Experiment: Spectinomycin 0.0125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt modC and ybhA are separated by 0 nucleotides ybhA and ybhE are separated by 154 nucleotides ybhE and ybhD are separated by 40 nucleotides
b0765: modC - molybdate transporter subunit (NCBI), at 795,777 to 796,835
modC
b0766: ybhA - predicted hydrolase (NCBI), at 796,836 to 797,654
ybhA
b0767: ybhE - 6-phosphogluconolactonase (NCBI), at 797,809 to 798,804
ybhE
b0768: ybhD - predicted DNA-binding transcriptional regulator (RefSeq), at 798,845 to 799,798
ybhD
Position (kb) 797
798
799 Strain fitness (log2 ratio) -2
-1
0
1
2 at 796.862 kb on + strand at 797.012 kb on - strand, within ybhA at 797.022 kb on - strand, within ybhA at 797.100 kb on - strand, within ybhA at 797.122 kb on - strand, within ybhA at 797.289 kb on + strand, within ybhA at 797.318 kb on - strand, within ybhA at 797.334 kb on + strand, within ybhA at 797.334 kb on + strand at 797.380 kb on - strand, within ybhA at 797.565 kb on + strand, within ybhA at 797.565 kb on + strand, within ybhA at 797.592 kb on - strand at 797.615 kb on - strand at 797.615 kb on - strand at 797.702 kb on + strand at 797.702 kb on + strand at 797.775 kb on + strand at 797.960 kb on + strand, within ybhE at 798.019 kb on + strand, within ybhE at 798.080 kb on + strand, within ybhE at 798.105 kb on - strand, within ybhE at 798.134 kb on - strand at 798.207 kb on + strand, within ybhE at 798.207 kb on + strand, within ybhE at 798.414 kb on - strand, within ybhE at 798.414 kb on - strand, within ybhE at 798.502 kb on + strand, within ybhE at 798.510 kb on - strand, within ybhE at 798.533 kb on + strand, within ybhE at 798.533 kb on + strand, within ybhE at 798.533 kb on + strand, within ybhE at 798.660 kb on - strand at 798.736 kb on - strand at 798.860 kb on - strand at 798.860 kb on - strand at 799.113 kb on - strand, within ybhD at 799.113 kb on - strand, within ybhD at 799.171 kb on + strand, within ybhD at 799.191 kb on + strand, within ybhD at 799.191 kb on + strand, within ybhD at 799.297 kb on - strand, within ybhD at 799.301 kb on - strand, within ybhD at 799.301 kb on - strand, within ybhD at 799.313 kb on - strand, within ybhD at 799.313 kb on - strand, within ybhD at 799.313 kb on - strand, within ybhD at 799.315 kb on + strand, within ybhD at 799.323 kb on - strand, within ybhD at 799.362 kb on - strand, within ybhD at 799.384 kb on + strand, within ybhD at 799.435 kb on + strand, within ybhD at 799.437 kb on + strand, within ybhD at 799.652 kb on + strand, within ybhD
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.0125 mg/ml remove 796,862 + +0.9 797,012 - ybhA b0766 0.21 -0.1 797,022 - ybhA b0766 0.23 -1.3 797,100 - ybhA b0766 0.32 -0.1 797,122 - ybhA b0766 0.35 +0.4 797,289 + ybhA b0766 0.55 -2.5 797,318 - ybhA b0766 0.59 -0.3 797,334 + ybhA b0766 0.61 +0.3 797,334 + +2.5 797,380 - ybhA b0766 0.66 -2.1 797,565 + ybhA b0766 0.89 +0.2 797,565 + ybhA b0766 0.89 -0.5 797,592 - +0.5 797,615 - +1.3 797,615 - +0.2 797,702 + +0.9 797,702 + -0.1 797,775 + -0.2 797,960 + ybhE b0767 0.15 +0.3 798,019 + ybhE b0767 0.21 -1.1 798,080 + ybhE b0767 0.27 -1.4 798,105 - ybhE b0767 0.30 -2.3 798,134 - +1.9 798,207 + ybhE b0767 0.40 +0.9 798,207 + ybhE b0767 0.40 -1.7 798,414 - ybhE b0767 0.61 -0.9 798,414 - ybhE b0767 0.61 -1.4 798,502 + ybhE b0767 0.70 -2.2 798,510 - ybhE b0767 0.70 -0.1 798,533 + ybhE b0767 0.73 -0.7 798,533 + ybhE b0767 0.73 -2.7 798,533 + ybhE b0767 0.73 -1.4 798,660 - +0.9 798,736 - +0.9 798,860 - +0.3 798,860 - +1.8 799,113 - ybhD b0768 0.28 +0.4 799,113 - ybhD b0768 0.28 -1.4 799,171 + ybhD b0768 0.34 -0.1 799,191 + ybhD b0768 0.36 -0.8 799,191 + ybhD b0768 0.36 +0.1 799,297 - ybhD b0768 0.47 +0.8 799,301 - ybhD b0768 0.48 -0.6 799,301 - ybhD b0768 0.48 +0.9 799,313 - ybhD b0768 0.49 +0.4 799,313 - ybhD b0768 0.49 +0.8 799,313 - ybhD b0768 0.49 -0.3 799,315 + ybhD b0768 0.49 +0.1 799,323 - ybhD b0768 0.50 -0.2 799,362 - ybhD b0768 0.54 -0.1 799,384 + ybhD b0768 0.56 +1.1 799,435 + ybhD b0768 0.62 -0.2 799,437 + ybhD b0768 0.62 +1.2 799,652 + ybhD b0768 0.85 -0.4
Or see this region's nucleotide sequence