Experiment: 150_an_nonmag_2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS08295 and AMB_RS08300 overlap by 4 nucleotides AMB_RS08300 and AMB_RS08305 are separated by 20 nucleotides AMB_RS08305 and AMB_RS08310 overlap by 4 nucleotides AMB_RS08310 and AMB_RS08315 overlap by 1 nucleotides
AMB_RS08295: AMB_RS08295 - hydrogenase formation protein HypD, at 1,778,045 to 1,779,184
_RS08295
AMB_RS08300: AMB_RS08300 - HypC/HybG/HupF family hydrogenase formation chaperone, at 1,779,181 to 1,779,417
_RS08300
AMB_RS08305: AMB_RS08305 - hypothetical protein, at 1,779,438 to 1,780,514
_RS08305
AMB_RS08310: AMB_RS08310 - hydrogenase accessory protein HypB, at 1,780,511 to 1,781,449
_RS08310
AMB_RS08315: AMB_RS08315 - hydrogenase maturation nickel metallochaperone HypA, at 1,781,449 to 1,781,793
_RS08315
Position (kb)
1779
1780
1781 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 1778.526 kb on + strand, within AMB_RS08295 at 1778.526 kb on + strand, within AMB_RS08295 at 1778.527 kb on - strand, within AMB_RS08295 at 1778.527 kb on - strand, within AMB_RS08295 at 1778.541 kb on + strand, within AMB_RS08295 at 1778.541 kb on + strand, within AMB_RS08295 at 1778.541 kb on + strand, within AMB_RS08295 at 1778.541 kb on + strand, within AMB_RS08295 at 1778.542 kb on - strand, within AMB_RS08295 at 1778.542 kb on - strand, within AMB_RS08295 at 1778.542 kb on - strand, within AMB_RS08295 at 1778.542 kb on - strand, within AMB_RS08295 at 1778.547 kb on + strand, within AMB_RS08295 at 1778.547 kb on + strand, within AMB_RS08295 at 1778.829 kb on + strand, within AMB_RS08295 at 1778.829 kb on + strand, within AMB_RS08295 at 1778.830 kb on - strand, within AMB_RS08295 at 1778.830 kb on - strand, within AMB_RS08295 at 1778.913 kb on + strand, within AMB_RS08295 at 1778.914 kb on - strand, within AMB_RS08295 at 1779.096 kb on + strand at 1779.096 kb on + strand at 1779.097 kb on - strand at 1779.097 kb on - strand at 1779.097 kb on - strand at 1779.097 kb on - strand at 1779.097 kb on - strand at 1779.177 kb on + strand at 1779.178 kb on - strand at 1779.293 kb on + strand, within AMB_RS08300 at 1779.293 kb on + strand, within AMB_RS08300 at 1779.294 kb on - strand, within AMB_RS08300 at 1779.294 kb on - strand, within AMB_RS08300 at 1779.435 kb on + strand at 1779.436 kb on - strand at 1779.436 kb on - strand at 1779.436 kb on - strand at 1779.436 kb on - strand at 1779.598 kb on + strand, within AMB_RS08305 at 1779.598 kb on + strand, within AMB_RS08305 at 1779.599 kb on - strand, within AMB_RS08305 at 1779.599 kb on - strand, within AMB_RS08305 at 1779.599 kb on - strand, within AMB_RS08305 at 1779.599 kb on - strand, within AMB_RS08305 at 1779.599 kb on - strand, within AMB_RS08305 at 1779.599 kb on - strand, within AMB_RS08305 at 1780.265 kb on + strand, within AMB_RS08305 at 1780.265 kb on + strand, within AMB_RS08305 at 1780.266 kb on - strand, within AMB_RS08305 at 1780.266 kb on - strand, within AMB_RS08305 at 1780.516 kb on + strand at 1780.517 kb on - strand at 1780.830 kb on + strand, within AMB_RS08310 at 1780.830 kb on + strand, within AMB_RS08310 at 1780.830 kb on + strand, within AMB_RS08310 at 1780.831 kb on - strand, within AMB_RS08310 at 1780.831 kb on - strand, within AMB_RS08310 at 1780.831 kb on - strand, within AMB_RS08310 at 1780.831 kb on - strand, within AMB_RS08310 at 1780.939 kb on + strand, within AMB_RS08310 at 1780.939 kb on + strand, within AMB_RS08310 at 1780.939 kb on + strand, within AMB_RS08310 at 1780.940 kb on - strand, within AMB_RS08310 at 1781.274 kb on + strand, within AMB_RS08310
Per-strain Table
Position Strand Gene LocusTag Fraction 150_an_nonmag_2 remove 1,778,526 + AMB_RS08295 0.42 -0.0 1,778,526 + AMB_RS08295 0.42 -1.4 1,778,527 - AMB_RS08295 0.42 -0.2 1,778,527 - AMB_RS08295 0.42 -0.4 1,778,541 + AMB_RS08295 0.44 -1.9 1,778,541 + AMB_RS08295 0.44 +1.6 1,778,541 + AMB_RS08295 0.44 -3.6 1,778,541 + AMB_RS08295 0.44 -0.2 1,778,542 - AMB_RS08295 0.44 -0.2 1,778,542 - AMB_RS08295 0.44 -1.9 1,778,542 - AMB_RS08295 0.44 +0.3 1,778,542 - AMB_RS08295 0.44 -0.5 1,778,547 + AMB_RS08295 0.44 +4.7 1,778,547 + AMB_RS08295 0.44 -0.4 1,778,829 + AMB_RS08295 0.69 -1.0 1,778,829 + AMB_RS08295 0.69 -1.4 1,778,830 - AMB_RS08295 0.69 -1.9 1,778,830 - AMB_RS08295 0.69 +0.8 1,778,913 + AMB_RS08295 0.76 -0.5 1,778,914 - AMB_RS08295 0.76 -1.6 1,779,096 + -1.7 1,779,096 + +0.4 1,779,097 - -0.9 1,779,097 - -0.7 1,779,097 - -1.0 1,779,097 - -3.6 1,779,097 - -1.2 1,779,177 + +0.8 1,779,178 - +0.9 1,779,293 + AMB_RS08300 0.47 -1.9 1,779,293 + AMB_RS08300 0.47 -0.4 1,779,294 - AMB_RS08300 0.48 -0.4 1,779,294 - AMB_RS08300 0.48 -0.0 1,779,435 + -0.4 1,779,436 - -2.6 1,779,436 - -0.6 1,779,436 - -0.7 1,779,436 - -1.4 1,779,598 + AMB_RS08305 0.15 -1.9 1,779,598 + AMB_RS08305 0.15 +2.2 1,779,599 - AMB_RS08305 0.15 -0.5 1,779,599 - AMB_RS08305 0.15 -1.9 1,779,599 - AMB_RS08305 0.15 -1.0 1,779,599 - AMB_RS08305 0.15 -1.3 1,779,599 - AMB_RS08305 0.15 -0.4 1,779,599 - AMB_RS08305 0.15 -0.4 1,780,265 + AMB_RS08305 0.77 -0.0 1,780,265 + AMB_RS08305 0.77 +0.2 1,780,266 - AMB_RS08305 0.77 +0.4 1,780,266 - AMB_RS08305 0.77 +0.4 1,780,516 + -0.7 1,780,517 - -1.0 1,780,830 + AMB_RS08310 0.34 -1.4 1,780,830 + AMB_RS08310 0.34 -0.5 1,780,830 + AMB_RS08310 0.34 +0.3 1,780,831 - AMB_RS08310 0.34 -0.3 1,780,831 - AMB_RS08310 0.34 +0.6 1,780,831 - AMB_RS08310 0.34 -1.9 1,780,831 - AMB_RS08310 0.34 -2.7 1,780,939 + AMB_RS08310 0.46 -3.4 1,780,939 + AMB_RS08310 0.46 -0.1 1,780,939 + AMB_RS08310 0.46 -1.0 1,780,940 - AMB_RS08310 0.46 +0.2 1,781,274 + AMB_RS08310 0.81 -0.9
Or see this region's nucleotide sequence