Experiment: 150_an_nonmag_2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS02420 and AMB_RS02425 overlap by 4 nucleotides AMB_RS02425 and AMB_RS02430 are separated by 3 nucleotides AMB_RS02430 and AMB_RS02435 overlap by 4 nucleotides AMB_RS02435 and AMB_RS23135 are separated by 2 nucleotides AMB_RS23135 and AMB_RS02445 are separated by 1 nucleotides
AMB_RS02420: AMB_RS02420 - hypothetical protein, at 503,373 to 503,768
_RS02420
AMB_RS02425: AMB_RS02425 - hypothetical protein, at 503,765 to 504,091
_RS02425
AMB_RS02430: AMB_RS02430 - DUF3486 domain-containing protein, at 504,095 to 504,712
_RS02430
AMB_RS02435: AMB_RS02435 - hypothetical protein, at 504,709 to 505,185
_RS02435
AMB_RS23135: AMB_RS23135 - DUF2163 domain-containing protein, at 505,188 to 506,078
_RS23135
AMB_RS02445: AMB_RS02445 - hydrolase, at 506,080 to 506,532
_RS02445
Position (kb) 504
505
506 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 503.782 kb on + strand at 503.850 kb on - strand, within AMB_RS02425 at 503.850 kb on - strand, within AMB_RS02425 at 503.850 kb on - strand, within AMB_RS02425 at 503.850 kb on - strand, within AMB_RS02425 at 503.929 kb on + strand, within AMB_RS02425 at 503.929 kb on + strand, within AMB_RS02425 at 503.929 kb on + strand, within AMB_RS02425 at 503.930 kb on - strand, within AMB_RS02425 at 503.930 kb on - strand, within AMB_RS02425 at 503.930 kb on - strand, within AMB_RS02425 at 503.930 kb on - strand, within AMB_RS02425 at 503.930 kb on - strand, within AMB_RS02425 at 504.089 kb on + strand at 504.173 kb on + strand, within AMB_RS02430 at 504.174 kb on - strand, within AMB_RS02430 at 504.174 kb on - strand, within AMB_RS02430 at 504.174 kb on - strand, within AMB_RS02430 at 504.174 kb on - strand, within AMB_RS02430 at 504.174 kb on - strand, within AMB_RS02430 at 504.433 kb on + strand, within AMB_RS02430 at 504.692 kb on + strand at 504.692 kb on + strand at 504.949 kb on + strand, within AMB_RS02435 at 504.949 kb on + strand, within AMB_RS02435 at 504.950 kb on - strand, within AMB_RS02435 at 504.950 kb on - strand, within AMB_RS02435 at 504.980 kb on - strand, within AMB_RS02435 at 504.980 kb on - strand, within AMB_RS02435 at 504.980 kb on - strand, within AMB_RS02435 at 504.980 kb on - strand, within AMB_RS02435 at 504.980 kb on - strand, within AMB_RS02435 at 504.980 kb on - strand, within AMB_RS02435 at 505.015 kb on + strand, within AMB_RS02435 at 505.016 kb on - strand, within AMB_RS02435 at 505.016 kb on - strand, within AMB_RS02435 at 505.016 kb on - strand, within AMB_RS02435 at 505.016 kb on - strand, within AMB_RS02435 at 505.016 kb on - strand, within AMB_RS02435 at 505.084 kb on + strand, within AMB_RS02435 at 505.084 kb on + strand, within AMB_RS02435 at 505.085 kb on - strand, within AMB_RS02435 at 505.085 kb on - strand, within AMB_RS02435 at 505.087 kb on + strand, within AMB_RS02435 at 505.087 kb on + strand, within AMB_RS02435 at 505.087 kb on + strand, within AMB_RS02435 at 505.088 kb on - strand, within AMB_RS02435 at 505.088 kb on - strand, within AMB_RS02435 at 505.088 kb on - strand, within AMB_RS02435 at 505.183 kb on + strand at 505.183 kb on + strand at 505.183 kb on + strand at 505.184 kb on - strand at 505.184 kb on - strand at 505.184 kb on - strand at 505.254 kb on + strand at 505.254 kb on + strand at 505.291 kb on - strand, within AMB_RS23135 at 505.359 kb on + strand, within AMB_RS23135 at 505.530 kb on + strand, within AMB_RS23135 at 505.530 kb on + strand, within AMB_RS23135 at 505.530 kb on + strand, within AMB_RS23135 at 505.530 kb on + strand, within AMB_RS23135 at 505.530 kb on + strand, within AMB_RS23135 at 505.531 kb on - strand, within AMB_RS23135 at 505.531 kb on - strand, within AMB_RS23135 at 505.531 kb on - strand, within AMB_RS23135 at 505.696 kb on - strand, within AMB_RS23135 at 506.034 kb on + strand at 506.035 kb on - strand at 506.143 kb on + strand, within AMB_RS02445 at 506.143 kb on + strand, within AMB_RS02445 at 506.143 kb on + strand, within AMB_RS02445 at 506.144 kb on - strand, within AMB_RS02445
Per-strain Table
Position Strand Gene LocusTag Fraction 150_an_nonmag_2 remove 503,782 + -0.8 503,850 - AMB_RS02425 0.26 -0.2 503,850 - AMB_RS02425 0.26 -3.3 503,850 - AMB_RS02425 0.26 -0.7 503,850 - AMB_RS02425 0.26 -2.1 503,929 + AMB_RS02425 0.50 -1.1 503,929 + AMB_RS02425 0.50 +1.5 503,929 + AMB_RS02425 0.50 +0.2 503,930 - AMB_RS02425 0.50 -1.4 503,930 - AMB_RS02425 0.50 -1.0 503,930 - AMB_RS02425 0.50 -1.2 503,930 - AMB_RS02425 0.50 -1.8 503,930 - AMB_RS02425 0.50 -0.4 504,089 + +0.2 504,173 + AMB_RS02430 0.13 -1.3 504,174 - AMB_RS02430 0.13 +0.1 504,174 - AMB_RS02430 0.13 +0.8 504,174 - AMB_RS02430 0.13 -0.7 504,174 - AMB_RS02430 0.13 -1.0 504,174 - AMB_RS02430 0.13 -0.4 504,433 + AMB_RS02430 0.55 +0.2 504,692 + -1.8 504,692 + -0.6 504,949 + AMB_RS02435 0.50 +0.8 504,949 + AMB_RS02435 0.50 +1.1 504,950 - AMB_RS02435 0.51 -0.1 504,950 - AMB_RS02435 0.51 -2.7 504,980 - AMB_RS02435 0.57 +0.2 504,980 - AMB_RS02435 0.57 -2.9 504,980 - AMB_RS02435 0.57 -1.0 504,980 - AMB_RS02435 0.57 -0.6 504,980 - AMB_RS02435 0.57 -0.2 504,980 - AMB_RS02435 0.57 -1.0 505,015 + AMB_RS02435 0.64 -0.8 505,016 - AMB_RS02435 0.64 -0.5 505,016 - AMB_RS02435 0.64 -1.0 505,016 - AMB_RS02435 0.64 -3.6 505,016 - AMB_RS02435 0.64 -2.1 505,016 - AMB_RS02435 0.64 -3.7 505,084 + AMB_RS02435 0.79 -1.8 505,084 + AMB_RS02435 0.79 +0.4 505,085 - AMB_RS02435 0.79 +0.8 505,085 - AMB_RS02435 0.79 +0.2 505,087 + AMB_RS02435 0.79 +0.3 505,087 + AMB_RS02435 0.79 +0.4 505,087 + AMB_RS02435 0.79 -0.5 505,088 - AMB_RS02435 0.79 +0.8 505,088 - AMB_RS02435 0.79 -2.8 505,088 - AMB_RS02435 0.79 +1.8 505,183 + +1.0 505,183 + -0.5 505,183 + +0.1 505,184 - -0.6 505,184 - -0.0 505,184 - -2.4 505,254 + -0.8 505,254 + +0.8 505,291 - AMB_RS23135 0.12 -0.2 505,359 + AMB_RS23135 0.19 -1.0 505,530 + AMB_RS23135 0.38 -0.3 505,530 + AMB_RS23135 0.38 -0.6 505,530 + AMB_RS23135 0.38 -1.6 505,530 + AMB_RS23135 0.38 +0.5 505,530 + AMB_RS23135 0.38 -1.6 505,531 - AMB_RS23135 0.38 +0.3 505,531 - AMB_RS23135 0.38 -2.5 505,531 - AMB_RS23135 0.38 +0.0 505,696 - AMB_RS23135 0.57 -1.6 506,034 + +2.7 506,035 - -2.6 506,143 + AMB_RS02445 0.14 -2.0 506,143 + AMB_RS02445 0.14 -0.6 506,143 + AMB_RS02445 0.14 -0.4 506,144 - AMB_RS02445 0.14 -1.8
Or see this region's nucleotide sequence