Strain Fitness in Escherichia coli BW25113 around b3224

Experiment: Spectinomycin 0.0125 mg/ml

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	Spectinomycin 0.0125 mg/ml
3,368,179	-	yhcI	b3222	0.78	+1.5
3,368,179	-	yhcI	b3222	0.78	+0.7
3,368,184	-	yhcI	b3222	0.78	+0.5
3,368,285	+				+0.6
3,368,327	-				+0.7
3,368,368	+				+0.2
3,368,585	+	nanE	b3223	0.31	-1.8
3,368,593	-	nanE	b3223	0.32	+0.6
3,368,696	-	nanE	b3223	0.47	+0.5
3,368,699	+	nanE	b3223	0.48	-0.9
3,368,699	+	nanE	b3223	0.48	+1.0
3,368,703	+	nanE	b3223	0.48	+0.8
3,368,768	+	nanE	b3223	0.58	+0.5
3,368,780	+	nanE	b3223	0.60	-0.6
3,368,791	+	nanE	b3223	0.61	-0.3
3,368,791	+	nanE	b3223	0.61	-0.2
3,368,887	+	nanE	b3223	0.75	+0.2
3,368,989	-	nanE	b3223	0.90	+0.4
3,368,989	-	nanE	b3223	0.90	+0.5
3,369,084	+				+0.2
3,369,297	+	nanT	b3224	0.13	-0.4
3,369,480	+	nanT	b3224	0.25	-0.9
3,369,488	-	nanT	b3224	0.26	-0.7
3,369,488	-	nanT	b3224	0.26	-0.9
3,369,538	-	nanT	b3224	0.29	+0.0
3,369,640	+	nanT	b3224	0.36	+0.7
3,369,669	+	nanT	b3224	0.38	-0.5
3,369,791	-	nanT	b3224	0.46	+0.4
3,369,793	+	nanT	b3224	0.46	+1.0
3,369,831	-	nanT	b3224	0.49	-1.4
3,369,941	+	nanT	b3224	0.56	-0.1
3,370,043	-	nanT	b3224	0.63	+0.4
3,370,071	+	nanT	b3224	0.65	+1.2
3,370,076	+	nanT	b3224	0.65	+0.8
3,370,108	-	nanT	b3224	0.67	+0.3
3,370,219	+	nanT	b3224	0.75	+0.2
3,370,219	+	nanT	b3224	0.75	+5.9
3,370,242	-	nanT	b3224	0.76	+0.6
3,370,243	+	nanT	b3224	0.76	+0.3
3,370,324	+	nanT	b3224	0.82	+0.7
3,370,343	-	nanT	b3224	0.83	+0.2
3,370,359	+	nanT	b3224	0.84	+0.4
3,370,407	+	nanT	b3224	0.87	+0.6
3,370,407	+	nanT	b3224	0.87	+1.1
3,370,470	+				-0.7
3,370,540	+				+0.2
3,370,553	-				-0.1
3,370,584	-				-0.5
3,370,584	-				-2.1
3,370,651	+				-0.4
3,370,732	+				+0.7
3,370,773	+				-1.1
3,370,773	+				+0.4
3,370,814	+	nanA	b3225	0.12	-0.2
3,370,814	+	nanA	b3225	0.12	+0.8
3,370,820	-	nanA	b3225	0.13	-0.8
3,370,937	-	nanA	b3225	0.26	+0.6
3,370,941	+	nanA	b3225	0.26	+0.6
3,370,941	+	nanA	b3225	0.26	-0.8
3,370,964	+	nanA	b3225	0.29	+0.9
3,370,964	+	nanA	b3225	0.29	-0.6
3,370,976	-	nanA	b3225	0.30	-0.4
3,371,002	+	nanA	b3225	0.33	+0.2
3,371,002	+	nanA	b3225	0.33	-0.1
3,371,043	-	nanA	b3225	0.38	+0.5
3,371,043	-	nanA	b3225	0.38	-0.1
3,371,112	+	nanA	b3225	0.46	-0.5
3,371,144	-	nanA	b3225	0.49	-0.8
3,371,193	-	nanA	b3225	0.55	-1.2
3,371,198	-	nanA	b3225	0.55	-0.0
3,371,255	+	nanA	b3225	0.62	+1.7
3,371,316	-	nanA	b3225	0.68	+0.6
3,371,316	-	nanA	b3225	0.68	-1.4
3,371,447	-	nanA	b3225	0.83	-0.6
3,371,454	-	nanA	b3225	0.84	-1.2
3,371,469	+	nanA	b3225	0.85	-0.7
3,371,469	+	nanA	b3225	0.85	+0.2
3,371,513	-				-0.4
3,371,571	+				-0.3
3,371,590	-				-0.1