Experiment: Spectinomycin 0.0125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt galM and galK overlap by 7 nucleotides galK and galT are separated by 3 nucleotides
b0756: galM - galactose-1-epimerase (mutarotase) (NCBI), at 787,020 to 788,060
galM
b0757: galK - galactokinase GalK (EC 2.7.1.6) <small>(from data)</small>, at 788,054 to 789,202
galK
b0758: galT - galactose-1-phosphate uridylyltransferase (NCBI), at 789,206 to 790,252
galT
Position (kb) 788
789
790 Strain fitness (log2 ratio) -2
-1
0
1 at 787.080 kb on - strand at 787.087 kb on + strand at 787.091 kb on - strand at 787.106 kb on + strand at 787.172 kb on - strand, within galM at 787.307 kb on - strand, within galM at 787.308 kb on - strand, within galM at 787.414 kb on - strand, within galM at 787.510 kb on - strand, within galM at 787.569 kb on - strand, within galM at 787.749 kb on + strand, within galM at 787.826 kb on + strand, within galM at 787.876 kb on - strand, within galM at 787.962 kb on - strand at 787.962 kb on - strand at 787.985 kb on + strand at 788.062 kb on + strand at 788.062 kb on + strand at 788.065 kb on - strand at 788.252 kb on + strand, within galK at 788.406 kb on - strand, within galK at 788.406 kb on - strand, within galK at 788.436 kb on - strand, within galK at 788.479 kb on + strand, within galK at 788.479 kb on + strand, within galK at 788.487 kb on - strand, within galK at 788.487 kb on - strand, within galK at 788.518 kb on - strand, within galK at 788.531 kb on + strand, within galK at 788.531 kb on + strand, within galK at 788.531 kb on + strand, within galK at 788.531 kb on + strand, within galK at 788.600 kb on - strand, within galK at 788.610 kb on + strand, within galK at 788.644 kb on - strand, within galK at 788.648 kb on - strand, within galK at 788.732 kb on + strand, within galK at 788.829 kb on + strand, within galK at 788.832 kb on - strand, within galK at 789.031 kb on + strand, within galK at 789.039 kb on - strand, within galK at 789.050 kb on + strand, within galK at 789.063 kb on + strand, within galK at 789.063 kb on + strand, within galK at 789.241 kb on - strand at 789.338 kb on - strand, within galT at 789.338 kb on - strand, within galT at 789.425 kb on - strand, within galT at 789.425 kb on - strand at 789.433 kb on - strand, within galT at 789.581 kb on - strand, within galT at 789.661 kb on + strand, within galT at 789.712 kb on - strand, within galT at 789.712 kb on - strand, within galT at 789.774 kb on - strand, within galT at 789.811 kb on + strand, within galT at 789.908 kb on + strand, within galT at 790.161 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.0125 mg/ml remove 787,080 - +0.2 787,087 + +0.4 787,091 - +0.8 787,106 + -0.7 787,172 - galM b0756 0.15 -0.4 787,307 - galM b0756 0.28 -0.3 787,308 - galM b0756 0.28 +1.3 787,414 - galM b0756 0.38 -1.3 787,510 - galM b0756 0.47 -0.1 787,569 - galM b0756 0.53 -0.5 787,749 + galM b0756 0.70 +0.1 787,826 + galM b0756 0.77 -1.4 787,876 - galM b0756 0.82 +0.5 787,962 - -1.2 787,962 - -0.7 787,985 + +0.4 788,062 + -2.1 788,062 + +0.9 788,065 - +0.2 788,252 + galK b0757 0.17 +0.8 788,406 - galK b0757 0.31 +0.9 788,406 - galK b0757 0.31 -0.7 788,436 - galK b0757 0.33 -0.3 788,479 + galK b0757 0.37 -2.1 788,479 + galK b0757 0.37 -1.9 788,487 - galK b0757 0.38 +1.5 788,487 - galK b0757 0.38 +0.7 788,518 - galK b0757 0.40 -0.4 788,531 + galK b0757 0.42 -0.7 788,531 + galK b0757 0.42 -0.8 788,531 + galK b0757 0.42 -1.4 788,531 + galK b0757 0.42 -0.6 788,600 - galK b0757 0.48 -0.7 788,610 + galK b0757 0.48 +0.9 788,644 - galK b0757 0.51 -1.2 788,648 - galK b0757 0.52 +0.5 788,732 + galK b0757 0.59 -0.3 788,829 + galK b0757 0.67 +1.1 788,832 - galK b0757 0.68 +0.3 789,031 + galK b0757 0.85 +0.1 789,039 - galK b0757 0.86 -1.4 789,050 + galK b0757 0.87 +0.2 789,063 + galK b0757 0.88 -1.1 789,063 + galK b0757 0.88 -1.3 789,241 - -0.5 789,338 - galT b0758 0.13 -1.1 789,338 - galT b0758 0.13 -1.4 789,425 - galT b0758 0.21 -0.4 789,425 - +0.5 789,433 - galT b0758 0.22 -0.4 789,581 - galT b0758 0.36 -0.7 789,661 + galT b0758 0.43 +0.8 789,712 - galT b0758 0.48 +0.6 789,712 - galT b0758 0.48 -0.4 789,774 - galT b0758 0.54 -0.8 789,811 + galT b0758 0.58 -0.5 789,908 + galT b0758 0.67 +0.6 790,161 - +0.5
Or see this region's nucleotide sequence