Experiment: 150_an_nonmag_2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS23875 and AMB_RS02340 are separated by 130 nucleotides AMB_RS02340 and AMB_RS02345 overlap by 4 nucleotides AMB_RS02345 and AMB_RS02350 are separated by 4 nucleotides AMB_RS02350 and AMB_RS02355 are separated by 1 nucleotides AMB_RS02355 and AMB_RS02360 are separated by 65 nucleotides
AMB_RS23875: AMB_RS23875 - hypothetical protein, at 493,824 to 494,807
_RS23875
AMB_RS02340: AMB_RS02340 - nuclease inhibitor protein, at 494,938 to 495,474
_RS02340
AMB_RS02345: AMB_RS02345 - hypothetical protein, at 495,471 to 495,818
_RS02345
AMB_RS02350: AMB_RS02350 - hypothetical protein, at 495,823 to 496,104
_RS02350
AMB_RS02355: AMB_RS02355 - DUF1566 domain-containing protein, at 496,106 to 496,639
_RS02355
AMB_RS02360: AMB_RS02360 - DUF1566 domain-containing protein, at 496,705 to 497,247
_RS02360
Position (kb) 495
496 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 494.590 kb on + strand, within AMB_RS23875 at 495.167 kb on - strand, within AMB_RS02340 at 495.167 kb on - strand, within AMB_RS02340 at 495.167 kb on - strand, within AMB_RS02340 at 495.492 kb on + strand at 495.493 kb on - strand at 495.498 kb on + strand at 495.498 kb on + strand at 495.498 kb on + strand at 495.498 kb on + strand at 495.498 kb on + strand at 495.498 kb on + strand at 495.498 kb on + strand at 495.498 kb on + strand at 495.498 kb on + strand at 495.499 kb on - strand at 495.612 kb on + strand, within AMB_RS02345 at 495.612 kb on + strand, within AMB_RS02345 at 495.612 kb on + strand, within AMB_RS02345 at 495.612 kb on + strand, within AMB_RS02345 at 495.613 kb on - strand, within AMB_RS02345 at 495.804 kb on + strand at 495.804 kb on + strand at 495.805 kb on - strand at 495.805 kb on - strand at 496.151 kb on + strand at 496.151 kb on + strand at 496.152 kb on - strand at 496.166 kb on + strand, within AMB_RS02355 at 496.166 kb on + strand, within AMB_RS02355 at 496.166 kb on + strand, within AMB_RS02355 at 496.167 kb on - strand, within AMB_RS02355 at 496.199 kb on + strand, within AMB_RS02355 at 496.200 kb on - strand, within AMB_RS02355 at 496.200 kb on - strand, within AMB_RS02355 at 496.200 kb on - strand, within AMB_RS02355 at 496.386 kb on - strand, within AMB_RS02355 at 496.386 kb on - strand, within AMB_RS02355 at 496.386 kb on - strand, within AMB_RS02355 at 496.386 kb on - strand, within AMB_RS02355 at 496.445 kb on + strand, within AMB_RS02355 at 496.445 kb on + strand, within AMB_RS02355 at 496.445 kb on + strand, within AMB_RS02355 at 496.446 kb on - strand, within AMB_RS02355 at 496.446 kb on - strand, within AMB_RS02355 at 496.446 kb on - strand, within AMB_RS02355 at 496.446 kb on - strand, within AMB_RS02355 at 496.515 kb on - strand, within AMB_RS02355 at 496.515 kb on - strand, within AMB_RS02355 at 496.771 kb on + strand, within AMB_RS02360 at 496.772 kb on - strand, within AMB_RS02360 at 496.772 kb on - strand, within AMB_RS02360 at 496.786 kb on + strand, within AMB_RS02360 at 496.786 kb on + strand, within AMB_RS02360 at 496.786 kb on + strand, within AMB_RS02360 at 496.786 kb on + strand, within AMB_RS02360 at 496.786 kb on + strand, within AMB_RS02360 at 496.787 kb on - strand, within AMB_RS02360 at 496.787 kb on - strand, within AMB_RS02360 at 496.787 kb on - strand, within AMB_RS02360 at 496.787 kb on - strand, within AMB_RS02360 at 496.787 kb on - strand, within AMB_RS02360 at 496.787 kb on - strand, within AMB_RS02360 at 496.787 kb on - strand, within AMB_RS02360 at 496.795 kb on + strand, within AMB_RS02360 at 496.795 kb on + strand, within AMB_RS02360
Per-strain Table
Position Strand Gene LocusTag Fraction 150_an_nonmag_2 remove 494,590 + AMB_RS23875 0.78 -1.2 495,167 - AMB_RS02340 0.43 -1.0 495,167 - AMB_RS02340 0.43 -1.6 495,167 - AMB_RS02340 0.43 +0.2 495,492 + -0.5 495,493 - +0.4 495,498 + -0.8 495,498 + +0.2 495,498 + -0.8 495,498 + -0.9 495,498 + -0.2 495,498 + -0.8 495,498 + +0.8 495,498 + +0.4 495,498 + -0.3 495,499 - -1.4 495,612 + AMB_RS02345 0.41 +1.8 495,612 + AMB_RS02345 0.41 +0.8 495,612 + AMB_RS02345 0.41 +0.2 495,612 + AMB_RS02345 0.41 -4.1 495,613 - AMB_RS02345 0.41 -0.2 495,804 + -0.1 495,804 + -2.9 495,805 - -1.3 495,805 - +0.8 496,151 + -0.9 496,151 + -2.6 496,152 - -2.4 496,166 + AMB_RS02355 0.11 -0.2 496,166 + AMB_RS02355 0.11 -1.4 496,166 + AMB_RS02355 0.11 +0.5 496,167 - AMB_RS02355 0.11 -1.4 496,199 + AMB_RS02355 0.17 +0.2 496,200 - AMB_RS02355 0.18 -0.9 496,200 - AMB_RS02355 0.18 -0.2 496,200 - AMB_RS02355 0.18 +0.3 496,386 - AMB_RS02355 0.52 +0.1 496,386 - AMB_RS02355 0.52 +0.8 496,386 - AMB_RS02355 0.52 -1.9 496,386 - AMB_RS02355 0.52 -0.3 496,445 + AMB_RS02355 0.63 -1.7 496,445 + AMB_RS02355 0.63 -1.3 496,445 + AMB_RS02355 0.63 +0.2 496,446 - AMB_RS02355 0.64 -0.8 496,446 - AMB_RS02355 0.64 -0.2 496,446 - AMB_RS02355 0.64 -0.4 496,446 - AMB_RS02355 0.64 -0.5 496,515 - AMB_RS02355 0.77 -0.2 496,515 - AMB_RS02355 0.77 -1.2 496,771 + AMB_RS02360 0.12 +0.2 496,772 - AMB_RS02360 0.12 -2.9 496,772 - AMB_RS02360 0.12 -0.6 496,786 + AMB_RS02360 0.15 +0.8 496,786 + AMB_RS02360 0.15 -1.1 496,786 + AMB_RS02360 0.15 +0.0 496,786 + AMB_RS02360 0.15 -0.4 496,786 + AMB_RS02360 0.15 -3.1 496,787 - AMB_RS02360 0.15 +0.2 496,787 - AMB_RS02360 0.15 -0.2 496,787 - AMB_RS02360 0.15 -0.4 496,787 - AMB_RS02360 0.15 +0.5 496,787 - AMB_RS02360 0.15 -1.2 496,787 - AMB_RS02360 0.15 +0.2 496,787 - AMB_RS02360 0.15 +0.8 496,795 + AMB_RS02360 0.17 -3.7 496,795 + AMB_RS02360 0.17 -1.6
Or see this region's nucleotide sequence