Experiment: Spectinomycin 0.0125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yliI and yliJ overlap by 4 nucleotides yliJ and dacC are separated by 246 nucleotides
b0837: yliI - predicted dehydrogenase (NCBI), at 877,965 to 879,080
yliI
b0838: yliJ - putative transferase (VIMSS), at 879,077 to 879,703
yliJ
b0839: dacC - D-alanyl-D-alanine carboxypeptidase (penicillin-binding protein 6a) (NCBI), at 879,950 to 881,152
dacC
Position (kb) 879
880 Strain fitness (log2 ratio) -1
0
1
2 at 878.117 kb on - strand, within yliI at 878.117 kb on - strand, within yliI at 878.310 kb on + strand, within yliI at 878.318 kb on - strand, within yliI at 878.318 kb on - strand, within yliI at 878.617 kb on + strand, within yliI at 878.644 kb on + strand, within yliI at 878.644 kb on + strand, within yliI at 878.654 kb on - strand, within yliI at 878.786 kb on + strand, within yliI at 878.847 kb on - strand, within yliI at 878.847 kb on - strand, within yliI at 878.848 kb on + strand, within yliI at 879.008 kb on + strand at 879.026 kb on + strand at 879.046 kb on - strand at 879.046 kb on - strand at 879.084 kb on - strand at 879.092 kb on + strand at 879.138 kb on - strand at 879.167 kb on + strand, within yliJ at 879.170 kb on - strand, within yliJ at 879.226 kb on - strand, within yliJ at 879.226 kb on - strand, within yliJ at 879.353 kb on - strand, within yliJ at 879.353 kb on - strand, within yliJ at 879.523 kb on - strand, within yliJ at 879.525 kb on - strand, within yliJ at 879.599 kb on - strand, within yliJ at 879.599 kb on - strand, within yliJ at 879.613 kb on - strand, within yliJ at 879.637 kb on + strand, within yliJ at 879.680 kb on + strand at 879.703 kb on + strand at 879.727 kb on + strand at 879.727 kb on + strand at 879.741 kb on - strand at 879.742 kb on - strand at 879.817 kb on - strand at 879.961 kb on - strand at 879.963 kb on + strand at 880.059 kb on - strand at 880.156 kb on + strand, within dacC at 880.170 kb on - strand, within dacC at 880.270 kb on - strand, within dacC at 880.298 kb on + strand, within dacC at 880.298 kb on + strand, within dacC at 880.306 kb on + strand, within dacC at 880.453 kb on + strand, within dacC at 880.472 kb on - strand, within dacC at 880.489 kb on + strand, within dacC at 880.524 kb on - strand, within dacC at 880.550 kb on + strand, within dacC at 880.550 kb on + strand, within dacC at 880.568 kb on + strand, within dacC at 880.676 kb on + strand, within dacC
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.0125 mg/ml remove 878,117 - yliI b0837 0.14 -0.4 878,117 - yliI b0837 0.14 -1.1 878,310 + yliI b0837 0.31 +0.5 878,318 - yliI b0837 0.32 -0.5 878,318 - yliI b0837 0.32 +1.1 878,617 + yliI b0837 0.58 -1.1 878,644 + yliI b0837 0.61 -0.3 878,644 + yliI b0837 0.61 +0.5 878,654 - yliI b0837 0.62 +0.4 878,786 + yliI b0837 0.74 +0.8 878,847 - yliI b0837 0.79 -0.3 878,847 - yliI b0837 0.79 +0.6 878,848 + yliI b0837 0.79 -0.5 879,008 + +0.4 879,026 + -0.3 879,046 - +0.1 879,046 - +0.4 879,084 - +0.9 879,092 + -0.9 879,138 - +0.4 879,167 + yliJ b0838 0.14 +1.1 879,170 - yliJ b0838 0.15 +1.2 879,226 - yliJ b0838 0.24 +0.3 879,226 - yliJ b0838 0.24 +0.9 879,353 - yliJ b0838 0.44 -0.1 879,353 - yliJ b0838 0.44 -0.6 879,523 - yliJ b0838 0.71 +0.8 879,525 - yliJ b0838 0.71 +0.4 879,599 - yliJ b0838 0.83 +0.7 879,599 - yliJ b0838 0.83 +0.9 879,613 - yliJ b0838 0.85 +1.1 879,637 + yliJ b0838 0.89 +1.2 879,680 + -0.6 879,703 + -0.4 879,727 + +0.2 879,727 + +0.9 879,741 - -0.4 879,742 - +0.7 879,817 - +0.9 879,961 - -1.0 879,963 + -0.1 880,059 - -0.9 880,156 + dacC b0839 0.17 +0.3 880,170 - dacC b0839 0.18 -0.5 880,270 - dacC b0839 0.27 -0.6 880,298 + dacC b0839 0.29 -0.8 880,298 + dacC b0839 0.29 +0.7 880,306 + dacC b0839 0.30 +0.0 880,453 + dacC b0839 0.42 +0.8 880,472 - dacC b0839 0.43 -0.8 880,489 + dacC b0839 0.45 -1.1 880,524 - dacC b0839 0.48 +0.4 880,550 + dacC b0839 0.50 -0.1 880,550 + dacC b0839 0.50 +0.4 880,568 + dacC b0839 0.51 +0.9 880,676 + dacC b0839 0.60 +1.9
Or see this region's nucleotide sequence