Strain Fitness in Escherichia coli BW25113 around b0152

Experiment: Spectinomycin 0.0125 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntfhuA and fhuC are separated by 50 nucleotidesfhuC and fhuD overlap by 1 nucleotidesfhuD and fhuB overlap by 4 nucleotides b0150: fhuA - ferrichrome outer membrane transporter (NCBI), at 167,484 to 169,727 fhuA b0151: fhuC - iron-hydroxamate transporter subunit (NCBI), at 169,778 to 170,575 fhuC b0152: fhuD - iron-hydroxamate transporter subunit (NCBI), at 170,575 to 171,465 fhuD b0153: fhuB - fused iron-hydroxamate transporter subunits of ABC superfamily: membrane components (NCBI), at 171,462 to 173,444 fhuB Position (kb) 170 171 172Strain fitness (log2 ratio) -2 -1 0 1 2 3 4 5at 169.594 kb on - strandat 169.620 kb on + strandat 169.629 kb on - strandat 169.635 kb on - strandat 169.635 kb on - strandat 169.639 kb on - strandat 169.684 kb on - strandat 169.827 kb on - strandat 169.926 kb on + strand, within fhuCat 169.926 kb on + strand, within fhuCat 169.926 kb on + strand, within fhuCat 169.929 kb on - strand, within fhuCat 169.965 kb on + strand, within fhuCat 169.966 kb on + strand, within fhuCat 169.966 kb on + strand, within fhuCat 169.989 kb on + strand, within fhuCat 170.210 kb on + strand, within fhuCat 170.210 kb on + strand, within fhuCat 170.250 kb on + strand, within fhuCat 170.250 kb on - strand, within fhuCat 170.255 kb on + strand, within fhuCat 170.257 kb on + strand, within fhuCat 170.390 kb on - strand, within fhuCat 170.390 kb on - strand, within fhuCat 170.395 kb on - strand, within fhuCat 170.654 kb on - strandat 170.671 kb on + strand, within fhuDat 170.671 kb on + strand, within fhuDat 170.689 kb on - strand, within fhuDat 170.739 kb on + strand, within fhuDat 170.739 kb on + strand, within fhuDat 170.755 kb on - strand, within fhuDat 170.755 kb on - strand, within fhuDat 170.775 kb on + strand, within fhuDat 170.831 kb on + strand, within fhuDat 170.869 kb on + strand, within fhuDat 170.869 kb on + strand, within fhuDat 170.895 kb on - strand, within fhuDat 170.895 kb on - strand, within fhuDat 170.972 kb on - strand, within fhuDat 171.065 kb on + strand, within fhuDat 171.199 kb on + strand, within fhuDat 171.199 kb on + strand, within fhuDat 171.219 kb on + strand, within fhuDat 171.225 kb on + strand, within fhuDat 171.225 kb on + strand, within fhuDat 171.228 kb on + strand, within fhuDat 171.228 kb on + strand, within fhuDat 171.238 kb on - strand, within fhuDat 171.238 kb on - strand, within fhuDat 171.307 kb on - strand, within fhuDat 171.336 kb on + strand, within fhuDat 171.336 kb on + strand, within fhuDat 171.423 kb on + strandat 171.423 kb on - strandat 171.423 kb on - strandat 171.506 kb on + strandat 171.514 kb on - strandat 171.533 kb on - strandat 171.678 kb on + strand, within fhuBat 171.678 kb on + strand, within fhuBat 171.680 kb on + strand, within fhuBat 171.680 kb on + strand, within fhuBat 171.680 kb on - strand, within fhuBat 171.680 kb on - strand, within fhuBat 171.695 kb on + strand, within fhuBat 171.742 kb on + strand, within fhuBat 171.794 kb on + strand, within fhuBat 171.867 kb on + strand, within fhuBat 171.910 kb on + strand, within fhuBat 171.910 kb on + strand, within fhuBat 172.063 kb on + strand, within fhuBat 172.088 kb on - strand, within fhuBat 172.202 kb on + strand, within fhuBat 172.218 kb on - strand, within fhuBat 172.222 kb on - strand, within fhuBat 172.261 kb on + strand, within fhuBat 172.281 kb on + strand, within fhuBat 172.281 kb on + strand, within fhuBat 172.289 kb on + strand, within fhuBat 172.290 kb on - strand, within fhuBat 172.293 kb on + strand, within fhuBat 172.293 kb on + strand, within fhuBat 172.314 kb on + strand, within fhuBat 172.369 kb on - strand, within fhuB

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Per-strain Table

Position Strand Gene LocusTag Fraction Spectinomycin 0.0125 mg/ml
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169,594 - -0.5
169,620 + -0.7
169,629 - -0.1
169,635 - -0.2
169,635 - +1.7
169,639 - +0.6
169,684 - +0.9
169,827 - +0.6
169,926 + fhuC b0151 0.19 +0.3
169,926 + fhuC b0151 0.19 +1.1
169,926 + fhuC b0151 0.19 -0.7
169,929 - fhuC b0151 0.19 -0.2
169,965 + fhuC b0151 0.23 +0.4
169,966 + fhuC b0151 0.24 +0.1
169,966 + fhuC b0151 0.24 +0.1
169,989 + fhuC b0151 0.26 +0.3
170,210 + fhuC b0151 0.54 -0.9
170,210 + fhuC b0151 0.54 +1.9
170,250 + fhuC b0151 0.59 -1.3
170,250 - fhuC b0151 0.59 -0.3
170,255 + fhuC b0151 0.60 +0.3
170,257 + fhuC b0151 0.60 -2.7
170,390 - fhuC b0151 0.77 -0.7
170,390 - fhuC b0151 0.77 -1.2
170,395 - fhuC b0151 0.77 -1.4
170,654 - +0.8
170,671 + fhuD b0152 0.11 +0.0
170,671 + fhuD b0152 0.11 +0.7
170,689 - fhuD b0152 0.13 -0.4
170,739 + fhuD b0152 0.18 -0.5
170,739 + fhuD b0152 0.18 +1.1
170,755 - fhuD b0152 0.20 +2.1
170,755 - fhuD b0152 0.20 +0.3
170,775 + fhuD b0152 0.22 -0.9
170,831 + fhuD b0152 0.29 -0.9
170,869 + fhuD b0152 0.33 -0.1
170,869 + fhuD b0152 0.33 +0.6
170,895 - fhuD b0152 0.36 -1.7
170,895 - fhuD b0152 0.36 -0.3
170,972 - fhuD b0152 0.45 -0.9
171,065 + fhuD b0152 0.55 +0.8
171,199 + fhuD b0152 0.70 +0.5
171,199 + fhuD b0152 0.70 +1.3
171,219 + fhuD b0152 0.72 -1.2
171,225 + fhuD b0152 0.73 +0.8
171,225 + fhuD b0152 0.73 +0.1
171,228 + fhuD b0152 0.73 +0.1
171,228 + fhuD b0152 0.73 +0.1
171,238 - fhuD b0152 0.74 +4.8
171,238 - fhuD b0152 0.74 +5.4
171,307 - fhuD b0152 0.82 -1.1
171,336 + fhuD b0152 0.85 +1.1
171,336 + fhuD b0152 0.85 +0.3
171,423 + -0.2
171,423 - -0.2
171,423 - +1.1
171,506 + -0.1
171,514 - -0.2
171,533 - -0.3
171,678 + fhuB b0153 0.11 +0.9
171,678 + fhuB b0153 0.11 -0.7
171,680 + fhuB b0153 0.11 +1.7
171,680 + fhuB b0153 0.11 +0.3
171,680 - fhuB b0153 0.11 +0.2
171,680 - fhuB b0153 0.11 +1.5
171,695 + fhuB b0153 0.12 -0.2
171,742 + fhuB b0153 0.14 +0.2
171,794 + fhuB b0153 0.17 -0.4
171,867 + fhuB b0153 0.20 -2.1
171,910 + fhuB b0153 0.23 -1.2
171,910 + fhuB b0153 0.23 -1.7
172,063 + fhuB b0153 0.30 +0.3
172,088 - fhuB b0153 0.32 +1.1
172,202 + fhuB b0153 0.37 +0.1
172,218 - fhuB b0153 0.38 +0.8
172,222 - fhuB b0153 0.38 -1.5
172,261 + fhuB b0153 0.40 +3.1
172,281 + fhuB b0153 0.41 -0.1
172,281 + fhuB b0153 0.41 +0.3
172,289 + fhuB b0153 0.42 -0.5
172,290 - fhuB b0153 0.42 +0.6
172,293 + fhuB b0153 0.42 +1.1
172,293 + fhuB b0153 0.42 +0.5
172,314 + fhuB b0153 0.43 +1.1
172,369 - fhuB b0153 0.46 -0.5

Or see this region's nucleotide sequence