Experiment: 150_an_nonmag_2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS23120 and AMB_RS02295 overlap by 16 nucleotides AMB_RS02295 and AMB_RS02300 are separated by 176 nucleotides AMB_RS02300 and AMB_RS02305 overlap by 8 nucleotides
AMB_RS23120: AMB_RS23120 - helix-turn-helix domain-containing protein, at 487,708 to 488,748
_RS23120
AMB_RS02295: AMB_RS02295 - XRE family transcriptional regulator, at 488,733 to 488,999
_RS02295
AMB_RS02300: AMB_RS02300 - hypothetical protein, at 489,176 to 489,676
_RS02300
AMB_RS02305: AMB_RS02305 - transposase, at 489,669 to 491,870
_RS02305
Position (kb) 488
489 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 487.737 kb on + strand at 487.738 kb on - strand at 488.423 kb on + strand, within AMB_RS23120 at 488.424 kb on - strand, within AMB_RS23120 at 488.427 kb on + strand, within AMB_RS23120 at 488.428 kb on - strand, within AMB_RS23120 at 488.428 kb on - strand, within AMB_RS23120 at 488.548 kb on - strand, within AMB_RS23120 at 488.548 kb on - strand, within AMB_RS23120 at 488.548 kb on - strand, within AMB_RS23120 at 488.781 kb on + strand, within AMB_RS02295 at 488.782 kb on - strand, within AMB_RS02295 at 488.967 kb on - strand, within AMB_RS02295 at 488.967 kb on - strand, within AMB_RS02295 at 489.017 kb on - strand at 489.829 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 150_an_nonmag_2 remove 487,737 + -0.9 487,738 - -1.0 488,423 + AMB_RS23120 0.69 -1.9 488,424 - AMB_RS23120 0.69 -0.1 488,427 + AMB_RS23120 0.69 -2.5 488,428 - AMB_RS23120 0.69 -1.8 488,428 - AMB_RS23120 0.69 -1.4 488,548 - AMB_RS23120 0.81 -0.8 488,548 - AMB_RS23120 0.81 -0.5 488,548 - AMB_RS23120 0.81 -0.1 488,781 + AMB_RS02295 0.18 +0.2 488,782 - AMB_RS02295 0.18 -0.2 488,967 - AMB_RS02295 0.88 -0.8 488,967 - AMB_RS02295 0.88 +0.3 489,017 - +0.4 489,829 - -3.7
Or see this region's nucleotide sequence