Strain Fitness in Escherichia coli BW25113 around b3827

Experiment: Spectinomycin 0.0125 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntpldB and yigL are separated by 7 nucleotidesyigL and yigM are separated by 75 nucleotidesyigM and metR overlap by 113 nucleotides b3825: pldB - lysophospholipase L(2) (NCBI), at 4,007,193 to 4,008,215 pldB b3826: yigL - predicted hydrolase (NCBI), at 4,008,223 to 4,009,023 yigL b3827: yigM - predicted inner membrane protein (NCBI), at 4,009,099 to 4,009,998 yigM b3828: metR - DNA-binding transcriptional activator, homocysteine-binding (NCBI), at 4,009,886 to 4,010,839 metR Position (kb) 4009 4010Strain fitness (log2 ratio) -2 -1 0 1 2 3at 4008.225 kb on + strandat 4008.230 kb on + strandat 4008.230 kb on + strandat 4008.302 kb on + strandat 4008.302 kb on + strandat 4008.310 kb on - strand, within yigLat 4008.350 kb on + strand, within yigLat 4008.350 kb on + strand, within yigLat 4008.393 kb on - strand, within yigLat 4008.416 kb on + strand, within yigLat 4008.430 kb on + strand, within yigLat 4008.489 kb on - strand, within yigLat 4008.495 kb on + strand, within yigLat 4008.496 kb on - strand, within yigLat 4008.505 kb on + strand, within yigLat 4008.526 kb on - strand, within yigLat 4008.526 kb on - strand, within yigLat 4008.529 kb on + strand, within yigLat 4008.572 kb on + strand, within yigLat 4008.572 kb on + strand, within yigLat 4008.621 kb on + strand, within yigLat 4008.629 kb on - strand, within yigLat 4008.629 kb on - strand, within yigLat 4008.629 kb on - strand, within yigLat 4008.632 kb on + strand, within yigLat 4008.677 kb on + strand, within yigLat 4008.677 kb on + strand, within yigLat 4008.680 kb on + strand, within yigLat 4008.688 kb on - strand, within yigLat 4008.708 kb on + strand, within yigLat 4008.754 kb on + strand, within yigLat 4008.754 kb on + strand, within yigLat 4008.754 kb on + strand, within yigLat 4008.754 kb on + strand, within yigLat 4008.762 kb on - strand, within yigLat 4008.762 kb on - strand, within yigLat 4008.903 kb on - strand, within yigLat 4008.910 kb on + strand, within yigLat 4008.918 kb on - strand, within yigLat 4008.920 kb on + strand, within yigLat 4008.920 kb on + strand, within yigLat 4008.920 kb on + strand, within yigLat 4008.968 kb on + strandat 4009.067 kb on - strandat 4009.082 kb on - strandat 4009.134 kb on - strandat 4009.176 kb on - strandat 4009.176 kb on - strandat 4009.205 kb on - strand, within yigMat 4009.275 kb on + strand, within yigMat 4009.275 kb on + strand, within yigMat 4009.275 kb on + strand, within yigMat 4009.275 kb on + strand, within yigMat 4009.275 kb on + strand, within yigMat 4009.275 kb on + strand, within yigMat 4009.283 kb on - strand, within yigMat 4009.283 kb on - strand, within yigMat 4009.311 kb on - strand, within yigMat 4009.334 kb on + strand, within yigMat 4009.334 kb on + strand, within yigMat 4009.338 kb on - strand, within yigMat 4009.348 kb on - strand, within yigMat 4009.348 kb on - strand, within yigMat 4009.406 kb on - strand, within yigMat 4009.428 kb on + strand, within yigMat 4009.434 kb on + strand, within yigMat 4009.434 kb on + strand, within yigMat 4009.451 kb on - strand, within yigMat 4009.495 kb on - strand, within yigMat 4009.495 kb on - strand, within yigMat 4009.506 kb on + strand, within yigMat 4009.524 kb on - strand, within yigMat 4009.600 kb on + strand, within yigMat 4009.772 kb on + strand, within yigMat 4009.839 kb on - strand, within yigMat 4009.839 kb on - strand, within yigMat 4009.845 kb on - strand, within yigMat 4009.845 kb on - strand, within yigMat 4009.874 kb on + strand, within yigMat 4009.904 kb on + strandat 4009.917 kb on - strandat 4009.921 kb on - strandat 4009.921 kb on - strandat 4009.947 kb on - strandat 4010.026 kb on - strand, within metRat 4010.026 kb on - strand, within metRat 4010.038 kb on - strand, within metRat 4010.048 kb on - strand, within metRat 4010.094 kb on - strand, within metRat 4010.094 kb on - strand, within metRat 4010.094 kb on - strand, within metRat 4010.100 kb on - strand, within metRat 4010.113 kb on + strand, within metRat 4010.152 kb on - strand, within metRat 4010.245 kb on + strand, within metRat 4010.247 kb on + strand, within metRat 4010.267 kb on + strand, within metRat 4010.267 kb on + strand, within metRat 4010.337 kb on - strand, within metRat 4010.353 kb on + strand, within metRat 4010.386 kb on - strand, within metRat 4010.386 kb on - strand, within metRat 4010.386 kb on - strand, within metRat 4010.461 kb on - strand, within metRat 4010.461 kb on - strand, within metRat 4010.541 kb on + strand, within metRat 4010.541 kb on + strand, within metRat 4010.546 kb on - strand, within metRat 4010.549 kb on - strand, within metRat 4010.560 kb on - strand, within metRat 4010.560 kb on - strand, within metRat 4010.572 kb on - strand, within metRat 4010.572 kb on - strand, within metRat 4010.677 kb on - strand, within metRat 4010.729 kb on + strand, within metRat 4010.969 kb on + strandat 4010.977 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Spectinomycin 0.0125 mg/ml
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4,008,225 + -1.1
4,008,230 + -1.0
4,008,230 + -0.4
4,008,302 + -1.6
4,008,302 + -0.7
4,008,310 - yigL b3826 0.11 -0.4
4,008,350 + yigL b3826 0.16 +1.2
4,008,350 + yigL b3826 0.16 +1.1
4,008,393 - yigL b3826 0.21 +0.2
4,008,416 + yigL b3826 0.24 +0.4
4,008,430 + yigL b3826 0.26 +1.2
4,008,489 - yigL b3826 0.33 -0.6
4,008,495 + yigL b3826 0.34 -1.1
4,008,496 - yigL b3826 0.34 -1.0
4,008,505 + yigL b3826 0.35 -0.7
4,008,526 - yigL b3826 0.38 +1.4
4,008,526 - yigL b3826 0.38 -0.4
4,008,529 + yigL b3826 0.38 -0.4
4,008,572 + yigL b3826 0.44 -1.6
4,008,572 + yigL b3826 0.44 -1.2
4,008,621 + yigL b3826 0.50 -0.4
4,008,629 - yigL b3826 0.51 +0.9
4,008,629 - yigL b3826 0.51 +0.0
4,008,629 - yigL b3826 0.51 +1.3
4,008,632 + yigL b3826 0.51 +2.4
4,008,677 + yigL b3826 0.57 +0.6
4,008,677 + yigL b3826 0.57 -0.1
4,008,680 + yigL b3826 0.57 +0.6
4,008,688 - yigL b3826 0.58 +0.5
4,008,708 + yigL b3826 0.61 -0.8
4,008,754 + yigL b3826 0.66 -1.0
4,008,754 + yigL b3826 0.66 -2.5
4,008,754 + yigL b3826 0.66 -0.6
4,008,754 + yigL b3826 0.66 +0.7
4,008,762 - yigL b3826 0.67 +0.0
4,008,762 - yigL b3826 0.67 -1.6
4,008,903 - yigL b3826 0.85 -0.1
4,008,910 + yigL b3826 0.86 +0.9
4,008,918 - yigL b3826 0.87 -0.8
4,008,920 + yigL b3826 0.87 +1.0
4,008,920 + yigL b3826 0.87 +0.9
4,008,920 + yigL b3826 0.87 -0.1
4,008,968 + +0.0
4,009,067 - +0.9
4,009,082 - -0.1
4,009,134 - -0.9
4,009,176 - -0.2
4,009,176 - -0.1
4,009,205 - yigM b3827 0.12 +0.2
4,009,275 + yigM b3827 0.20 -0.0
4,009,275 + yigM b3827 0.20 -0.1
4,009,275 + yigM b3827 0.20 -0.6
4,009,275 + yigM b3827 0.20 -0.2
4,009,275 + yigM b3827 0.20 -0.5
4,009,275 + yigM b3827 0.20 -0.9
4,009,283 - yigM b3827 0.20 +0.3
4,009,283 - yigM b3827 0.20 +0.6
4,009,311 - yigM b3827 0.24 -1.9
4,009,334 + yigM b3827 0.26 +2.2
4,009,334 + yigM b3827 0.26 +1.4
4,009,338 - yigM b3827 0.27 +1.0
4,009,348 - yigM b3827 0.28 +2.0
4,009,348 - yigM b3827 0.28 +0.4
4,009,406 - yigM b3827 0.34 -0.3
4,009,428 + yigM b3827 0.37 -1.1
4,009,434 + yigM b3827 0.37 +0.6
4,009,434 + yigM b3827 0.37 -0.6
4,009,451 - yigM b3827 0.39 +0.9
4,009,495 - yigM b3827 0.44 -0.1
4,009,495 - yigM b3827 0.44 -0.4
4,009,506 + yigM b3827 0.45 +0.6
4,009,524 - yigM b3827 0.47 +2.1
4,009,600 + yigM b3827 0.56 -0.1
4,009,772 + yigM b3827 0.75 -0.8
4,009,839 - yigM b3827 0.82 -0.4
4,009,839 - yigM b3827 0.82 +2.9
4,009,845 - yigM b3827 0.83 +0.4
4,009,845 - yigM b3827 0.83 -1.4
4,009,874 + yigM b3827 0.86 +0.4
4,009,904 + +0.3
4,009,917 - -0.7
4,009,921 - +1.0
4,009,921 - +0.6
4,009,947 - +0.0
4,010,026 - metR b3828 0.15 +0.0
4,010,026 - metR b3828 0.15 -0.4
4,010,038 - metR b3828 0.16 +0.0
4,010,048 - metR b3828 0.17 -0.9
4,010,094 - metR b3828 0.22 +0.7
4,010,094 - metR b3828 0.22 -0.8
4,010,094 - metR b3828 0.22 +0.2
4,010,100 - metR b3828 0.22 -0.2
4,010,113 + metR b3828 0.24 -1.3
4,010,152 - metR b3828 0.28 +0.5
4,010,245 + metR b3828 0.38 +0.1
4,010,247 + metR b3828 0.38 +2.0
4,010,267 + metR b3828 0.40 +1.0
4,010,267 + metR b3828 0.40 +0.7
4,010,337 - metR b3828 0.47 +2.6
4,010,353 + metR b3828 0.49 +1.8
4,010,386 - metR b3828 0.52 -0.1
4,010,386 - metR b3828 0.52 +0.8
4,010,386 - metR b3828 0.52 -0.0
4,010,461 - metR b3828 0.60 +0.3
4,010,461 - metR b3828 0.60 +1.6
4,010,541 + metR b3828 0.69 -0.4
4,010,541 + metR b3828 0.69 -1.5
4,010,546 - metR b3828 0.69 -0.0
4,010,549 - metR b3828 0.69 +1.2
4,010,560 - metR b3828 0.71 +0.4
4,010,560 - metR b3828 0.71 +1.0
4,010,572 - metR b3828 0.72 -0.7
4,010,572 - metR b3828 0.72 +0.6
4,010,677 - metR b3828 0.83 +1.6
4,010,729 + metR b3828 0.88 -0.8
4,010,969 + +0.1
4,010,977 - +0.2

Or see this region's nucleotide sequence