Experiment: Spectinomycin 0.0125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt dinD and yicG are separated by 289 nucleotides yicG and yicF overlap by 4 nucleotides yicF and gmk are separated by 257 nucleotides gmk and rpoZ are separated by 54 nucleotides
b3645: dinD - DNA-damage-inducible protein (VIMSS), at 3,815,783 to 3,816,607
dinD
b3646: yicG - orf, hypothetical protein (VIMSS), at 3,816,897 to 3,817,514
yicG
b3647: yicF - putative enzyme (VIMSS), at 3,817,511 to 3,819,193
yicF
b3648: gmk - guanylate kinase (NCBI), at 3,819,451 to 3,820,074
gmk
b3649: rpoZ - DNA-directed RNA polymerase subunit omega (NCBI), at 3,820,129 to 3,820,404
rpoZ
Position (kb)
3817
3818
3819
3820 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3816.531 kb on + strand at 3816.561 kb on - strand at 3816.629 kb on + strand at 3816.632 kb on + strand at 3816.666 kb on + strand at 3816.670 kb on + strand at 3816.866 kb on - strand at 3816.908 kb on - strand at 3816.908 kb on - strand at 3816.908 kb on - strand at 3816.908 kb on - strand at 3816.934 kb on - strand at 3816.938 kb on + strand at 3816.938 kb on + strand at 3816.946 kb on - strand at 3816.947 kb on - strand at 3816.947 kb on - strand at 3816.961 kb on + strand, within yicG at 3816.966 kb on + strand, within yicG at 3816.966 kb on + strand, within yicG at 3816.986 kb on - strand, within yicG at 3816.986 kb on - strand, within yicG at 3817.027 kb on + strand, within yicG at 3817.027 kb on + strand, within yicG at 3817.051 kb on + strand, within yicG at 3817.052 kb on - strand, within yicG at 3817.057 kb on - strand, within yicG at 3817.087 kb on + strand, within yicG at 3817.087 kb on + strand, within yicG at 3817.146 kb on + strand, within yicG at 3817.149 kb on - strand, within yicG at 3817.149 kb on - strand, within yicG at 3817.207 kb on + strand, within yicG at 3817.215 kb on - strand, within yicG at 3817.231 kb on - strand, within yicG at 3817.285 kb on - strand, within yicG at 3817.285 kb on - strand, within yicG at 3817.328 kb on + strand, within yicG at 3817.328 kb on + strand, within yicG at 3817.335 kb on + strand, within yicG at 3817.345 kb on - strand, within yicG at 3817.371 kb on - strand, within yicG at 3817.376 kb on - strand, within yicG at 3817.376 kb on - strand, within yicG at 3817.378 kb on - strand, within yicG at 3817.391 kb on + strand, within yicG at 3817.401 kb on + strand, within yicG at 3817.401 kb on + strand, within yicG at 3817.433 kb on - strand, within yicG at 3817.485 kb on + strand at 3817.506 kb on - strand at 3817.607 kb on + strand at 3817.615 kb on - strand at 3817.615 kb on - strand at 3817.640 kb on - strand at 3817.665 kb on + strand at 3817.670 kb on + strand at 3817.673 kb on - strand at 3817.673 kb on - strand at 3817.708 kb on + strand, within yicF at 3817.716 kb on - strand, within yicF at 3817.795 kb on + strand, within yicF at 3817.795 kb on + strand, within yicF at 3817.828 kb on - strand, within yicF at 3817.828 kb on - strand, within yicF at 3817.877 kb on - strand, within yicF at 3817.877 kb on - strand, within yicF at 3817.897 kb on + strand, within yicF at 3817.900 kb on - strand, within yicF at 3817.918 kb on - strand, within yicF at 3817.918 kb on - strand, within yicF at 3817.939 kb on + strand, within yicF at 3818.035 kb on + strand, within yicF at 3818.043 kb on - strand, within yicF at 3818.043 kb on - strand, within yicF at 3818.225 kb on - strand, within yicF at 3818.255 kb on - strand, within yicF at 3818.255 kb on - strand, within yicF at 3818.289 kb on + strand, within yicF at 3818.304 kb on + strand, within yicF at 3818.375 kb on - strand, within yicF at 3818.375 kb on - strand, within yicF at 3818.388 kb on + strand, within yicF at 3818.390 kb on + strand, within yicF at 3818.400 kb on + strand, within yicF at 3818.400 kb on + strand, within yicF at 3818.439 kb on + strand, within yicF at 3818.496 kb on + strand, within yicF at 3818.564 kb on + strand, within yicF at 3818.627 kb on + strand, within yicF at 3818.672 kb on + strand, within yicF at 3818.672 kb on + strand, within yicF at 3818.675 kb on + strand, within yicF at 3818.680 kb on - strand, within yicF at 3818.735 kb on + strand, within yicF at 3818.743 kb on - strand, within yicF at 3818.781 kb on + strand, within yicF at 3818.794 kb on + strand, within yicF at 3818.794 kb on + strand, within yicF at 3818.806 kb on + strand, within yicF at 3818.832 kb on + strand, within yicF at 3818.840 kb on - strand, within yicF at 3818.886 kb on + strand, within yicF at 3818.899 kb on + strand, within yicF at 3818.904 kb on - strand, within yicF at 3818.920 kb on + strand, within yicF at 3818.938 kb on - strand, within yicF at 3818.994 kb on - strand, within yicF at 3818.994 kb on - strand, within yicF at 3819.005 kb on - strand, within yicF at 3819.005 kb on - strand, within yicF at 3819.041 kb on - strand at 3819.053 kb on - strand at 3819.065 kb on - strand at 3819.111 kb on - strand at 3819.121 kb on + strand at 3819.121 kb on + strand at 3819.130 kb on - strand at 3819.173 kb on - strand at 3819.191 kb on + strand at 3819.197 kb on - strand at 3819.197 kb on - strand at 3819.212 kb on - strand at 3819.234 kb on + strand at 3819.252 kb on - strand at 3819.252 kb on - strand at 3819.303 kb on - strand at 3819.394 kb on - strand at 3819.394 kb on - strand at 3820.087 kb on - strand at 3820.111 kb on - strand at 3820.144 kb on + strand at 3820.144 kb on + strand at 3820.151 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.0125 mg/ml remove 3,816,531 + +0.5 3,816,561 - -1.0 3,816,629 + +0.3 3,816,632 + +0.6 3,816,666 + +1.3 3,816,670 + -0.0 3,816,866 - +0.6 3,816,908 - +0.0 3,816,908 - +1.0 3,816,908 - +0.6 3,816,908 - +1.2 3,816,934 - +0.9 3,816,938 + +1.8 3,816,938 + -0.7 3,816,946 - +0.2 3,816,947 - +0.7 3,816,947 - +0.7 3,816,961 + yicG b3646 0.10 +0.5 3,816,966 + yicG b3646 0.11 +1.3 3,816,966 + yicG b3646 0.11 +1.6 3,816,986 - yicG b3646 0.14 +1.5 3,816,986 - yicG b3646 0.14 -0.7 3,817,027 + yicG b3646 0.21 +1.3 3,817,027 + yicG b3646 0.21 +0.8 3,817,051 + yicG b3646 0.25 +2.5 3,817,052 - yicG b3646 0.25 +0.4 3,817,057 - yicG b3646 0.26 +0.8 3,817,087 + yicG b3646 0.31 +1.1 3,817,087 + yicG b3646 0.31 +0.1 3,817,146 + yicG b3646 0.40 +1.2 3,817,149 - yicG b3646 0.41 +1.1 3,817,149 - yicG b3646 0.41 +1.5 3,817,207 + yicG b3646 0.50 -0.1 3,817,215 - yicG b3646 0.51 +0.7 3,817,231 - yicG b3646 0.54 -1.5 3,817,285 - yicG b3646 0.63 +1.8 3,817,285 - yicG b3646 0.63 +0.6 3,817,328 + yicG b3646 0.70 +1.3 3,817,328 + yicG b3646 0.70 +1.8 3,817,335 + yicG b3646 0.71 +0.4 3,817,345 - yicG b3646 0.72 +3.3 3,817,371 - yicG b3646 0.77 +1.0 3,817,376 - yicG b3646 0.78 +0.7 3,817,376 - yicG b3646 0.78 +1.3 3,817,378 - yicG b3646 0.78 +1.2 3,817,391 + yicG b3646 0.80 +1.7 3,817,401 + yicG b3646 0.82 +2.1 3,817,401 + yicG b3646 0.82 +0.5 3,817,433 - yicG b3646 0.87 +0.4 3,817,485 + +0.4 3,817,506 - +0.5 3,817,607 + +0.2 3,817,615 - +2.5 3,817,615 - -0.3 3,817,640 - +1.2 3,817,665 + +0.1 3,817,670 + -0.1 3,817,673 - +0.4 3,817,673 - +0.6 3,817,708 + yicF b3647 0.12 +0.5 3,817,716 - yicF b3647 0.12 +2.1 3,817,795 + yicF b3647 0.17 -1.1 3,817,795 + yicF b3647 0.17 -1.0 3,817,828 - yicF b3647 0.19 +1.2 3,817,828 - yicF b3647 0.19 +1.9 3,817,877 - yicF b3647 0.22 +0.0 3,817,877 - yicF b3647 0.22 +1.0 3,817,897 + yicF b3647 0.23 +0.9 3,817,900 - yicF b3647 0.23 +0.7 3,817,918 - yicF b3647 0.24 +1.7 3,817,918 - yicF b3647 0.24 +1.9 3,817,939 + yicF b3647 0.25 -0.4 3,818,035 + yicF b3647 0.31 -0.5 3,818,043 - yicF b3647 0.32 +0.5 3,818,043 - yicF b3647 0.32 +1.0 3,818,225 - yicF b3647 0.42 -0.0 3,818,255 - yicF b3647 0.44 +0.5 3,818,255 - yicF b3647 0.44 +1.6 3,818,289 + yicF b3647 0.46 +1.0 3,818,304 + yicF b3647 0.47 +0.4 3,818,375 - yicF b3647 0.51 +0.5 3,818,375 - yicF b3647 0.51 +1.2 3,818,388 + yicF b3647 0.52 +0.0 3,818,390 + yicF b3647 0.52 -0.1 3,818,400 + yicF b3647 0.53 -0.5 3,818,400 + yicF b3647 0.53 +1.3 3,818,439 + yicF b3647 0.55 -0.3 3,818,496 + yicF b3647 0.59 -0.3 3,818,564 + yicF b3647 0.63 +1.5 3,818,627 + yicF b3647 0.66 -0.5 3,818,672 + yicF b3647 0.69 +0.1 3,818,672 + yicF b3647 0.69 -0.3 3,818,675 + yicF b3647 0.69 +0.2 3,818,680 - yicF b3647 0.69 +1.8 3,818,735 + yicF b3647 0.73 +0.0 3,818,743 - yicF b3647 0.73 -1.9 3,818,781 + yicF b3647 0.75 -0.0 3,818,794 + yicF b3647 0.76 -0.0 3,818,794 + yicF b3647 0.76 +0.6 3,818,806 + yicF b3647 0.77 +0.5 3,818,832 + yicF b3647 0.78 -1.2 3,818,840 - yicF b3647 0.79 -0.1 3,818,886 + yicF b3647 0.82 -0.2 3,818,899 + yicF b3647 0.82 -0.5 3,818,904 - yicF b3647 0.83 +1.9 3,818,920 + yicF b3647 0.84 +0.1 3,818,938 - yicF b3647 0.85 +0.5 3,818,994 - yicF b3647 0.88 +1.1 3,818,994 - yicF b3647 0.88 +1.2 3,819,005 - yicF b3647 0.89 +0.1 3,819,005 - yicF b3647 0.89 +0.6 3,819,041 - +0.2 3,819,053 - +0.5 3,819,065 - +0.8 3,819,111 - +0.8 3,819,121 + +0.3 3,819,121 + +3.3 3,819,130 - +1.8 3,819,173 - +0.7 3,819,191 + +0.2 3,819,197 - +0.1 3,819,197 - -0.4 3,819,212 - -1.1 3,819,234 + +0.1 3,819,252 - +0.4 3,819,252 - +0.1 3,819,303 - +1.3 3,819,394 - -1.1 3,819,394 - +0.1 3,820,087 - -0.0 3,820,111 - +0.5 3,820,144 + -0.9 3,820,144 + +0.5 3,820,151 + -0.1
Or see this region's nucleotide sequence