Strain Fitness in Escherichia coli BW25113 around b4101

Experiment: Spectinomycin 0.0125 mg/ml

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	Spectinomycin 0.0125 mg/ml
4,318,321	-	phnI	b4099	0.66	+0.9
4,318,326	-	phnI	b4099	0.66	-0.5
4,318,392	+	phnI	b4099	0.72	+0.3
4,318,400	+	phnI	b4099	0.73	+0.5
4,318,489	-	phnI	b4099	0.81	+0.1
4,318,549	+	phnI	b4099	0.87	-0.2
4,318,635	-				+0.1
4,318,635	-				-1.1
4,318,687	-				-1.1
4,318,702	-				-0.5
4,318,748	-	phnH	b4100	0.11	-0.2
4,318,799	-	phnH	b4100	0.19	+0.1
4,318,814	+	phnH	b4100	0.22	-0.2
4,318,855	+	phnH	b4100	0.29	+0.1
4,318,855	+	phnH	b4100	0.29	+0.0
4,319,165	+	phnH	b4100	0.82	+0.3
4,319,223	+				+0.3
4,319,223	+				-0.3
4,319,223	+				+0.0
4,319,228	+				+0.2
4,319,258	+				+0.7
4,319,258	+				+0.6
4,319,259	+				-1.2
4,319,274	-				-1.3
4,319,279	-				-0.7
4,319,279	-				-0.7
4,319,354	-	phnG	b4101	0.19	-2.0
4,319,366	-	phnG	b4101	0.22	-2.6
4,319,379	-	phnG	b4101	0.25	-0.8
4,319,434	-	phnG	b4101	0.37	+1.3
4,319,446	-	phnG	b4101	0.40	-1.1
4,319,573	+	phnG	b4101	0.68	-1.3
4,319,731	-				-1.4
4,319,731	-				-0.0
4,319,803	+	phnF	b4102	0.11	+1.6
4,319,803	+	phnF	b4102	0.11	+2.3
4,319,803	+	phnF	b4102	0.11	-0.3
4,319,811	-	phnF	b4102	0.13	-1.5
4,319,811	-	phnF	b4102	0.13	-0.3
4,319,985	-	phnF	b4102	0.37	+0.2
4,319,999	+	phnF	b4102	0.38	-1.0
4,320,073	-	phnF	b4102	0.49	-0.8
4,320,121	-	phnF	b4102	0.55	-0.4
4,320,253	-	phnF	b4102	0.73	-0.5
4,320,253	-	phnF	b4102	0.73	-1.0
4,320,292	+	phnF	b4102	0.79	+0.3
4,320,326	+	phnF	b4102	0.83	+1.3
4,320,348	-	phnF	b4102	0.87	-0.9
4,320,405	-				-0.8
4,320,412	+				+0.3
4,320,412	+				+0.9
4,320,652	-	phnE	b4104	0.50	+0.1
4,320,670	-	phnE	b4104	0.55	-0.7
4,320,678	-	phnE	b4104	0.57	-2.8
4,320,691	+	phnE	b4104	0.60	+0.6