Strain Fitness in Escherichia coli BW25113 around b4383

Experiment: Spectinomycin 0.0125 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntdeoA and deoB are separated by 51 nucleotidesdeoB and deoD are separated by 56 nucleotidesdeoD and yjjJ are separated by 166 nucleotides b4382: deoA - thymidine phosphorylase (NCBI), at 4,616,252 to 4,617,574 deoA b4383: deoB - phosphopentomutase (NCBI), at 4,617,626 to 4,618,849 deoB b4384: deoD - purine nucleoside phosphorylase (NCBI), at 4,618,906 to 4,619,625 deoD b4385: yjjJ - predicted DNA-binding transcriptional regulator (NCBI), at 4,619,792 to 4,621,123 yjjJ Position (kb) 4617 4618 4619Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 4616.663 kb on + strand, within deoAat 4616.727 kb on - strand, within deoAat 4616.731 kb on + strand, within deoAat 4616.749 kb on + strand, within deoAat 4616.760 kb on + strand, within deoAat 4616.791 kb on - strand, within deoAat 4616.823 kb on - strand, within deoAat 4616.940 kb on - strand, within deoAat 4616.958 kb on - strand, within deoAat 4616.958 kb on - strand, within deoAat 4616.965 kb on + strand, within deoAat 4616.984 kb on + strand, within deoAat 4616.987 kb on - strand, within deoAat 4616.987 kb on - strand, within deoAat 4616.997 kb on + strand, within deoAat 4617.084 kb on - strand, within deoAat 4617.088 kb on + strand, within deoAat 4617.154 kb on - strand, within deoAat 4617.158 kb on + strand, within deoAat 4617.193 kb on - strand, within deoAat 4617.258 kb on + strand, within deoAat 4617.278 kb on + strand, within deoAat 4617.278 kb on + strand, within deoAat 4617.285 kb on - strand, within deoAat 4617.386 kb on - strand, within deoAat 4617.390 kb on - strand, within deoAat 4617.390 kb on - strand, within deoAat 4617.396 kb on + strand, within deoAat 4617.396 kb on + strand, within deoAat 4617.613 kb on + strandat 4617.631 kb on - strandat 4617.647 kb on + strandat 4617.647 kb on + strandat 4617.647 kb on + strandat 4617.655 kb on - strandat 4617.680 kb on - strandat 4617.691 kb on + strandat 4617.726 kb on - strandat 4617.861 kb on + strand, within deoBat 4617.869 kb on - strand, within deoBat 4617.891 kb on - strand, within deoBat 4617.961 kb on - strand, within deoBat 4618.009 kb on + strand, within deoBat 4618.009 kb on + strand, within deoBat 4618.012 kb on + strand, within deoBat 4618.012 kb on + strand, within deoBat 4618.030 kb on + strand, within deoBat 4618.030 kb on + strand, within deoBat 4618.076 kb on + strand, within deoBat 4618.088 kb on + strand, within deoBat 4618.285 kb on + strand, within deoBat 4618.335 kb on + strand, within deoBat 4618.411 kb on + strandat 4618.411 kb on + strand, within deoBat 4618.411 kb on + strand, within deoBat 4618.411 kb on + strand, within deoBat 4618.512 kb on - strand, within deoBat 4618.512 kb on - strand, within deoBat 4618.528 kb on + strand, within deoBat 4618.553 kb on - strand, within deoBat 4618.587 kb on + strand, within deoBat 4618.587 kb on + strand, within deoBat 4618.641 kb on - strand, within deoBat 4618.646 kb on + strand, within deoBat 4618.646 kb on + strand, within deoBat 4618.681 kb on + strand, within deoBat 4618.681 kb on + strand, within deoBat 4618.687 kb on + strand, within deoBat 4618.689 kb on - strand, within deoBat 4618.714 kb on + strand, within deoBat 4618.718 kb on - strand, within deoBat 4618.718 kb on - strand, within deoBat 4618.732 kb on - strandat 4618.851 kb on - strandat 4618.864 kb on + strandat 4618.872 kb on - strandat 4618.977 kb on - strandat 4618.991 kb on - strand, within deoDat 4619.050 kb on - strand, within deoDat 4619.050 kb on - strand, within deoDat 4619.089 kb on + strand, within deoDat 4619.094 kb on + strand, within deoDat 4619.094 kb on + strand, within deoDat 4619.104 kb on + strand, within deoDat 4619.104 kb on + strand, within deoDat 4619.109 kb on + strand, within deoDat 4619.117 kb on - strand, within deoDat 4619.119 kb on + strand, within deoDat 4619.119 kb on + strand, within deoDat 4619.128 kb on + strand, within deoDat 4619.131 kb on + strand, within deoDat 4619.198 kb on + strand, within deoDat 4619.223 kb on + strand, within deoDat 4619.272 kb on + strand, within deoDat 4619.279 kb on + strand, within deoDat 4619.298 kb on + strand, within deoDat 4619.298 kb on + strand, within deoDat 4619.316 kb on - strand, within deoDat 4619.335 kb on + strand, within deoDat 4619.335 kb on + strand, within deoDat 4619.336 kb on - strand, within deoDat 4619.340 kb on + strand, within deoDat 4619.355 kb on + strand, within deoDat 4619.375 kb on - strand, within deoDat 4619.375 kb on - strand, within deoDat 4619.382 kb on + strand, within deoDat 4619.382 kb on + strand, within deoDat 4619.385 kb on - strand, within deoDat 4619.385 kb on - strand, within deoDat 4619.390 kb on - strand, within deoDat 4619.390 kb on - strand, within deoDat 4619.512 kb on + strand, within deoDat 4619.513 kb on + strand, within deoDat 4619.513 kb on + strand, within deoDat 4619.513 kb on + strand, within deoDat 4619.541 kb on + strand, within deoDat 4619.564 kb on - strandat 4619.564 kb on - strandat 4619.593 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Spectinomycin 0.0125 mg/ml
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4,616,663 + deoA b4382 0.31 +1.5
4,616,727 - deoA b4382 0.36 +0.7
4,616,731 + deoA b4382 0.36 +1.4
4,616,749 + deoA b4382 0.38 +0.8
4,616,760 + deoA b4382 0.38 +0.7
4,616,791 - deoA b4382 0.41 -0.0
4,616,823 - deoA b4382 0.43 +0.0
4,616,940 - deoA b4382 0.52 +0.6
4,616,958 - deoA b4382 0.53 -0.1
4,616,958 - deoA b4382 0.53 -0.1
4,616,965 + deoA b4382 0.54 +0.6
4,616,984 + deoA b4382 0.55 +0.4
4,616,987 - deoA b4382 0.56 +0.4
4,616,987 - deoA b4382 0.56 -0.5
4,616,997 + deoA b4382 0.56 +0.7
4,617,084 - deoA b4382 0.63 +0.5
4,617,088 + deoA b4382 0.63 +0.9
4,617,154 - deoA b4382 0.68 -0.3
4,617,158 + deoA b4382 0.68 +0.5
4,617,193 - deoA b4382 0.71 +0.7
4,617,258 + deoA b4382 0.76 +1.6
4,617,278 + deoA b4382 0.78 +1.2
4,617,278 + deoA b4382 0.78 +1.9
4,617,285 - deoA b4382 0.78 +0.6
4,617,386 - deoA b4382 0.86 +0.8
4,617,390 - deoA b4382 0.86 +0.2
4,617,390 - deoA b4382 0.86 +0.8
4,617,396 + deoA b4382 0.86 +1.8
4,617,396 + deoA b4382 0.86 +1.7
4,617,613 + +0.6
4,617,631 - -1.7
4,617,647 + -1.3
4,617,647 + -2.1
4,617,647 + -1.4
4,617,655 - -0.0
4,617,680 - -1.1
4,617,691 + +0.2
4,617,726 - -0.9
4,617,861 + deoB b4383 0.19 +0.2
4,617,869 - deoB b4383 0.20 -2.6
4,617,891 - deoB b4383 0.22 -0.3
4,617,961 - deoB b4383 0.27 -2.2
4,618,009 + deoB b4383 0.31 -1.2
4,618,009 + deoB b4383 0.31 -1.3
4,618,012 + deoB b4383 0.32 -3.4
4,618,012 + deoB b4383 0.32 -0.9
4,618,030 + deoB b4383 0.33 -1.1
4,618,030 + deoB b4383 0.33 -1.8
4,618,076 + deoB b4383 0.37 +0.2
4,618,088 + deoB b4383 0.38 -1.6
4,618,285 + deoB b4383 0.54 -2.3
4,618,335 + deoB b4383 0.58 -1.3
4,618,411 + +1.2
4,618,411 + deoB b4383 0.64 -2.7
4,618,411 + deoB b4383 0.64 -2.4
4,618,411 + deoB b4383 0.64 +1.2
4,618,512 - deoB b4383 0.72 -0.4
4,618,512 - deoB b4383 0.72 -0.0
4,618,528 + deoB b4383 0.74 -1.6
4,618,553 - deoB b4383 0.76 -1.8
4,618,587 + deoB b4383 0.79 +0.4
4,618,587 + deoB b4383 0.79 +0.5
4,618,641 - deoB b4383 0.83 -0.9
4,618,646 + deoB b4383 0.83 -0.3
4,618,646 + deoB b4383 0.83 -0.9
4,618,681 + deoB b4383 0.86 +0.2
4,618,681 + deoB b4383 0.86 -1.4
4,618,687 + deoB b4383 0.87 -1.8
4,618,689 - deoB b4383 0.87 +0.2
4,618,714 + deoB b4383 0.89 -0.2
4,618,718 - deoB b4383 0.89 -0.4
4,618,718 - deoB b4383 0.89 -2.3
4,618,732 - -1.1
4,618,851 - -1.0
4,618,864 + +1.5
4,618,872 - -1.1
4,618,977 - -0.7
4,618,991 - deoD b4384 0.12 -1.4
4,619,050 - deoD b4384 0.20 -1.4
4,619,050 - deoD b4384 0.20 -1.3
4,619,089 + deoD b4384 0.25 -2.6
4,619,094 + deoD b4384 0.26 -0.8
4,619,094 + deoD b4384 0.26 +0.2
4,619,104 + deoD b4384 0.28 +0.9
4,619,104 + deoD b4384 0.28 -1.1
4,619,109 + deoD b4384 0.28 -1.6
4,619,117 - deoD b4384 0.29 +0.2
4,619,119 + deoD b4384 0.30 -1.0
4,619,119 + deoD b4384 0.30 +1.2
4,619,128 + deoD b4384 0.31 -0.6
4,619,131 + deoD b4384 0.31 -0.2
4,619,198 + deoD b4384 0.41 -0.2
4,619,223 + deoD b4384 0.44 -0.8
4,619,272 + deoD b4384 0.51 +0.8
4,619,279 + deoD b4384 0.52 -2.1
4,619,298 + deoD b4384 0.54 +0.2
4,619,298 + deoD b4384 0.54 +0.6
4,619,316 - deoD b4384 0.57 -1.8
4,619,335 + deoD b4384 0.60 +0.6
4,619,335 + deoD b4384 0.60 -0.4
4,619,336 - deoD b4384 0.60 -0.1
4,619,340 + deoD b4384 0.60 -0.0
4,619,355 + deoD b4384 0.62 +0.2
4,619,375 - deoD b4384 0.65 -2.5
4,619,375 - deoD b4384 0.65 +0.2
4,619,382 + deoD b4384 0.66 -1.4
4,619,382 + deoD b4384 0.66 -1.1
4,619,385 - deoD b4384 0.67 -2.1
4,619,385 - deoD b4384 0.67 -0.7
4,619,390 - deoD b4384 0.67 -1.7
4,619,390 - deoD b4384 0.67 -0.4
4,619,512 + deoD b4384 0.84 +0.2
4,619,513 + deoD b4384 0.84 -2.0
4,619,513 + deoD b4384 0.84 +0.5
4,619,513 + deoD b4384 0.84 -0.9
4,619,541 + deoD b4384 0.88 -1.1
4,619,564 - -2.7
4,619,564 - -1.0
4,619,593 + -0.6

Or see this region's nucleotide sequence