Experiment: Spectinomycin 0.0125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yphF and yphG are separated by 96 nucleotides yphG and yphH are separated by 117 nucleotides
b2548: yphF - predicted sugar transporter subunit: periplasmic-binding component of ABC superfamily (NCBI), at 2,676,406 to 2,677,389
yphF
b2549: yphG - orf, hypothetical protein (VIMSS), at 2,677,486 to 2,680,767
yphG
b2550: yphH - putative NAGC-like transcriptional regulator (VIMSS), at 2,680,885 to 2,682,078
yphH
Position (kb)
2677
2678
2679
2680
2681 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2676.497 kb on - strand at 2676.507 kb on + strand, within yphF at 2676.568 kb on + strand, within yphF at 2676.603 kb on + strand, within yphF at 2676.603 kb on + strand, within yphF at 2676.623 kb on + strand, within yphF at 2676.693 kb on + strand, within yphF at 2676.693 kb on + strand, within yphF at 2676.701 kb on - strand, within yphF at 2676.701 kb on - strand, within yphF at 2676.790 kb on + strand, within yphF at 2676.856 kb on + strand, within yphF at 2676.856 kb on + strand, within yphF at 2676.858 kb on + strand, within yphF at 2676.858 kb on + strand, within yphF at 2676.868 kb on + strand, within yphF at 2676.899 kb on + strand, within yphF at 2676.955 kb on - strand, within yphF at 2676.979 kb on + strand, within yphF at 2677.020 kb on + strand, within yphF at 2677.033 kb on - strand, within yphF at 2677.033 kb on - strand, within yphF at 2677.059 kb on + strand, within yphF at 2677.061 kb on + strand, within yphF at 2677.079 kb on + strand, within yphF at 2677.079 kb on + strand, within yphF at 2677.084 kb on + strand, within yphF at 2677.084 kb on + strand, within yphF at 2677.113 kb on + strand, within yphF at 2677.127 kb on + strand, within yphF at 2677.127 kb on + strand, within yphF at 2677.127 kb on + strand, within yphF at 2677.138 kb on + strand, within yphF at 2677.152 kb on - strand, within yphF at 2677.152 kb on - strand, within yphF at 2677.172 kb on + strand, within yphF at 2677.172 kb on + strand, within yphF at 2677.190 kb on - strand, within yphF at 2677.201 kb on - strand, within yphF at 2677.239 kb on - strand, within yphF at 2677.240 kb on + strand, within yphF at 2677.242 kb on - strand, within yphF at 2677.246 kb on + strand, within yphF at 2677.246 kb on + strand, within yphF at 2677.248 kb on - strand, within yphF at 2677.251 kb on + strand, within yphF at 2677.251 kb on + strand, within yphF at 2677.254 kb on - strand, within yphF at 2677.256 kb on + strand, within yphF at 2677.256 kb on + strand, within yphF at 2677.259 kb on - strand, within yphF at 2677.259 kb on - strand, within yphF at 2677.261 kb on + strand, within yphF at 2677.264 kb on + strand, within yphF at 2677.264 kb on + strand, within yphF at 2677.277 kb on + strand, within yphF at 2677.279 kb on - strand, within yphF at 2677.285 kb on - strand, within yphF at 2677.286 kb on + strand, within yphF at 2677.287 kb on + strand, within yphF at 2677.294 kb on - strand at 2677.306 kb on + strand at 2677.306 kb on + strand at 2677.313 kb on + strand at 2677.313 kb on + strand at 2677.318 kb on + strand at 2677.323 kb on + strand at 2677.323 kb on + strand at 2677.323 kb on + strand at 2677.333 kb on + strand at 2677.333 kb on + strand at 2677.333 kb on + strand at 2677.333 kb on + strand at 2677.333 kb on + strand at 2677.333 kb on + strand at 2677.360 kb on + strand at 2677.386 kb on + strand at 2677.386 kb on + strand at 2677.387 kb on + strand at 2677.387 kb on + strand at 2677.388 kb on + strand at 2677.405 kb on - strand at 2677.405 kb on - strand at 2677.442 kb on - strand at 2677.482 kb on - strand at 2677.482 kb on - strand at 2677.485 kb on - strand at 2677.485 kb on - strand at 2677.544 kb on - strand at 2677.580 kb on + strand at 2677.588 kb on + strand at 2677.588 kb on - strand at 2677.593 kb on - strand at 2677.596 kb on - strand at 2677.605 kb on - strand at 2677.605 kb on - strand at 2677.608 kb on - strand at 2677.616 kb on - strand at 2677.631 kb on - strand at 2677.631 kb on - strand at 2677.661 kb on - strand at 2677.696 kb on - strand at 2677.696 kb on - strand at 2677.755 kb on - strand at 2677.898 kb on - strand, within yphG at 2677.937 kb on - strand, within yphG at 2678.041 kb on - strand, within yphG at 2678.041 kb on - strand, within yphG at 2678.073 kb on - strand, within yphG at 2678.073 kb on - strand, within yphG at 2678.144 kb on - strand, within yphG at 2678.144 kb on - strand, within yphG at 2678.161 kb on - strand, within yphG at 2678.179 kb on - strand, within yphG at 2678.542 kb on - strand, within yphG at 2678.542 kb on - strand, within yphG at 2678.554 kb on + strand, within yphG at 2678.562 kb on - strand, within yphG at 2678.770 kb on - strand, within yphG at 2678.770 kb on - strand, within yphG at 2678.842 kb on - strand, within yphG at 2678.842 kb on - strand, within yphG at 2678.865 kb on + strand, within yphG at 2678.866 kb on - strand, within yphG at 2678.955 kb on - strand, within yphG at 2679.045 kb on + strand, within yphG at 2679.070 kb on - strand, within yphG at 2679.210 kb on + strand, within yphG at 2679.210 kb on + strand, within yphG at 2679.349 kb on + strand, within yphG at 2679.357 kb on - strand, within yphG at 2679.357 kb on - strand, within yphG at 2679.357 kb on - strand, within yphG at 2679.384 kb on + strand, within yphG at 2679.384 kb on + strand, within yphG at 2679.549 kb on - strand, within yphG at 2679.549 kb on - strand, within yphG at 2679.567 kb on + strand, within yphG at 2679.585 kb on - strand, within yphG at 2679.634 kb on + strand, within yphG at 2679.711 kb on + strand, within yphG at 2679.870 kb on - strand, within yphG at 2680.055 kb on + strand, within yphG at 2680.083 kb on + strand, within yphG at 2680.346 kb on - strand, within yphG at 2680.346 kb on - strand, within yphG at 2680.348 kb on + strand, within yphG at 2680.403 kb on - strand, within yphG at 2680.459 kb on - strand at 2680.459 kb on - strand at 2680.626 kb on - strand at 2680.626 kb on - strand at 2680.788 kb on + strand at 2681.096 kb on + strand, within yphH at 2681.096 kb on + strand, within yphH at 2681.099 kb on + strand, within yphH at 2681.176 kb on + strand, within yphH at 2681.316 kb on - strand, within yphH at 2681.433 kb on + strand, within yphH at 2681.433 kb on + strand, within yphH at 2681.440 kb on + strand, within yphH at 2681.612 kb on - strand, within yphH at 2681.629 kb on + strand, within yphH at 2681.726 kb on - strand, within yphH
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.0125 mg/ml remove 2,676,497 - +1.5 2,676,507 + yphF b2548 0.10 -0.6 2,676,568 + yphF b2548 0.16 +0.1 2,676,603 + yphF b2548 0.20 +1.0 2,676,603 + yphF b2548 0.20 +0.1 2,676,623 + yphF b2548 0.22 -0.2 2,676,693 + yphF b2548 0.29 -1.0 2,676,693 + yphF b2548 0.29 +0.4 2,676,701 - yphF b2548 0.30 +0.6 2,676,701 - yphF b2548 0.30 -0.3 2,676,790 + yphF b2548 0.39 +2.0 2,676,856 + yphF b2548 0.46 +0.0 2,676,856 + yphF b2548 0.46 -1.2 2,676,858 + yphF b2548 0.46 +1.6 2,676,858 + yphF b2548 0.46 +0.6 2,676,868 + yphF b2548 0.47 +1.4 2,676,899 + yphF b2548 0.50 +0.8 2,676,955 - yphF b2548 0.56 -0.0 2,676,979 + yphF b2548 0.58 +0.5 2,677,020 + yphF b2548 0.62 -0.6 2,677,033 - yphF b2548 0.64 +0.3 2,677,033 - yphF b2548 0.64 -0.6 2,677,059 + yphF b2548 0.66 -1.5 2,677,061 + yphF b2548 0.67 -1.6 2,677,079 + yphF b2548 0.68 +0.3 2,677,079 + yphF b2548 0.68 +1.0 2,677,084 + yphF b2548 0.69 +1.5 2,677,084 + yphF b2548 0.69 -0.3 2,677,113 + yphF b2548 0.72 -1.3 2,677,127 + yphF b2548 0.73 -0.6 2,677,127 + yphF b2548 0.73 -0.0 2,677,127 + yphF b2548 0.73 +1.4 2,677,138 + yphF b2548 0.74 -0.6 2,677,152 - yphF b2548 0.76 -0.2 2,677,152 - yphF b2548 0.76 -0.6 2,677,172 + yphF b2548 0.78 -2.0 2,677,172 + yphF b2548 0.78 -0.2 2,677,190 - yphF b2548 0.80 -0.6 2,677,201 - yphF b2548 0.81 +0.5 2,677,239 - yphF b2548 0.85 -1.0 2,677,240 + yphF b2548 0.85 +0.6 2,677,242 - yphF b2548 0.85 -1.0 2,677,246 + yphF b2548 0.85 +0.6 2,677,246 + yphF b2548 0.85 +0.3 2,677,248 - yphF b2548 0.86 +0.4 2,677,251 + yphF b2548 0.86 -1.0 2,677,251 + yphF b2548 0.86 +0.2 2,677,254 - yphF b2548 0.86 +2.3 2,677,256 + yphF b2548 0.86 +0.4 2,677,256 + yphF b2548 0.86 +0.6 2,677,259 - yphF b2548 0.87 +0.4 2,677,259 - yphF b2548 0.87 -0.6 2,677,261 + yphF b2548 0.87 -0.2 2,677,264 + yphF b2548 0.87 -0.3 2,677,264 + yphF b2548 0.87 -1.3 2,677,277 + yphF b2548 0.89 +0.4 2,677,279 - yphF b2548 0.89 +0.3 2,677,285 - yphF b2548 0.89 +0.8 2,677,286 + yphF b2548 0.89 -0.2 2,677,287 + yphF b2548 0.90 +0.6 2,677,294 - -1.2 2,677,306 + +0.6 2,677,306 + +0.6 2,677,313 + +0.1 2,677,313 + +0.3 2,677,318 + +1.0 2,677,323 + +1.3 2,677,323 + -0.0 2,677,323 + +0.4 2,677,333 + -1.1 2,677,333 + -0.6 2,677,333 + -0.5 2,677,333 + -1.0 2,677,333 + +0.5 2,677,333 + -0.5 2,677,360 + +1.0 2,677,386 + +1.6 2,677,386 + +0.6 2,677,387 + +0.8 2,677,387 + -0.0 2,677,388 + -0.7 2,677,405 - +0.1 2,677,405 - -0.5 2,677,442 - -0.3 2,677,482 - -0.6 2,677,482 - +0.6 2,677,485 - -0.3 2,677,485 - -0.6 2,677,544 - -0.7 2,677,580 + -1.8 2,677,588 + +1.0 2,677,588 - +1.3 2,677,593 - -0.4 2,677,596 - -0.4 2,677,605 - +0.7 2,677,605 - +0.7 2,677,608 - +2.2 2,677,616 - -0.2 2,677,631 - -0.7 2,677,631 - +2.0 2,677,661 - +0.1 2,677,696 - +1.8 2,677,696 - -2.2 2,677,755 - +0.5 2,677,898 - yphG b2549 0.13 +1.8 2,677,937 - yphG b2549 0.14 -0.1 2,678,041 - yphG b2549 0.17 +0.4 2,678,041 - yphG b2549 0.17 +0.2 2,678,073 - yphG b2549 0.18 +0.2 2,678,073 - yphG b2549 0.18 -0.8 2,678,144 - yphG b2549 0.20 -0.1 2,678,144 - yphG b2549 0.20 -0.2 2,678,161 - yphG b2549 0.21 -0.8 2,678,179 - yphG b2549 0.21 -1.0 2,678,542 - yphG b2549 0.32 -1.0 2,678,542 - yphG b2549 0.32 -0.2 2,678,554 + yphG b2549 0.33 +0.4 2,678,562 - yphG b2549 0.33 -1.5 2,678,770 - yphG b2549 0.39 +0.3 2,678,770 - yphG b2549 0.39 +1.0 2,678,842 - yphG b2549 0.41 +0.1 2,678,842 - yphG b2549 0.41 +0.3 2,678,865 + yphG b2549 0.42 -0.1 2,678,866 - yphG b2549 0.42 -0.6 2,678,955 - yphG b2549 0.45 -0.0 2,679,045 + yphG b2549 0.48 +1.0 2,679,070 - yphG b2549 0.48 +0.2 2,679,210 + yphG b2549 0.53 -0.0 2,679,210 + yphG b2549 0.53 -1.0 2,679,349 + yphG b2549 0.57 +0.6 2,679,357 - yphG b2549 0.57 -0.0 2,679,357 - yphG b2549 0.57 -0.0 2,679,357 - yphG b2549 0.57 +1.0 2,679,384 + yphG b2549 0.58 +0.1 2,679,384 + yphG b2549 0.58 -0.5 2,679,549 - yphG b2549 0.63 +0.1 2,679,549 - yphG b2549 0.63 -0.9 2,679,567 + yphG b2549 0.63 -0.0 2,679,585 - yphG b2549 0.64 -0.3 2,679,634 + yphG b2549 0.65 +0.5 2,679,711 + yphG b2549 0.68 -0.7 2,679,870 - yphG b2549 0.73 -1.4 2,680,055 + yphG b2549 0.78 -1.5 2,680,083 + yphG b2549 0.79 -0.1 2,680,346 - yphG b2549 0.87 +0.1 2,680,346 - yphG b2549 0.87 -0.4 2,680,348 + yphG b2549 0.87 -1.0 2,680,403 - yphG b2549 0.89 +2.6 2,680,459 - -0.1 2,680,459 - -0.4 2,680,626 - +0.7 2,680,626 - -0.3 2,680,788 + +0.6 2,681,096 + yphH b2550 0.18 +0.4 2,681,096 + yphH b2550 0.18 -0.3 2,681,099 + yphH b2550 0.18 -0.6 2,681,176 + yphH b2550 0.24 +0.7 2,681,316 - yphH b2550 0.36 +1.2 2,681,433 + yphH b2550 0.46 +0.2 2,681,433 + yphH b2550 0.46 +0.3 2,681,440 + yphH b2550 0.46 +0.7 2,681,612 - yphH b2550 0.61 +0.3 2,681,629 + yphH b2550 0.62 +0.2 2,681,726 - yphH b2550 0.70 +1.0
Or see this region's nucleotide sequence