Experiment: Spectinomycin 0.0125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt modA and modB overlap by 1 nucleotides modB and modC are separated by 2 nucleotides modC and ybhA are separated by 0 nucleotides ybhA and ybhE are separated by 154 nucleotides
b0763: modA - molybdate transporter subunit (NCBI), at 794,312 to 795,085
modA
b0764: modB - molybdate ABC transporter permease protein (NCBI), at 795,085 to 795,774
modB
b0765: modC - molybdate transporter subunit (NCBI), at 795,777 to 796,835
modC
b0766: ybhA - predicted hydrolase (NCBI), at 796,836 to 797,654
ybhA
b0767: ybhE - 6-phosphogluconolactonase (NCBI), at 797,809 to 798,804
ybhE
Position (kb) 795
796
797 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 794.800 kb on + strand, within modA at 794.876 kb on + strand, within modA at 794.989 kb on - strand, within modA at 795.088 kb on + strand at 795.113 kb on - strand at 795.153 kb on + strand at 795.230 kb on + strand, within modB at 795.330 kb on - strand, within modB at 795.549 kb on + strand, within modB at 795.549 kb on + strand at 795.552 kb on - strand, within modB at 795.638 kb on + strand, within modB at 795.638 kb on + strand, within modB at 795.642 kb on - strand, within modB at 795.644 kb on + strand, within modB at 795.646 kb on - strand, within modB at 795.695 kb on - strand, within modB at 795.811 kb on + strand at 795.811 kb on + strand at 795.820 kb on - strand at 795.820 kb on - strand at 796.082 kb on + strand, within modC at 796.082 kb on + strand, within modC at 796.218 kb on + strand, within modC at 796.234 kb on + strand, within modC at 796.234 kb on + strand, within modC at 796.352 kb on + strand, within modC at 796.369 kb on + strand, within modC at 796.369 kb on + strand, within modC at 796.377 kb on + strand, within modC at 796.377 kb on + strand, within modC at 796.377 kb on + strand, within modC at 796.377 kb on - strand, within modC at 796.377 kb on - strand, within modC at 796.590 kb on - strand, within modC at 796.862 kb on + strand at 797.012 kb on - strand, within ybhA at 797.022 kb on - strand, within ybhA at 797.100 kb on - strand, within ybhA at 797.122 kb on - strand, within ybhA at 797.289 kb on + strand, within ybhA at 797.318 kb on - strand, within ybhA at 797.334 kb on + strand, within ybhA at 797.334 kb on + strand at 797.380 kb on - strand, within ybhA at 797.565 kb on + strand, within ybhA at 797.565 kb on + strand, within ybhA at 797.592 kb on - strand at 797.615 kb on - strand at 797.615 kb on - strand at 797.702 kb on + strand at 797.702 kb on + strand at 797.775 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.0125 mg/ml remove 794,800 + modA b0763 0.63 +2.8 794,876 + modA b0763 0.73 +2.6 794,989 - modA b0763 0.87 +2.9 795,088 + +0.1 795,113 - +2.7 795,153 + +2.4 795,230 + modB b0764 0.21 +2.0 795,330 - modB b0764 0.36 +1.5 795,549 + modB b0764 0.67 +0.7 795,549 + -0.1 795,552 - modB b0764 0.68 +3.2 795,638 + modB b0764 0.80 +2.1 795,638 + modB b0764 0.80 +1.3 795,642 - modB b0764 0.81 +2.3 795,644 + modB b0764 0.81 +2.6 795,646 - modB b0764 0.81 +2.9 795,695 - modB b0764 0.88 +2.1 795,811 + +3.0 795,811 + +0.3 795,820 - +1.9 795,820 - +1.4 796,082 + modC b0765 0.29 +2.9 796,082 + modC b0765 0.29 +1.2 796,218 + modC b0765 0.42 +3.7 796,234 + modC b0765 0.43 +2.6 796,234 + modC b0765 0.43 +0.7 796,352 + modC b0765 0.54 +1.2 796,369 + modC b0765 0.56 +3.5 796,369 + modC b0765 0.56 +1.3 796,377 + modC b0765 0.57 +1.8 796,377 + modC b0765 0.57 +2.5 796,377 + modC b0765 0.57 +1.5 796,377 - modC b0765 0.57 +1.8 796,377 - modC b0765 0.57 +1.6 796,590 - modC b0765 0.77 +0.6 796,862 + +0.9 797,012 - ybhA b0766 0.21 -0.1 797,022 - ybhA b0766 0.23 -1.3 797,100 - ybhA b0766 0.32 -0.1 797,122 - ybhA b0766 0.35 +0.4 797,289 + ybhA b0766 0.55 -2.5 797,318 - ybhA b0766 0.59 -0.3 797,334 + ybhA b0766 0.61 +0.3 797,334 + +2.5 797,380 - ybhA b0766 0.66 -2.1 797,565 + ybhA b0766 0.89 +0.2 797,565 + ybhA b0766 0.89 -0.5 797,592 - +0.5 797,615 - +1.3 797,615 - +0.2 797,702 + +0.9 797,702 + -0.1 797,775 + -0.2
Or see this region's nucleotide sequence