Strain Fitness in Escherichia coli BW25113 around b3105

Experiment: Spectinomycin 0.0125 mg/ml

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	Spectinomycin 0.0125 mg/ml
3,250,390	-	yhaH	b3103	0.17	+0.3
3,250,390	-	yhaH	b3103	0.17	+1.4
3,250,414	+	yhaH	b3103	0.24	+0.6
3,250,427	-	yhaH	b3103	0.28	+0.4
3,250,533	+	yhaH	b3103	0.57	+0.8
3,250,533	+	yhaH	b3103	0.57	+0.3
3,250,533	+	yhaH	b3103	0.57	+0.9
3,250,533	+	yhaH	b3103	0.57	-1.2
3,250,558	-	yhaH	b3103	0.63	+0.1
3,250,558	-	yhaH	b3103	0.63	+0.3
3,250,564	+	yhaH	b3103	0.65	+0.4
3,250,569	+	yhaH	b3103	0.66	-0.5
3,250,571	+	yhaH	b3103	0.67	+0.4
3,250,571	+	yhaH	b3103	0.67	+0.4
3,250,576	+	yhaH	b3103	0.68	+0.8
3,250,577	-	yhaH	b3103	0.69	+0.7
3,250,578	+	yhaH	b3103	0.69	-0.8
3,250,578	+	yhaH	b3103	0.69	+0.3
3,250,583	+	yhaH	b3103	0.70	-0.1
3,250,623	+	yhaH	b3103	0.81	+0.4
3,250,623	+	yhaH	b3103	0.81	-1.5
3,250,640	+	yhaH	b3103	0.86	+1.4
3,250,640	+	yhaH	b3103	0.86	-0.9
3,250,645	+	yhaH	b3103	0.87	+0.5
3,250,672	+				+0.4
3,250,691	+				+0.0
3,250,712	+				+0.9
3,250,721	+				-0.5
3,250,841	+				+0.6
3,250,936	-				+0.7
3,250,982	-	yhaI	b3104	0.14	-1.2
3,250,982	-	yhaI	b3104	0.14	-0.2
3,250,987	-	yhaI	b3104	0.15	+0.0
3,250,996	-	yhaI	b3104	0.18	-0.0
3,250,996	-	yhaI	b3104	0.18	-1.9
3,251,004	-	yhaI	b3104	0.20	+0.3
3,251,004	-	yhaI	b3104	0.20	+1.1
3,251,006	-	yhaI	b3104	0.20	+0.2
3,251,016	+	yhaI	b3104	0.23	-1.5
3,251,024	-	yhaI	b3104	0.25	-0.1
3,251,044	-	yhaI	b3104	0.31	+0.6
3,251,058	+	yhaI	b3104	0.35	-0.7
3,251,083	-	yhaI	b3104	0.42	+0.8
3,251,083	-	yhaI	b3104	0.42	-0.6
3,251,085	+	yhaI	b3104	0.43	-0.7
3,251,120	+	yhaI	b3104	0.52	-0.7
3,251,122	-	yhaI	b3104	0.53	-0.1
3,251,122	-	yhaI	b3104	0.53	-0.6
3,251,124	+	yhaI	b3104	0.54	-0.9
3,251,136	-	yhaI	b3104	0.57	+1.1
3,251,136	-	yhaI	b3104	0.57	+0.1
3,251,140	+	yhaI	b3104	0.58	+2.7
3,251,141	+	yhaI	b3104	0.58	-0.5
3,251,142	+	yhaI	b3104	0.59	+0.1
3,251,142	+	yhaI	b3104	0.59	-0.5
3,251,142	+	yhaI	b3104	0.59	+0.3
3,251,145	+	yhaI	b3104	0.59	+0.7
3,251,145	+	yhaI	b3104	0.59	-0.6
3,251,153	-	yhaI	b3104	0.62	+0.6
3,251,160	+	yhaI	b3104	0.64	+0.2
3,251,188	+	yhaI	b3104	0.71	-1.5
3,251,256	+				-0.3
3,251,283	+				-0.5
3,251,338	-				-0.3
3,251,383	-				+1.3
3,251,396	-				+0.1
3,251,398	-				+1.1
3,251,398	-				-1.3
3,251,401	-				+0.2
3,251,401	-				+0.1
3,251,401	-				+0.7
3,251,401	-				+1.7
3,251,401	-				+1.0
3,251,401	-				+1.3
3,251,401	-				+1.1
3,251,402	-				+0.6
3,251,408	-				-0.2
3,251,408	-				+2.1
3,251,409	-				+0.8
3,251,409	-				-0.8
3,251,409	-				+0.1
3,251,409	-				-0.8
3,251,417	-				+1.5
3,251,423	-				+0.9
3,251,424	-				+2.8
3,251,452	-	yhaJ	b3105	0.12	-0.6
3,251,452	-	yhaJ	b3105	0.12	+1.6
3,251,452	-	yhaJ	b3105	0.12	+0.1
3,251,505	-	yhaJ	b3105	0.18	+1.5
3,251,505	-	yhaJ	b3105	0.18	+0.5
3,251,505	-	yhaJ	b3105	0.18	+0.8
3,251,517	-	yhaJ	b3105	0.20	+2.7
3,251,523	-	yhaJ	b3105	0.20	+0.4
3,251,549	+	yhaJ	b3105	0.23	-1.3
3,251,562	-	yhaJ	b3105	0.25	+1.1
3,251,562	-	yhaJ	b3105	0.25	-1.5
3,251,572	-	yhaJ	b3105	0.26	+0.3
3,251,572	-	yhaJ	b3105	0.26	-0.1
3,251,591	-	yhaJ	b3105	0.28	+0.2
3,251,598	+	yhaJ	b3105	0.29	+0.5
3,251,607	-	yhaJ	b3105	0.30	+0.5
3,251,607	-	yhaJ	b3105	0.30	-1.7
3,251,607	-	yhaJ	b3105	0.30	+0.5
3,251,620	-	yhaJ	b3105	0.31	+0.6
3,251,621	-	yhaJ	b3105	0.31	+1.7
3,251,621	-	yhaJ	b3105	0.31	+0.8
3,251,621	-	yhaJ	b3105	0.31	+0.4
3,251,631	-	yhaJ	b3105	0.32	+0.3
3,251,671	-	yhaJ	b3105	0.37	+0.4
3,251,700	+	yhaJ	b3105	0.40	-0.9
3,251,732	+	yhaJ	b3105	0.44	+0.7
3,251,736	-	yhaJ	b3105	0.44	+0.1
3,251,736	-	yhaJ	b3105	0.44	+0.5
3,251,736	-	yhaJ	b3105	0.44	+1.4
3,251,794	-	yhaJ	b3105	0.51	+0.1
3,251,923	-	yhaJ	b3105	0.65	-0.0
3,251,971	-	yhaJ	b3105	0.70	+0.7
3,251,988	-	yhaJ	b3105	0.72	-0.5
3,251,993	-	yhaJ	b3105	0.73	+1.2
3,252,027	-	yhaJ	b3105	0.77	-0.3
3,252,027	-	yhaJ	b3105	0.77	+1.2
3,252,031	-	yhaJ	b3105	0.77	+0.5
3,252,061	+	yhaJ	b3105	0.80	+0.7
3,252,061	+	yhaJ	b3105	0.80	+2.1
3,252,082	+	yhaJ	b3105	0.83	+0.7
3,252,082	+	yhaJ	b3105	0.83	+0.9
3,252,093	-	yhaJ	b3105	0.84	+0.5
3,252,115	+	yhaJ	b3105	0.86	+1.5
3,252,115	+	yhaJ	b3105	0.86	+0.5
3,252,115	+	yhaJ	b3105	0.86	+1.1
3,252,115	+	yhaJ	b3105	0.86	+0.1
3,252,115	+	yhaJ	b3105	0.86	+0.2
3,252,115	+	yhaJ	b3105	0.86	+0.8
3,252,123	-	yhaJ	b3105	0.87	+0.1
3,252,123	-	yhaJ	b3105	0.87	+1.1
3,252,197	+				-0.7
3,252,197	+				-1.5
3,252,281	-				-0.1
3,252,301	+				-0.8
3,252,310	-				-0.5
3,252,367	+				+0.7
3,252,375	-				-0.1
3,252,375	-				+1.1
3,252,380	+				-0.1
3,252,380	+				+1.1
3,252,411	-				-1.5
3,252,427	-	yhaK	b3106	0.12	+0.5
3,252,427	-	yhaK	b3106	0.12	+2.1
3,252,450	+	yhaK	b3106	0.16	+0.6
3,252,471	+	yhaK	b3106	0.19	-1.6
3,252,555	-	yhaK	b3106	0.30	-0.5
3,252,621	+	yhaK	b3106	0.40	+1.9
3,252,626	-	yhaK	b3106	0.41	-0.3
3,252,626	-	yhaK	b3106	0.41	-0.7
3,252,771	+	yhaK	b3106	0.61	+0.0
3,252,821	-	yhaK	b3106	0.68	-2.1
3,252,831	-	yhaK	b3106	0.70	+0.8
3,252,956	-	yhaK	b3106	0.88	+2.6
3,252,959	-	yhaK	b3106	0.88	+0.7
3,252,992	-				-1.5
3,253,158	-	yhaL	b3107	0.56	+0.5