Experiment: 150_an_nonmag_2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS04510 and AMB_RS04515 are separated by 26 nucleotides AMB_RS04515 and AMB_RS04520 are separated by 99 nucleotides AMB_RS04520 and AMB_RS04525 are separated by 88 nucleotides
AMB_RS04510: AMB_RS04510 - ferrous iron transporter, at 945,069 to 945,941
_RS04510
AMB_RS04515: AMB_RS04515 - pyridoxine/pyridoxamine 5'-phosphate oxidase, at 945,968 to 946,555
_RS04515
AMB_RS04520: AMB_RS04520 - glycine zipper 2TM domain-containing protein, at 946,655 to 947,128
_RS04520
AMB_RS04525: AMB_RS04525 - molecular chaperone DnaJ, at 947,217 to 948,137
_RS04525
Position (kb) 946
947
948 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 945.925 kb on + strand at 946.612 kb on - strand at 946.612 kb on - strand at 946.617 kb on - strand at 946.632 kb on + strand at 946.847 kb on + strand, within AMB_RS04520 at 946.847 kb on + strand, within AMB_RS04520 at 946.847 kb on + strand, within AMB_RS04520 at 946.847 kb on + strand, within AMB_RS04520 at 946.847 kb on + strand, within AMB_RS04520 at 947.036 kb on + strand, within AMB_RS04520 at 947.229 kb on + strand at 947.229 kb on + strand at 947.229 kb on + strand at 947.229 kb on + strand at 947.229 kb on + strand at 947.229 kb on + strand at 947.230 kb on - strand at 947.230 kb on - strand at 947.230 kb on - strand at 947.284 kb on - strand at 947.284 kb on - strand at 947.370 kb on + strand, within AMB_RS04525 at 947.370 kb on + strand, within AMB_RS04525 at 948.105 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 150_an_nonmag_2 remove 945,925 + -1.6 946,612 - -0.9 946,612 - -3.3 946,617 - -0.3 946,632 + -1.0 946,847 + AMB_RS04520 0.41 -1.6 946,847 + AMB_RS04520 0.41 -0.7 946,847 + AMB_RS04520 0.41 -1.0 946,847 + AMB_RS04520 0.41 -1.7 946,847 + AMB_RS04520 0.41 +0.2 947,036 + AMB_RS04520 0.80 -0.8 947,229 + +0.1 947,229 + +0.6 947,229 + -0.2 947,229 + -1.0 947,229 + -2.4 947,229 + -1.0 947,230 - -1.0 947,230 - -0.4 947,230 - -0.5 947,284 - +1.0 947,284 - -0.0 947,370 + AMB_RS04525 0.17 -1.4 947,370 + AMB_RS04525 0.17 -3.0 948,105 + -0.7
Or see this region's nucleotide sequence