Experiment: Spectinomycin 0.0125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt proS and yaeB are separated by 111 nucleotides yaeB and rcsF overlap by 4 nucleotides rcsF and metQ are separated by 117 nucleotides
b0194: proS - prolyl-tRNA synthetase (NCBI), at 217,057 to 218,775
proS
b0195: yaeB - hypothetical protein (NCBI), at 218,887 to 219,594
yaeB
b0196: rcsF - predicted outer membrane protein, signal (NCBI), at 219,591 to 219,995
rcsF
b0197: metQ - DL-methionine transporter subunit (NCBI), at 220,113 to 220,928
metQ
Position (kb) 218
219
220 Strain fitness (log2 ratio) -2
-1
0
1
2 at 218.821 kb on - strand at 218.871 kb on + strand at 218.910 kb on + strand at 218.910 kb on + strand at 218.965 kb on + strand, within yaeB at 218.974 kb on - strand, within yaeB at 219.027 kb on - strand, within yaeB at 219.071 kb on - strand, within yaeB at 219.200 kb on + strand, within yaeB at 219.240 kb on + strand, within yaeB at 219.240 kb on + strand, within yaeB at 219.261 kb on + strand, within yaeB at 219.312 kb on + strand, within yaeB at 219.446 kb on - strand, within yaeB at 219.465 kb on + strand, within yaeB at 219.466 kb on + strand, within yaeB at 219.489 kb on - strand, within yaeB at 219.513 kb on + strand, within yaeB at 219.548 kb on - strand at 219.598 kb on + strand at 219.598 kb on + strand at 219.643 kb on - strand, within rcsF at 219.678 kb on + strand, within rcsF at 219.680 kb on + strand, within rcsF at 219.680 kb on + strand, within rcsF at 219.680 kb on + strand, within rcsF at 219.720 kb on - strand, within rcsF at 219.720 kb on - strand, within rcsF at 219.756 kb on + strand, within rcsF at 219.764 kb on - strand, within rcsF at 219.764 kb on - strand, within rcsF at 219.764 kb on - strand, within rcsF at 219.842 kb on - strand, within rcsF at 219.902 kb on + strand, within rcsF at 219.944 kb on - strand, within rcsF at 219.968 kb on + strand at 219.976 kb on - strand at 220.041 kb on - strand at 220.041 kb on - strand at 220.041 kb on - strand at 220.127 kb on - strand at 220.127 kb on - strand at 220.249 kb on - strand, within metQ at 220.321 kb on + strand, within metQ at 220.321 kb on + strand, within metQ at 220.323 kb on + strand, within metQ at 220.361 kb on - strand, within metQ at 220.386 kb on - strand, within metQ at 220.386 kb on - strand, within metQ at 220.405 kb on + strand, within metQ at 220.405 kb on + strand, within metQ at 220.432 kb on + strand, within metQ at 220.432 kb on + strand, within metQ at 220.504 kb on + strand, within metQ at 220.532 kb on + strand, within metQ at 220.543 kb on + strand, within metQ
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.0125 mg/ml remove 218,821 - -0.6 218,871 + +0.3 218,910 + +0.3 218,910 + -1.0 218,965 + yaeB b0195 0.11 +0.1 218,974 - yaeB b0195 0.12 +0.7 219,027 - yaeB b0195 0.20 -0.5 219,071 - yaeB b0195 0.26 -0.9 219,200 + yaeB b0195 0.44 -0.3 219,240 + yaeB b0195 0.50 -2.4 219,240 + yaeB b0195 0.50 -0.7 219,261 + yaeB b0195 0.53 -0.2 219,312 + yaeB b0195 0.60 -0.5 219,446 - yaeB b0195 0.79 -1.3 219,465 + yaeB b0195 0.82 -1.2 219,466 + yaeB b0195 0.82 +0.1 219,489 - yaeB b0195 0.85 +0.3 219,513 + yaeB b0195 0.88 -0.8 219,548 - -0.4 219,598 + -0.4 219,598 + -0.5 219,643 - rcsF b0196 0.13 -0.5 219,678 + rcsF b0196 0.21 +1.5 219,680 + rcsF b0196 0.22 -0.5 219,680 + rcsF b0196 0.22 -0.4 219,680 + rcsF b0196 0.22 -0.8 219,720 - rcsF b0196 0.32 -0.3 219,720 - rcsF b0196 0.32 +0.1 219,756 + rcsF b0196 0.41 -0.3 219,764 - rcsF b0196 0.43 +0.2 219,764 - rcsF b0196 0.43 -0.5 219,764 - rcsF b0196 0.43 -0.4 219,842 - rcsF b0196 0.62 -1.1 219,902 + rcsF b0196 0.77 -0.8 219,944 - rcsF b0196 0.87 +1.1 219,968 + +1.3 219,976 - +0.1 220,041 - -1.7 220,041 - +0.5 220,041 - -0.5 220,127 - +2.6 220,127 - -0.5 220,249 - metQ b0197 0.17 -0.3 220,321 + metQ b0197 0.25 +1.7 220,321 + metQ b0197 0.25 +0.3 220,323 + metQ b0197 0.26 +0.3 220,361 - metQ b0197 0.30 -0.0 220,386 - metQ b0197 0.33 -1.8 220,386 - metQ b0197 0.33 +0.8 220,405 + metQ b0197 0.36 -0.4 220,405 + metQ b0197 0.36 -0.9 220,432 + metQ b0197 0.39 +1.4 220,432 + metQ b0197 0.39 +0.4 220,504 + metQ b0197 0.48 +1.1 220,532 + metQ b0197 0.51 -0.9 220,543 + metQ b0197 0.53 +0.2
Or see this region's nucleotide sequence