Experiment: 150_an_nonmag_2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS11140 and AMB_RS11145 overlap by 4 nucleotides AMB_RS11145 and AMB_RS11150 are separated by 44 nucleotides AMB_RS11150 and AMB_RS11155 are separated by 203 nucleotides
AMB_RS11140: AMB_RS11140 - 5-(carboxyamino)imidazole ribonucleotide synthase, at 2,397,158 to 2,398,267
_RS11140
AMB_RS11145: AMB_RS11145 - 5-(carboxyamino)imidazole ribonucleotide mutase, at 2,398,264 to 2,398,785
_RS11145
AMB_RS11150: AMB_RS11150 - GGDEF domain-containing protein, at 2,398,830 to 2,399,675
_RS11150
AMB_RS11155: AMB_RS11155 - DNA-binding response regulator, at 2,399,879 to 2,400,547
_RS11155
Position (kb)
2398
2399
2400 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2398.806 kb on - strand at 2398.896 kb on + strand at 2398.896 kb on + strand at 2398.897 kb on - strand at 2399.212 kb on - strand, within AMB_RS11150 at 2399.553 kb on + strand, within AMB_RS11150 at 2399.554 kb on - strand, within AMB_RS11150 at 2399.554 kb on - strand, within AMB_RS11150 at 2399.596 kb on - strand at 2399.681 kb on + strand at 2399.965 kb on - strand, within AMB_RS11155 at 2400.177 kb on + strand, within AMB_RS11155 at 2400.178 kb on - strand, within AMB_RS11155 at 2400.178 kb on - strand, within AMB_RS11155 at 2400.178 kb on - strand, within AMB_RS11155 at 2400.297 kb on + strand, within AMB_RS11155 at 2400.669 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 150_an_nonmag_2 remove 2,398,806 - -1.5 2,398,896 + +1.1 2,398,896 + +0.1 2,398,897 - -3.0 2,399,212 - AMB_RS11150 0.45 -0.7 2,399,553 + AMB_RS11150 0.85 -1.3 2,399,554 - AMB_RS11150 0.86 +1.0 2,399,554 - AMB_RS11150 0.86 -0.3 2,399,596 - -2.2 2,399,681 + -2.5 2,399,965 - AMB_RS11155 0.13 -0.8 2,400,177 + AMB_RS11155 0.45 -1.5 2,400,178 - AMB_RS11155 0.45 -0.8 2,400,178 - AMB_RS11155 0.45 -2.5 2,400,178 - AMB_RS11155 0.45 -3.0 2,400,297 + AMB_RS11155 0.62 -0.8 2,400,669 - -0.4
Or see this region's nucleotide sequence