Experiment: Spectinomycin 0.0125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt cyoE and cyoD are separated by 11 nucleotides cyoD and cyoC overlap by 1 nucleotides cyoC and cyoB overlap by 11 nucleotides cyoB and cyoA are separated by 21 nucleotides
b0428: cyoE - protoheme IX farnesyltransferase (NCBI), at 446,039 to 446,929
cyoE
b0429: cyoD - cytochrome o ubiquinol oxidase subunit IV (NCBI), at 446,941 to 447,270
cyoD
b0430: cyoC - cytochrome o ubiquinol oxidase subunit III (NCBI), at 447,270 to 447,884
cyoC
b0431: cyoB - cytochrome o ubiquinol oxidase subunit I (NCBI), at 447,874 to 449,865
cyoB
b0432: cyoA - cytochrome o ubiquinol oxidase subunit II (NCBI), at 449,887 to 450,834
cyoA
Position (kb) 447
448
449
450 Strain fitness (log2 ratio) -1
0
1
2
3
4
5
6 at 446.880 kb on + strand at 446.880 kb on + strand at 446.932 kb on + strand at 446.944 kb on + strand at 446.981 kb on - strand, within cyoD at 447.018 kb on - strand, within cyoD at 447.093 kb on - strand, within cyoD at 447.093 kb on - strand, within cyoD at 447.105 kb on - strand, within cyoD at 447.161 kb on - strand, within cyoD at 447.167 kb on + strand, within cyoD at 447.175 kb on - strand, within cyoD at 447.201 kb on + strand, within cyoD at 447.223 kb on - strand, within cyoD at 447.285 kb on - strand at 447.303 kb on - strand at 447.347 kb on + strand, within cyoC at 447.351 kb on + strand, within cyoC at 447.373 kb on - strand, within cyoC at 447.407 kb on + strand, within cyoC at 447.418 kb on - strand, within cyoC at 447.445 kb on - strand, within cyoC at 447.492 kb on + strand, within cyoC at 447.492 kb on + strand, within cyoC at 447.521 kb on + strand, within cyoC at 447.570 kb on + strand, within cyoC at 447.580 kb on + strand, within cyoC at 447.679 kb on + strand, within cyoC at 447.956 kb on + strand at 447.964 kb on - strand at 447.964 kb on - strand at 447.989 kb on + strand at 448.002 kb on - strand at 448.005 kb on + strand at 448.092 kb on - strand, within cyoB at 448.092 kb on - strand, within cyoB at 448.134 kb on - strand, within cyoB at 448.134 kb on - strand, within cyoB at 448.238 kb on - strand, within cyoB at 448.364 kb on - strand, within cyoB at 448.364 kb on - strand, within cyoB at 448.388 kb on + strand, within cyoB at 448.388 kb on + strand, within cyoB at 448.388 kb on + strand, within cyoB at 448.391 kb on - strand, within cyoB at 448.391 kb on - strand, within cyoB at 448.396 kb on - strand, within cyoB at 448.396 kb on - strand, within cyoB at 448.396 kb on - strand, within cyoB at 448.434 kb on - strand, within cyoB at 448.443 kb on - strand, within cyoB at 448.443 kb on - strand, within cyoB at 448.455 kb on - strand, within cyoB at 448.457 kb on + strand, within cyoB at 448.466 kb on + strand, within cyoB at 448.549 kb on + strand, within cyoB at 448.557 kb on - strand, within cyoB at 448.641 kb on - strand, within cyoB at 448.678 kb on + strand, within cyoB at 448.722 kb on + strand, within cyoB at 448.722 kb on - strand, within cyoB at 448.730 kb on - strand, within cyoB at 448.750 kb on - strand, within cyoB at 448.844 kb on + strand, within cyoB at 448.844 kb on + strand, within cyoB at 448.846 kb on + strand, within cyoB at 448.854 kb on + strand, within cyoB at 448.854 kb on + strand, within cyoB at 448.854 kb on - strand, within cyoB at 448.880 kb on + strand, within cyoB at 449.038 kb on + strand, within cyoB at 449.041 kb on - strand, within cyoB at 449.053 kb on - strand, within cyoB at 449.056 kb on - strand, within cyoB at 449.098 kb on + strand, within cyoB at 449.098 kb on + strand, within cyoB at 449.135 kb on + strand, within cyoB at 449.169 kb on + strand, within cyoB at 449.169 kb on + strand, within cyoB at 449.202 kb on + strand at 449.210 kb on - strand, within cyoB at 449.210 kb on - strand, within cyoB at 449.278 kb on + strand, within cyoB at 449.299 kb on + strand, within cyoB at 449.299 kb on + strand, within cyoB at 449.323 kb on + strand, within cyoB at 449.330 kb on + strand, within cyoB at 449.438 kb on - strand, within cyoB at 449.580 kb on - strand, within cyoB at 449.631 kb on + strand, within cyoB at 449.681 kb on + strand at 449.686 kb on - strand at 449.686 kb on - strand at 449.690 kb on + strand at 449.711 kb on + strand at 449.729 kb on + strand at 449.756 kb on + strand at 449.773 kb on - strand at 449.773 kb on - strand at 449.776 kb on + strand at 449.783 kb on - strand at 449.785 kb on + strand at 449.785 kb on + strand at 449.817 kb on + strand at 449.830 kb on + strand at 449.848 kb on - strand at 449.882 kb on + strand at 449.882 kb on + strand at 449.892 kb on - strand at 449.892 kb on - strand at 449.893 kb on + strand at 449.893 kb on + strand at 449.893 kb on + strand at 449.908 kb on + strand at 449.908 kb on + strand at 449.908 kb on + strand at 449.908 kb on + strand at 449.916 kb on - strand at 449.975 kb on + strand at 449.976 kb on - strand at 449.977 kb on - strand at 449.977 kb on - strand at 449.983 kb on - strand, within cyoA at 449.983 kb on - strand, within cyoA at 449.983 kb on - strand, within cyoA at 449.987 kb on + strand, within cyoA at 450.027 kb on - strand, within cyoA at 450.047 kb on + strand, within cyoA at 450.112 kb on - strand, within cyoA at 450.178 kb on - strand, within cyoA at 450.201 kb on - strand, within cyoA at 450.221 kb on - strand, within cyoA at 450.221 kb on - strand, within cyoA at 450.344 kb on + strand, within cyoA at 450.344 kb on + strand, within cyoA at 450.353 kb on + strand, within cyoA at 450.359 kb on - strand, within cyoA at 450.367 kb on - strand, within cyoA at 450.367 kb on - strand, within cyoA at 450.397 kb on + strand, within cyoA at 450.423 kb on - strand, within cyoA at 450.432 kb on - strand, within cyoA at 450.452 kb on - strand, within cyoA at 450.452 kb on - strand, within cyoA at 450.463 kb on + strand, within cyoA at 450.497 kb on - strand, within cyoA at 450.514 kb on + strand, within cyoA at 450.522 kb on + strand, within cyoA at 450.546 kb on - strand, within cyoA at 450.553 kb on + strand, within cyoA at 450.562 kb on - strand, within cyoA at 450.562 kb on - strand, within cyoA at 450.575 kb on + strand, within cyoA at 450.581 kb on - strand, within cyoA at 450.597 kb on + strand, within cyoA at 450.601 kb on + strand, within cyoA at 450.601 kb on - strand, within cyoA at 450.673 kb on - strand, within cyoA at 450.687 kb on + strand, within cyoA at 450.702 kb on + strand, within cyoA at 450.707 kb on + strand, within cyoA at 450.710 kb on - strand, within cyoA at 450.715 kb on - strand, within cyoA at 450.718 kb on + strand, within cyoA at 450.725 kb on - strand, within cyoA at 450.730 kb on - strand, within cyoA at 450.730 kb on - strand, within cyoA at 450.753 kb on - strand at 450.763 kb on - strand at 450.769 kb on + strand at 450.769 kb on + strand at 450.774 kb on + strand at 450.777 kb on - strand at 450.779 kb on - strand at 450.779 kb on - strand at 450.782 kb on - strand at 450.782 kb on - strand at 450.792 kb on + strand at 450.863 kb on - strand at 450.863 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.0125 mg/ml remove 446,880 + +4.3 446,880 + +4.1 446,932 + +4.6 446,944 + +4.6 446,981 - cyoD b0429 0.12 +3.6 447,018 - cyoD b0429 0.23 +5.8 447,093 - cyoD b0429 0.46 +3.9 447,093 - cyoD b0429 0.46 +3.4 447,105 - cyoD b0429 0.50 +4.5 447,161 - cyoD b0429 0.67 +4.8 447,167 + cyoD b0429 0.68 +3.3 447,175 - cyoD b0429 0.71 +3.6 447,201 + cyoD b0429 0.79 +3.7 447,223 - cyoD b0429 0.85 +4.1 447,285 - +3.6 447,303 - +3.3 447,347 + cyoC b0430 0.13 +4.5 447,351 + cyoC b0430 0.13 +3.0 447,373 - cyoC b0430 0.17 +4.5 447,407 + cyoC b0430 0.22 +2.9 447,418 - cyoC b0430 0.24 +2.7 447,445 - cyoC b0430 0.28 +3.3 447,492 + cyoC b0430 0.36 +2.5 447,492 + cyoC b0430 0.36 +3.4 447,521 + cyoC b0430 0.41 +3.9 447,570 + cyoC b0430 0.49 +4.2 447,580 + cyoC b0430 0.50 +4.8 447,679 + cyoC b0430 0.67 +4.3 447,956 + +4.6 447,964 - +1.6 447,964 - +0.9 447,989 + +5.3 448,002 - +4.1 448,005 + +4.2 448,092 - cyoB b0431 0.11 +3.2 448,092 - cyoB b0431 0.11 +3.9 448,134 - cyoB b0431 0.13 +3.8 448,134 - cyoB b0431 0.13 +2.0 448,238 - cyoB b0431 0.18 +3.3 448,364 - cyoB b0431 0.25 +3.2 448,364 - cyoB b0431 0.25 +3.2 448,388 + cyoB b0431 0.26 +5.6 448,388 + cyoB b0431 0.26 +4.2 448,388 + cyoB b0431 0.26 +4.3 448,391 - cyoB b0431 0.26 +2.9 448,391 - cyoB b0431 0.26 +3.1 448,396 - cyoB b0431 0.26 +3.4 448,396 - cyoB b0431 0.26 +2.1 448,396 - cyoB b0431 0.26 +2.9 448,434 - cyoB b0431 0.28 +3.3 448,443 - cyoB b0431 0.29 +3.4 448,443 - cyoB b0431 0.29 +2.7 448,455 - cyoB b0431 0.29 +3.8 448,457 + cyoB b0431 0.29 +4.5 448,466 + cyoB b0431 0.30 +5.2 448,549 + cyoB b0431 0.34 +4.3 448,557 - cyoB b0431 0.34 +3.9 448,641 - cyoB b0431 0.39 +3.3 448,678 + cyoB b0431 0.40 +4.0 448,722 + cyoB b0431 0.43 +4.1 448,722 - cyoB b0431 0.43 +3.8 448,730 - cyoB b0431 0.43 +4.1 448,750 - cyoB b0431 0.44 +4.8 448,844 + cyoB b0431 0.49 +4.5 448,844 + cyoB b0431 0.49 +3.6 448,846 + cyoB b0431 0.49 +4.2 448,854 + cyoB b0431 0.49 +3.7 448,854 + cyoB b0431 0.49 +3.2 448,854 - cyoB b0431 0.49 +3.0 448,880 + cyoB b0431 0.51 +4.1 449,038 + cyoB b0431 0.58 +4.0 449,041 - cyoB b0431 0.59 +3.7 449,053 - cyoB b0431 0.59 +3.3 449,056 - cyoB b0431 0.59 +2.1 449,098 + cyoB b0431 0.61 +3.8 449,098 + cyoB b0431 0.61 +4.6 449,135 + cyoB b0431 0.63 +4.6 449,169 + cyoB b0431 0.65 +4.9 449,169 + cyoB b0431 0.65 +3.9 449,202 + +2.9 449,210 - cyoB b0431 0.67 +5.6 449,210 - cyoB b0431 0.67 +4.0 449,278 + cyoB b0431 0.70 +4.0 449,299 + cyoB b0431 0.72 +4.4 449,299 + cyoB b0431 0.72 +3.3 449,323 + cyoB b0431 0.73 +4.1 449,330 + cyoB b0431 0.73 +3.7 449,438 - cyoB b0431 0.79 +4.3 449,580 - cyoB b0431 0.86 +4.0 449,631 + cyoB b0431 0.88 +4.0 449,681 + +4.0 449,686 - +3.1 449,686 - +2.9 449,690 + +4.6 449,711 + +3.9 449,729 + +4.1 449,756 + +4.2 449,773 - +5.2 449,773 - +1.6 449,776 + +5.0 449,783 - +4.6 449,785 + +5.0 449,785 + +4.4 449,817 + +3.2 449,830 + +4.9 449,848 - +4.6 449,882 + +2.8 449,882 + +4.1 449,892 - +2.0 449,892 - +2.2 449,893 + +3.7 449,893 + +2.1 449,893 + +3.7 449,908 + +4.5 449,908 + +4.8 449,908 + +4.5 449,908 + +4.7 449,916 - -0.7 449,975 + +4.2 449,976 - +0.7 449,977 - +1.3 449,977 - +2.0 449,983 - cyoA b0432 0.10 +0.7 449,983 - cyoA b0432 0.10 +0.7 449,983 - cyoA b0432 0.10 +1.5 449,987 + cyoA b0432 0.11 +5.0 450,027 - cyoA b0432 0.15 +4.5 450,047 + cyoA b0432 0.17 +3.6 450,112 - cyoA b0432 0.24 +4.4 450,178 - cyoA b0432 0.31 +3.6 450,201 - cyoA b0432 0.33 +5.0 450,221 - cyoA b0432 0.35 +3.4 450,221 - cyoA b0432 0.35 +3.4 450,344 + cyoA b0432 0.48 +5.0 450,344 + cyoA b0432 0.48 +4.1 450,353 + cyoA b0432 0.49 +4.7 450,359 - cyoA b0432 0.50 +4.2 450,367 - cyoA b0432 0.51 +4.1 450,367 - cyoA b0432 0.51 +4.4 450,397 + cyoA b0432 0.54 +4.5 450,423 - cyoA b0432 0.57 +4.2 450,432 - cyoA b0432 0.57 +4.3 450,452 - cyoA b0432 0.60 +4.3 450,452 - cyoA b0432 0.60 +4.2 450,463 + cyoA b0432 0.61 +4.6 450,497 - cyoA b0432 0.64 +3.6 450,514 + cyoA b0432 0.66 +4.4 450,522 + cyoA b0432 0.67 +5.3 450,546 - cyoA b0432 0.70 +5.6 450,553 + cyoA b0432 0.70 +5.0 450,562 - cyoA b0432 0.71 -0.1 450,562 - cyoA b0432 0.71 +3.1 450,575 + cyoA b0432 0.73 +4.8 450,581 - cyoA b0432 0.73 +4.1 450,597 + cyoA b0432 0.75 +4.1 450,601 + cyoA b0432 0.75 +4.2 450,601 - cyoA b0432 0.75 +3.4 450,673 - cyoA b0432 0.83 +4.1 450,687 + cyoA b0432 0.84 +4.4 450,702 + cyoA b0432 0.86 +3.4 450,707 + cyoA b0432 0.86 +4.2 450,710 - cyoA b0432 0.87 +4.4 450,715 - cyoA b0432 0.87 +4.0 450,718 + cyoA b0432 0.88 +5.3 450,725 - cyoA b0432 0.88 +4.8 450,730 - cyoA b0432 0.89 +4.4 450,730 - cyoA b0432 0.89 +5.2 450,753 - +4.4 450,763 - +5.2 450,769 + +4.1 450,769 + +4.3 450,774 + +4.8 450,777 - +4.4 450,779 - +5.1 450,779 - +3.1 450,782 - +4.8 450,782 - +5.8 450,792 + +4.2 450,863 - +5.6 450,863 - +2.3
Or see this region's nucleotide sequence