121 + narQ b2469 0.80 +1.7 2,585,128 + narQ b2469 0.81 +0.5 2,585,270 - narQ b2469 0.89 +0.5 2,585,357 - +0.3 2,585,357 - +0.7 2,585,362 - +0.8 2,585,362 - +1.9 2,585,407 + +1.7 2,585,415 - -1.7 2,585,456 - +0.8 2,585,463 - +1.8 2,585,483 - +1.7 2,585,502 - -0.2 2,585,553 - +0.4 2,585,563 - +0.3 2,585,563 - -1.4 2,585,589 + +1.4 2,585,589 + +1.2 2,585,673 - -2.1 2,585,673 - -1.1 2,585,711 + -2.3 2,585,760 - -1.3 2,585,800 + -1.8 2,585,800 + -1.4 2,585,901 + -0.7 2,586,050 - acrD b2470 0.14 -1.8 2,586,050 - acrD b2470 0.14 -3.2 2,586,063 + acrD b2470 0.14 -3.1 2,586,109 + acrD b2470 0.16 -0.7 2,586,109 + acrD b2470 0.16 -1.4 2,586,283 - acrD b2470 0.21 -1.1 2,586,283 - acrD b2470 0.21 -0.4 2,586,384 - acrD b2470 0.25 -3.2
Or see this region's nucleotide sequence