Experiment: Spectinomycin 0.0125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ynbC and ynbD are separated by 13 nucleotides ynbD and azoR are separated by 53 nucleotides azoR and hrpA are separated by 200 nucleotides
b1410: ynbC - predicted hydrolase (NCBI), at 1,477,162 to 1,478,919
ynbC
b1411: ynbD - predicted phosphatase, inner membrane protein (NCBI), at 1,478,933 to 1,480,225
ynbD
b1412: azoR - acyl carrier protein phosphodiesterase (NCBI), at 1,480,279 to 1,480,884
azoR
b1413: hrpA - ATP-dependent helicase (RefSeq), at 1,481,085 to 1,484,987
hrpA
Position (kb)
1478
1479
1480
1481 Strain fitness (log2 ratio)
-1
0
1
2
3 at 1478.058 kb on + strand, within ynbC at 1478.302 kb on - strand, within ynbC at 1478.317 kb on - strand, within ynbC at 1478.327 kb on + strand, within ynbC at 1478.410 kb on + strand, within ynbC at 1479.031 kb on - strand at 1479.098 kb on + strand, within ynbD at 1479.163 kb on + strand, within ynbD at 1479.431 kb on - strand, within ynbD at 1479.489 kb on + strand, within ynbD at 1479.501 kb on + strand, within ynbD at 1479.504 kb on + strand, within ynbD at 1479.574 kb on - strand, within ynbD at 1479.579 kb on - strand, within ynbD at 1479.579 kb on - strand, within ynbD at 1479.586 kb on - strand, within ynbD at 1479.670 kb on - strand, within ynbD at 1479.673 kb on + strand, within ynbD at 1479.970 kb on - strand, within ynbD at 1480.154 kb on - strand at 1480.167 kb on + strand at 1480.271 kb on + strand at 1480.271 kb on + strand at 1480.667 kb on - strand, within azoR at 1480.729 kb on + strand, within azoR at 1480.729 kb on + strand, within azoR
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.0125 mg/ml remove 1,478,058 + ynbC b1410 0.51 +1.7 1,478,302 - ynbC b1410 0.65 -0.0 1,478,317 - ynbC b1410 0.66 +0.3 1,478,327 + ynbC b1410 0.66 +1.2 1,478,410 + ynbC b1410 0.71 +1.2 1,479,031 - +0.6 1,479,098 + ynbD b1411 0.13 +3.3 1,479,163 + ynbD b1411 0.18 +1.5 1,479,431 - ynbD b1411 0.39 +1.4 1,479,489 + ynbD b1411 0.43 +1.6 1,479,501 + ynbD b1411 0.44 +2.4 1,479,504 + ynbD b1411 0.44 +2.6 1,479,574 - ynbD b1411 0.50 +0.2 1,479,579 - ynbD b1411 0.50 -0.8 1,479,579 - ynbD b1411 0.50 -0.1 1,479,586 - ynbD b1411 0.51 -0.7 1,479,670 - ynbD b1411 0.57 -0.8 1,479,673 + ynbD b1411 0.57 +1.1 1,479,970 - ynbD b1411 0.80 +0.4 1,480,154 - +0.1 1,480,167 + +2.6 1,480,271 + +3.1 1,480,271 + +3.1 1,480,667 - azoR b1412 0.64 +1.4 1,480,729 + azoR b1412 0.74 +2.3 1,480,729 + azoR b1412 0.74 +0.8
Or see this region's nucleotide sequence