Experiment: 150_an_nonmag_2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS12305 and AMB_RS12310 overlap by 4 nucleotides AMB_RS12310 and AMB_RS12315 are separated by 5 nucleotides AMB_RS12315 and AMB_RS12320 are separated by 18 nucleotides
AMB_RS12305: AMB_RS12305 - DNA polymerase III subunit delta', at 2,625,545 to 2,626,636
_RS12305
AMB_RS12310: AMB_RS12310 - dTMP kinase, at 2,626,633 to 2,627,271
_RS12310
AMB_RS12315: AMB_RS12315 - D-alanyl-D-alanine carboxypeptidase, at 2,627,277 to 2,628,440
_RS12315
AMB_RS12320: AMB_RS12320 - septal ring lytic transglycosylase RlpA family protein, at 2,628,459 to 2,629,433
_RS12320
Position (kb)
2627
2628
2629 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2627.459 kb on - strand, within AMB_RS12315 at 2627.459 kb on - strand, within AMB_RS12315 at 2627.459 kb on - strand, within AMB_RS12315 at 2627.606 kb on - strand, within AMB_RS12315 at 2627.606 kb on - strand, within AMB_RS12315 at 2627.606 kb on - strand, within AMB_RS12315 at 2627.606 kb on - strand, within AMB_RS12315 at 2628.320 kb on - strand, within AMB_RS12315 at 2628.461 kb on - strand at 2628.677 kb on - strand, within AMB_RS12320 at 2628.677 kb on - strand, within AMB_RS12320 at 2629.286 kb on - strand, within AMB_RS12320 at 2629.286 kb on - strand, within AMB_RS12320
Per-strain Table
Position Strand Gene LocusTag Fraction 150_an_nonmag_2 remove 2,627,459 - AMB_RS12315 0.16 +0.1 2,627,459 - AMB_RS12315 0.16 -3.2 2,627,459 - AMB_RS12315 0.16 -1.0 2,627,606 - AMB_RS12315 0.28 +1.1 2,627,606 - AMB_RS12315 0.28 -4.3 2,627,606 - AMB_RS12315 0.28 -0.2 2,627,606 - AMB_RS12315 0.28 -0.5 2,628,320 - AMB_RS12315 0.90 +0.4 2,628,461 - -0.0 2,628,677 - AMB_RS12320 0.22 +1.6 2,628,677 - AMB_RS12320 0.22 +0.2 2,629,286 - AMB_RS12320 0.85 +0.9 2,629,286 - AMB_RS12320 0.85 -0.7
Or see this region's nucleotide sequence