Strain Fitness in Escherichia coli BW25113 around b4298

Experiment: Spectinomycin 0.0125 mg/ml

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	Spectinomycin 0.0125 mg/ml
4,521,142	+	yjhG	b4297	0.50	-0.1
4,521,144	-	yjhG	b4297	0.51	-0.7
4,521,144	-	yjhG	b4297	0.51	-0.1
4,521,173	+	yjhG	b4297	0.52	+1.0
4,521,173	+	yjhG	b4297	0.52	+0.3
4,521,202	+	yjhG	b4297	0.53	+0.2
4,521,280	+	yjhG	b4297	0.57	+0.9
4,521,299	-	yjhG	b4297	0.58	+0.0
4,521,299	-	yjhG	b4297	0.58	+0.2
4,521,317	+	yjhG	b4297	0.59	+0.2
4,521,331	-	yjhG	b4297	0.60	-0.0
4,521,354	-	yjhG	b4297	0.61	+0.8
4,521,357	+	yjhG	b4297	0.61	+0.5
4,521,388	+	yjhG	b4297	0.63	+1.2
4,521,478	-	yjhG	b4297	0.67	-2.3
4,521,478	-	yjhG	b4297	0.67	-0.1
4,521,499	-	yjhG	b4297	0.69	-0.6
4,521,509	+	yjhG	b4297	0.69	+0.5
4,521,509	+	yjhG	b4297	0.69	-0.4
4,521,604	+	yjhG	b4297	0.74	-0.3
4,521,746	-	yjhG	b4297	0.81	+1.5
4,521,751	-	yjhG	b4297	0.81	-0.7
4,521,774	-	yjhG	b4297	0.83	+0.9
4,521,774	-	yjhG	b4297	0.83	+0.7
4,521,776	+	yjhG	b4297	0.83	+1.2
4,521,776	+	yjhG	b4297	0.83	-1.4
4,521,778	+	yjhG	b4297	0.83	+0.5
4,521,783	+	yjhG	b4297	0.83	-1.3
4,521,791	-	yjhG	b4297	0.83	-0.6
4,521,791	-	yjhG	b4297	0.83	-0.0
4,521,796	-	yjhG	b4297	0.84	-0.4
4,521,796	-	yjhG	b4297	0.84	+0.6
4,521,855	+	yjhG	b4297	0.87	+1.2
4,521,855	+	yjhG	b4297	0.87	-1.1
4,521,861	-	yjhG	b4297	0.87	-0.1
4,521,861	-	yjhG	b4297	0.87	+0.8
4,521,879	+	yjhG	b4297	0.88	+1.2
4,521,879	+	yjhG	b4297	0.88	-0.7
4,521,910	+	yjhG	b4297	0.89	-0.4
4,521,918	-	yjhG	b4297	0.90	+2.8
4,521,918	-	yjhG	b4297	0.90	-0.4
4,521,920	-	yjhG	b4297	0.90	-0.3
4,521,982	-				+0.5
4,522,069	-				+0.3
4,522,069	-				+0.3
4,522,085	+				+0.2
4,522,085	+				+1.5
4,522,158	-				+0.2
4,522,234	+	yjhH	b4298	0.12	+0.8
4,522,234	+	yjhH	b4298	0.12	-0.8
4,522,353	-	yjhH	b4298	0.25	-0.4
4,522,432	+	yjhH	b4298	0.34	+0.2
4,522,432	+	yjhH	b4298	0.34	-0.1
4,522,610	-	yjhH	b4298	0.53	-0.3
4,522,610	-	yjhH	b4298	0.53	-0.2
4,522,629	+	yjhH	b4298	0.55	-1.5
4,522,652	+	yjhH	b4298	0.58	-0.4
4,522,702	+	yjhH	b4298	0.63	+0.8
4,522,750	+	yjhH	b4298	0.69	+1.2
4,522,760	+	yjhH	b4298	0.70	+2.0
4,522,760	+	yjhH	b4298	0.70	+0.2
4,522,814	-	yjhH	b4298	0.76	-1.3
4,522,833	+	yjhH	b4298	0.78	+0.7
4,522,843	+	yjhH	b4298	0.79	+0.2
4,522,849	+	yjhH	b4298	0.80	+2.2
4,522,849	+	yjhH	b4298	0.80	+0.4
4,522,855	+	yjhH	b4298	0.80	-2.4
4,522,855	+	yjhH	b4298	0.80	+1.2
4,522,856	-	yjhH	b4298	0.80	-0.1
4,522,860	+	yjhH	b4298	0.81	+2.9
4,522,860	+	yjhH	b4298	0.81	+0.0
4,522,860	+	yjhH	b4298	0.81	+2.6
4,522,865	+	yjhH	b4298	0.81	+0.1
4,522,865	+	yjhH	b4298	0.81	+0.9
4,522,867	+	yjhH	b4298	0.82	+0.6
4,522,870	+	yjhH	b4298	0.82	+0.5
4,522,870	+	yjhH	b4298	0.82	+1.2
4,522,870	+	yjhH	b4298	0.82	-0.3
4,522,878	-	yjhH	b4298	0.83	+0.3
4,522,886	+	yjhH	b4298	0.84	+0.6
4,522,886	+	yjhH	b4298	0.84	+1.8
4,522,886	+	yjhH	b4298	0.84	+0.5
4,522,886	+	yjhH	b4298	0.84	+1.0
4,522,894	+	yjhH	b4298	0.85	-1.4
4,522,894	+	yjhH	b4298	0.85	+1.2
4,522,903	+	yjhH	b4298	0.86	+0.6
4,522,903	+	yjhH	b4298	0.86	+0.3
4,522,904	+	yjhH	b4298	0.86	+1.5
4,522,904	-	yjhH	b4298	0.86	-1.1
4,522,911	-	yjhH	b4298	0.86	+0.5
4,522,923	-	yjhH	b4298	0.88	+0.2
4,522,998	+				-1.3
4,523,048	+				+0.1
4,523,056	-				-0.3
4,523,056	-				+2.2
4,523,071	+				-0.6
4,523,079	-				+0.4
4,523,082	+				-0.4
4,523,086	+				-0.4
4,523,087	+				+1.6
4,523,087	+				+0.6
4,523,126	-	yjhI	b4299	0.11	-1.4
4,523,126	-	yjhI	b4299	0.11	-1.3
4,523,178	-	yjhI	b4299	0.18	+0.8
4,523,178	-	yjhI	b4299	0.18	-0.8
4,523,183	-	yjhI	b4299	0.18	+0.5
4,523,184	+	yjhI	b4299	0.19	-0.5
4,523,202	-	yjhI	b4299	0.21	-0.3
4,523,225	-	yjhI	b4299	0.24	+0.2
4,523,225	-	yjhI	b4299	0.24	-0.4
4,523,227	-	yjhI	b4299	0.24	-0.7
4,523,227	-	yjhI	b4299	0.24	+0.2
4,523,227	-	yjhI	b4299	0.24	+2.5
4,523,227	-	yjhI	b4299	0.24	+0.1
4,523,227	-	yjhI	b4299	0.24	+0.8
4,523,227	-	yjhI	b4299	0.24	-1.2
4,523,227	-	yjhI	b4299	0.24	+0.5
4,523,227	-	yjhI	b4299	0.24	+0.3
4,523,227	-	yjhI	b4299	0.24	-0.3
4,523,227	-	yjhI	b4299	0.24	+0.9
4,523,239	-	yjhI	b4299	0.25	+0.9
4,523,239	-	yjhI	b4299	0.25	-0.3
4,523,240	-	yjhI	b4299	0.26	+1.2
4,523,259	-	yjhI	b4299	0.28	+0.8
4,523,262	-	yjhI	b4299	0.28	+0.2
4,523,262	-	yjhI	b4299	0.28	+0.3
4,523,262	-	yjhI	b4299	0.28	-0.7
4,523,262	-	yjhI	b4299	0.28	+0.0
4,523,262	-	yjhI	b4299	0.28	-1.3
4,523,262	-	yjhI	b4299	0.28	+0.6
4,523,262	-	yjhI	b4299	0.28	+0.8
4,523,262	-	yjhI	b4299	0.28	-0.3
4,523,262	-	yjhI	b4299	0.28	+0.1
4,523,262	-	yjhI	b4299	0.28	+0.8
4,523,262	-	yjhI	b4299	0.28	+1.6
4,523,262	-	yjhI	b4299	0.28	+0.9
4,523,262	-	yjhI	b4299	0.28	+1.2
4,523,262	-	yjhI	b4299	0.28	-0.4
4,523,265	+	yjhI	b4299	0.29	+0.9
4,523,265	+	yjhI	b4299	0.29	+0.2
4,523,266	+	yjhI	b4299	0.29	-0.9
4,523,266	+	yjhI	b4299	0.29	-1.0
4,523,273	-	yjhI	b4299	0.30	+0.1
4,523,273	-	yjhI	b4299	0.30	+0.6
4,523,326	-	yjhI	b4299	0.37	+1.0
4,523,328	-	yjhI	b4299	0.37	-1.1
4,523,339	-	yjhI	b4299	0.38	+0.5
4,523,391	-	yjhI	b4299	0.45	-1.1
4,523,392	-	yjhI	b4299	0.45	-0.2
4,523,475	-	yjhI	b4299	0.55	+0.4
4,523,475	-	yjhI	b4299	0.55	+1.6
4,523,540	+	yjhI	b4299	0.64	+0.5
4,523,771	-				-0.6
4,523,771	-				+0.1
4,523,779	-				-0.2
4,523,844	-				-0.2
4,523,844	-				-1.8
4,523,844	-				+0.1
4,523,911	-				-0.4
4,523,937	-				-0.4
4,523,988	-				-0.1
4,523,996	-				+0.5
4,524,000	+				-1.7
4,524,000	+				-0.1
4,524,003	+				-1.5
4,524,008	-				-0.5
4,524,008	-				-0.2
4,524,008	-				-0.0
4,524,008	-				+2.1
4,524,008	-				-1.8
4,524,009	+				+0.0
4,524,009	+				+0.8
4,524,009	+				-0.5
4,524,009	+				+0.5
4,524,009	-				-0.4
4,524,011	+				+0.2
4,524,011	+				-0.7
4,524,011	+				+0.7
4,524,011	+				-0.0
4,524,011	+				+0.7
4,524,011	-				-0.8
4,524,017	-				-0.2
4,524,017	-				-0.2
4,524,017	-				+1.5
4,524,017	-				+1.3
4,524,017	-				+0.9
4,524,017	-				+0.1
4,524,017	-				-0.6
4,524,018	-				-0.1
4,524,018	-				+0.3
4,524,019	-				+0.9
4,524,019	-				-0.9
4,524,019	-				+0.3
4,524,019	-				+0.1
4,524,027	-				+1.2
4,524,029	+				-3.0