Experiment: Spectinomycin 0.0125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ptrB and yebE are separated by 208 nucleotides yebE and yebF are separated by 326 nucleotides yebF and yebG are separated by 66 nucleotides
b1845: ptrB - protease II (NCBI), at 1,924,803 to 1,926,863
ptrB
b1846: yebE - hypothetical protein (NCBI), at 1,927,072 to 1,927,731
yebE
b1847: yebF - predicted protein (RefSeq), at 1,928,058 to 1,928,414
yebF
b1848: yebG - conserved protein regulated by LexA (NCBI), at 1,928,481 to 1,928,771
yebG
Position (kb)
1927
1928 Strain fitness (log2 ratio)
-1
0
1
2 at 1926.172 kb on + strand, within ptrB at 1926.172 kb on + strand, within ptrB at 1926.326 kb on - strand, within ptrB at 1926.403 kb on - strand, within ptrB at 1926.489 kb on - strand, within ptrB at 1926.492 kb on + strand, within ptrB at 1926.492 kb on + strand, within ptrB at 1926.531 kb on + strand, within ptrB at 1926.554 kb on - strand, within ptrB at 1926.554 kb on - strand, within ptrB at 1926.593 kb on + strand, within ptrB at 1926.750 kb on + strand at 1926.750 kb on + strand at 1927.234 kb on + strand, within yebE at 1927.234 kb on + strand, within yebE at 1927.267 kb on + strand, within yebE at 1927.334 kb on - strand, within yebE at 1927.382 kb on - strand, within yebE at 1927.382 kb on - strand, within yebE at 1927.407 kb on + strand, within yebE at 1927.533 kb on + strand, within yebE at 1927.578 kb on + strand, within yebE at 1927.767 kb on - strand at 1927.861 kb on + strand at 1927.896 kb on + strand at 1928.014 kb on - strand at 1928.054 kb on + strand at 1928.054 kb on + strand at 1928.324 kb on + strand, within yebF at 1928.324 kb on + strand, within yebF at 1928.426 kb on + strand at 1928.426 kb on + strand at 1928.449 kb on - strand at 1928.449 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.0125 mg/ml remove 1,926,172 + ptrB b1845 0.66 +1.6 1,926,172 + ptrB b1845 0.66 +0.1 1,926,326 - ptrB b1845 0.74 -0.5 1,926,403 - ptrB b1845 0.78 +0.5 1,926,489 - ptrB b1845 0.82 -0.2 1,926,492 + ptrB b1845 0.82 -0.6 1,926,492 + ptrB b1845 0.82 +1.3 1,926,531 + ptrB b1845 0.84 -0.2 1,926,554 - ptrB b1845 0.85 +0.3 1,926,554 - ptrB b1845 0.85 -0.8 1,926,593 + ptrB b1845 0.87 -0.6 1,926,750 + -0.5 1,926,750 + +2.2 1,927,234 + yebE b1846 0.25 +1.1 1,927,234 + yebE b1846 0.25 +1.3 1,927,267 + yebE b1846 0.30 -0.2 1,927,334 - yebE b1846 0.40 +1.1 1,927,382 - yebE b1846 0.47 +0.4 1,927,382 - yebE b1846 0.47 +0.8 1,927,407 + yebE b1846 0.51 +0.6 1,927,533 + yebE b1846 0.70 +0.4 1,927,578 + yebE b1846 0.77 +1.1 1,927,767 - +0.5 1,927,861 + -0.2 1,927,896 + +1.6 1,928,014 - +1.2 1,928,054 + -1.7 1,928,054 + +0.1 1,928,324 + yebF b1847 0.75 +0.5 1,928,324 + yebF b1847 0.75 -0.9 1,928,426 + -0.1 1,928,426 + -0.2 1,928,449 - +0.3 1,928,449 - -1.0
Or see this region's nucleotide sequence