Strain Fitness in Escherichia coli BW25113 around b4106

Experiment: Spectinomycin 0.0125 mg/ml

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	Spectinomycin 0.0125 mg/ml
4,321,421	-				-0.2
4,321,440	-				-2.3
4,321,440	-				-0.7
4,321,512	-	phnD	b4105	0.15	-0.0
4,321,572	+	phnD	b4105	0.21	+0.6
4,321,637	-	phnD	b4105	0.27	-0.1
4,321,637	-	phnD	b4105	0.27	+0.7
4,321,713	-	phnD	b4105	0.35	-0.9
4,321,713	-	phnD	b4105	0.35	-0.8
4,321,716	+	phnD	b4105	0.35	+0.5
4,321,834	+	phnD	b4105	0.47	+1.2
4,321,834	+	phnD	b4105	0.47	+0.2
4,321,915	-	phnD	b4105	0.55	-0.5
4,322,004	+	phnD	b4105	0.63	+0.1
4,322,088	-	phnD	b4105	0.72	+0.0
4,322,155	+	phnD	b4105	0.78	+0.7
4,322,155	+	phnD	b4105	0.78	-0.2
4,322,249	-	phnD	b4105	0.88	+0.5
4,322,310	+				+0.8
4,322,328	+				+0.6
4,322,335	-				-0.3
4,322,415	+				+0.7
4,322,415	+				+0.5
4,322,429	+				-0.0
4,322,463	+				+0.3
4,322,471	+				-0.3
4,322,471	+				-0.1
4,322,525	+	phnC	b4106	0.16	+1.2
4,322,538	-	phnC	b4106	0.17	-0.1
4,322,563	+	phnC	b4106	0.21	+1.0
4,322,563	+	phnC	b4106	0.21	+0.6
4,322,841	-	phnC	b4106	0.56	-1.5
4,322,856	-	phnC	b4106	0.58	+0.3
4,322,915	+	phnC	b4106	0.65	-0.8
4,322,915	+	phnC	b4106	0.65	-0.1
4,322,963	-	phnC	b4106	0.71	+0.4
4,322,988	-	phnC	b4106	0.75	-0.4
4,323,255	+				-0.3
4,323,352	+				-0.8
4,323,369	+	phnB	b4107	0.11	+1.3
4,323,369	+	phnB	b4107	0.11	-0.0
4,323,387	+	phnB	b4107	0.15	-0.3
4,323,500	+	phnB	b4107	0.40	+0.2
4,323,544	-	phnB	b4107	0.50	+0.1
4,323,556	-	phnB	b4107	0.53	+1.3
4,323,556	-	phnB	b4107	0.53	-0.3
4,323,568	+	phnB	b4107	0.56	+0.7
4,323,613	+	phnB	b4107	0.66	+0.5
4,323,686	-	phnB	b4107	0.82	-2.1
4,323,686	-	phnB	b4107	0.82	-0.0
4,323,686	-	phnB	b4107	0.82	+0.6
4,323,769	-				+1.3
4,323,769	-				+0.3
4,323,776	+				+0.6
4,323,826	-				+0.9
4,323,860	-				+0.6
4,324,091	+				-1.3
4,324,091	+				+0.6
4,324,101	+				-1.1