Experiment: 150_an_nonmag_3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS10840 and AMB_RS10845 are separated by 3 nucleotides AMB_RS10845 and AMB_RS10850 are separated by 23 nucleotides AMB_RS10850 and AMB_RS10855 are separated by 2 nucleotides
AMB_RS10840: AMB_RS10840 - 2-oxoacid:acceptor oxidoreductase subunit alpha, at 2,321,934 to 2,323,631
_RS10840
AMB_RS10845: AMB_RS10845 - 2-oxoglutarate synthase, at 2,323,635 to 2,324,474
_RS10845
AMB_RS10850: AMB_RS10850 - hypothetical protein, at 2,324,498 to 2,324,989
_RS10850
AMB_RS10855: AMB_RS10855 - RidA family protein, at 2,324,992 to 2,325,390
_RS10855
Position (kb)
2323
2324
2325 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5 at 2322.699 kb on + strand, within AMB_RS10840 at 2322.700 kb on - strand, within AMB_RS10840 at 2322.700 kb on - strand, within AMB_RS10840 at 2322.960 kb on + strand, within AMB_RS10840 at 2322.960 kb on + strand, within AMB_RS10840 at 2323.113 kb on + strand, within AMB_RS10840 at 2323.173 kb on + strand, within AMB_RS10840 at 2323.173 kb on + strand, within AMB_RS10840 at 2323.173 kb on + strand, within AMB_RS10840 at 2323.174 kb on - strand, within AMB_RS10840 at 2323.174 kb on - strand, within AMB_RS10840 at 2323.174 kb on - strand, within AMB_RS10840 at 2323.533 kb on + strand at 2323.533 kb on + strand at 2323.534 kb on - strand at 2323.534 kb on - strand at 2323.534 kb on - strand at 2323.629 kb on + strand at 2323.630 kb on - strand at 2323.630 kb on - strand at 2323.630 kb on - strand at 2323.630 kb on - strand at 2323.630 kb on - strand at 2323.630 kb on - strand at 2323.630 kb on - strand at 2323.652 kb on + strand at 2323.653 kb on - strand at 2323.653 kb on - strand at 2323.658 kb on + strand at 2323.663 kb on - strand at 2323.663 kb on - strand at 2323.663 kb on - strand at 2323.663 kb on - strand at 2323.722 kb on + strand, within AMB_RS10845 at 2323.723 kb on - strand, within AMB_RS10845 at 2323.828 kb on - strand, within AMB_RS10845 at 2323.839 kb on + strand, within AMB_RS10845 at 2323.839 kb on + strand, within AMB_RS10845 at 2324.001 kb on + strand, within AMB_RS10845 at 2324.001 kb on + strand, within AMB_RS10845 at 2324.001 kb on + strand, within AMB_RS10845 at 2324.001 kb on + strand, within AMB_RS10845 at 2324.001 kb on + strand, within AMB_RS10845 at 2324.002 kb on - strand, within AMB_RS10845 at 2324.002 kb on - strand, within AMB_RS10845 at 2324.002 kb on - strand, within AMB_RS10845 at 2324.025 kb on + strand, within AMB_RS10845 at 2324.025 kb on + strand, within AMB_RS10845 at 2324.412 kb on + strand at 2324.412 kb on + strand at 2324.748 kb on - strand, within AMB_RS10850 at 2324.748 kb on - strand, within AMB_RS10850 at 2324.748 kb on - strand, within AMB_RS10850 at 2324.951 kb on + strand at 2324.951 kb on + strand at 2324.951 kb on + strand at 2324.952 kb on - strand at 2324.997 kb on + strand at 2324.997 kb on + strand at 2324.998 kb on - strand at 2324.998 kb on - strand at 2324.998 kb on - strand at 2325.172 kb on - strand, within AMB_RS10855 at 2325.244 kb on + strand, within AMB_RS10855 at 2325.400 kb on + strand at 2325.400 kb on + strand at 2325.401 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 150_an_nonmag_3 remove 2,322,699 + AMB_RS10840 0.45 -1.2 2,322,700 - AMB_RS10840 0.45 -1.3 2,322,700 - AMB_RS10840 0.45 -0.2 2,322,960 + AMB_RS10840 0.60 -2.0 2,322,960 + AMB_RS10840 0.60 +5.5 2,323,113 + AMB_RS10840 0.69 -2.4 2,323,173 + AMB_RS10840 0.73 +0.4 2,323,173 + AMB_RS10840 0.73 +0.4 2,323,173 + AMB_RS10840 0.73 +0.0 2,323,174 - AMB_RS10840 0.73 -1.0 2,323,174 - AMB_RS10840 0.73 +0.9 2,323,174 - AMB_RS10840 0.73 -0.2 2,323,533 + +0.3 2,323,533 + -0.0 2,323,534 - -1.7 2,323,534 - -0.5 2,323,534 - -2.5 2,323,629 + -0.6 2,323,630 - +0.1 2,323,630 - -0.0 2,323,630 - +0.4 2,323,630 - -2.0 2,323,630 - -0.6 2,323,630 - -2.2 2,323,630 - -1.7 2,323,652 + -0.6 2,323,653 - -0.1 2,323,653 - -0.6 2,323,658 + -0.1 2,323,663 - -1.2 2,323,663 - -2.7 2,323,663 - -0.0 2,323,663 - +2.4 2,323,722 + AMB_RS10845 0.10 +0.6 2,323,723 - AMB_RS10845 0.10 -1.2 2,323,828 - AMB_RS10845 0.23 -0.8 2,323,839 + AMB_RS10845 0.24 -0.2 2,323,839 + AMB_RS10845 0.24 -0.2 2,324,001 + AMB_RS10845 0.44 -0.5 2,324,001 + AMB_RS10845 0.44 -1.7 2,324,001 + AMB_RS10845 0.44 +0.7 2,324,001 + AMB_RS10845 0.44 -0.2 2,324,001 + AMB_RS10845 0.44 +1.6 2,324,002 - AMB_RS10845 0.44 +0.2 2,324,002 - AMB_RS10845 0.44 -0.1 2,324,002 - AMB_RS10845 0.44 -1.6 2,324,025 + AMB_RS10845 0.46 -2.4 2,324,025 + AMB_RS10845 0.46 +0.7 2,324,412 + -1.9 2,324,412 + -0.3 2,324,748 - AMB_RS10850 0.51 -0.0 2,324,748 - AMB_RS10850 0.51 +0.2 2,324,748 - AMB_RS10850 0.51 -0.1 2,324,951 + +0.2 2,324,951 + +0.3 2,324,951 + -0.0 2,324,952 - +0.4 2,324,997 + -0.2 2,324,997 + -0.6 2,324,998 - -0.9 2,324,998 - +0.5 2,324,998 - -3.7 2,325,172 - AMB_RS10855 0.45 -1.8 2,325,244 + AMB_RS10855 0.63 -0.2 2,325,400 + +0.6 2,325,400 + -0.2 2,325,401 - -1.5
Or see this region's nucleotide sequence