Experiment: Carbenicillin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pncB and pepN are separated by 265 nucleotides pepN and ssuB are separated by 42 nucleotides ssuB and ycbM overlap by 4 nucleotides
b0931: pncB - nicotinate phosphoribosyltransferase (NCBI), at 988,377 to 989,579
pncB
b0932: pepN - aminopeptidase N (NCBI), at 989,845 to 992,457
pepN
b0933: ssuB - alkanesulfonate transporter subunit (NCBI), at 992,500 to 993,267
ssuB
b0934: ycbM - putative transport system permease protein (VIMSS), at 993,264 to 994,055
ycbM
Position (kb) 989
990
991
992
993 Strain fitness (log2 ratio) -1
0
1
2
3 at 988.883 kb on + strand, within pncB at 989.105 kb on - strand, within pncB at 989.105 kb on - strand, within pncB at 989.167 kb on + strand, within pncB at 989.401 kb on + strand, within pncB at 989.401 kb on + strand, within pncB at 989.403 kb on - strand, within pncB at 989.445 kb on + strand, within pncB at 989.445 kb on + strand, within pncB at 989.493 kb on - strand at 989.493 kb on - strand at 989.541 kb on - strand at 989.541 kb on - strand at 989.718 kb on + strand at 989.726 kb on - strand at 989.726 kb on - strand at 989.771 kb on + strand at 989.771 kb on + strand at 989.771 kb on + strand at 989.798 kb on + strand at 989.809 kb on - strand at 989.810 kb on + strand at 989.819 kb on - strand at 989.819 kb on - strand at 989.828 kb on - strand at 990.041 kb on + strand at 990.059 kb on - strand at 990.142 kb on - strand, within pepN at 990.147 kb on + strand, within pepN at 990.147 kb on + strand, within pepN at 990.201 kb on + strand, within pepN at 990.209 kb on - strand, within pepN at 990.209 kb on - strand, within pepN at 990.357 kb on + strand, within pepN at 990.357 kb on + strand, within pepN at 990.433 kb on - strand, within pepN at 990.433 kb on - strand, within pepN at 990.499 kb on + strand, within pepN at 990.554 kb on + strand, within pepN at 990.554 kb on + strand, within pepN at 990.623 kb on - strand, within pepN at 990.623 kb on - strand, within pepN at 990.641 kb on - strand, within pepN at 990.641 kb on - strand, within pepN at 990.867 kb on + strand, within pepN at 990.968 kb on + strand, within pepN at 990.968 kb on + strand, within pepN at 990.982 kb on + strand, within pepN at 991.116 kb on - strand, within pepN at 991.116 kb on - strand, within pepN at 991.116 kb on - strand, within pepN at 991.168 kb on - strand, within pepN at 991.168 kb on - strand, within pepN at 991.236 kb on - strand, within pepN at 991.236 kb on - strand, within pepN at 991.236 kb on - strand, within pepN at 991.236 kb on - strand, within pepN at 991.250 kb on + strand, within pepN at 991.359 kb on + strand, within pepN at 991.359 kb on + strand, within pepN at 991.440 kb on + strand, within pepN at 991.786 kb on - strand, within pepN at 991.816 kb on + strand, within pepN at 991.847 kb on + strand, within pepN at 991.847 kb on + strand, within pepN at 991.892 kb on - strand, within pepN at 991.892 kb on - strand, within pepN at 991.968 kb on + strand, within pepN at 992.043 kb on - strand, within pepN at 992.045 kb on - strand, within pepN at 992.135 kb on - strand, within pepN at 992.149 kb on + strand, within pepN at 992.402 kb on - strand at 992.438 kb on + strand at 992.582 kb on - strand, within ssuB at 992.676 kb on + strand, within ssuB at 992.681 kb on + strand, within ssuB at 992.712 kb on + strand, within ssuB at 992.928 kb on - strand, within ssuB at 993.003 kb on + strand, within ssuB at 993.003 kb on + strand, within ssuB at 993.056 kb on - strand, within ssuB at 993.078 kb on - strand, within ssuB at 993.103 kb on + strand, within ssuB at 993.239 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Carbenicillin 0.004 mg/ml remove 988,883 + pncB b0931 0.42 +2.8 989,105 - pncB b0931 0.61 -0.5 989,105 - pncB b0931 0.61 -0.2 989,167 + pncB b0931 0.66 -1.6 989,401 + pncB b0931 0.85 +0.8 989,401 + pncB b0931 0.85 -0.6 989,403 - pncB b0931 0.85 -0.8 989,445 + pncB b0931 0.89 +0.3 989,445 + pncB b0931 0.89 -0.6 989,493 - -0.4 989,493 - -0.7 989,541 - +1.1 989,541 - +0.3 989,718 + +0.4 989,726 - +1.3 989,726 - +0.4 989,771 + -0.1 989,771 + -0.4 989,771 + +0.1 989,798 + +0.0 989,809 - +0.6 989,810 + -0.6 989,819 - -0.2 989,819 - +0.6 989,828 - +0.2 990,041 + -0.1 990,059 - -0.9 990,142 - pepN b0932 0.11 +1.0 990,147 + pepN b0932 0.12 -0.4 990,147 + pepN b0932 0.12 -0.2 990,201 + pepN b0932 0.14 +1.8 990,209 - pepN b0932 0.14 +2.8 990,209 - pepN b0932 0.14 -0.5 990,357 + pepN b0932 0.20 -0.1 990,357 + pepN b0932 0.20 +1.1 990,433 - pepN b0932 0.23 +0.8 990,433 - pepN b0932 0.23 +1.5 990,499 + pepN b0932 0.25 +0.2 990,554 + pepN b0932 0.27 -0.4 990,554 + pepN b0932 0.27 +1.0 990,623 - pepN b0932 0.30 +1.2 990,623 - pepN b0932 0.30 +0.8 990,641 - pepN b0932 0.30 +0.3 990,641 - pepN b0932 0.30 +0.4 990,867 + pepN b0932 0.39 -1.1 990,968 + pepN b0932 0.43 -0.2 990,968 + pepN b0932 0.43 +1.2 990,982 + pepN b0932 0.44 -1.2 991,116 - pepN b0932 0.49 +1.1 991,116 - pepN b0932 0.49 -0.2 991,116 - pepN b0932 0.49 +0.2 991,168 - pepN b0932 0.51 +0.6 991,168 - pepN b0932 0.51 +0.1 991,236 - pepN b0932 0.53 +0.8 991,236 - pepN b0932 0.53 +1.2 991,236 - pepN b0932 0.53 +0.2 991,236 - pepN b0932 0.53 +0.8 991,250 + pepN b0932 0.54 +0.8 991,359 + pepN b0932 0.58 -1.1 991,359 + pepN b0932 0.58 -0.2 991,440 + pepN b0932 0.61 +0.4 991,786 - pepN b0932 0.74 +0.8 991,816 + pepN b0932 0.75 -0.2 991,847 + pepN b0932 0.77 -1.2 991,847 + pepN b0932 0.77 -0.2 991,892 - pepN b0932 0.78 +0.2 991,892 - pepN b0932 0.78 -0.8 991,968 + pepN b0932 0.81 +0.2 992,043 - pepN b0932 0.84 +0.3 992,045 - pepN b0932 0.84 -0.8 992,135 - pepN b0932 0.88 +0.2 992,149 + pepN b0932 0.88 +0.2 992,402 - +0.3 992,438 + -0.2 992,582 - ssuB b0933 0.11 -0.1 992,676 + ssuB b0933 0.23 +0.1 992,681 + ssuB b0933 0.24 +1.4 992,712 + ssuB b0933 0.28 -0.0 992,928 - ssuB b0933 0.56 +1.3 993,003 + ssuB b0933 0.65 -0.2 993,003 + ssuB b0933 0.65 +0.6 993,056 - ssuB b0933 0.72 +0.3 993,078 - ssuB b0933 0.75 -0.6 993,103 + ssuB b0933 0.79 +0.2 993,239 - +0.1
Or see this region's nucleotide sequence