Experiment: 150_an_nonmag_2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS01115 and AMB_RS01120 are separated by 61 nucleotides AMB_RS01120 and AMB_RS01125 are separated by 339 nucleotides
AMB_RS01115: AMB_RS01115 - beta-glucosidase, at 247,901 to 249,262
_RS01115
AMB_RS01120: AMB_RS01120 - HAMP domain-containing protein, at 249,324 to 251,747
_RS01120
AMB_RS01125: AMB_RS01125 - phosphomethylpyrimidine synthase, at 252,087 to 253,928
_RS01125
Position (kb)
249
250
251
252 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 248.414 kb on + strand, within AMB_RS01115 at 248.414 kb on + strand, within AMB_RS01115 at 248.415 kb on - strand, within AMB_RS01115 at 248.415 kb on - strand, within AMB_RS01115 at 248.415 kb on - strand, within AMB_RS01115 at 248.415 kb on - strand, within AMB_RS01115 at 248.740 kb on + strand, within AMB_RS01115 at 248.801 kb on + strand, within AMB_RS01115 at 248.801 kb on + strand, within AMB_RS01115 at 248.801 kb on + strand, within AMB_RS01115 at 248.802 kb on - strand, within AMB_RS01115 at 248.804 kb on + strand, within AMB_RS01115 at 248.805 kb on - strand, within AMB_RS01115 at 248.918 kb on + strand, within AMB_RS01115 at 248.919 kb on - strand, within AMB_RS01115 at 248.990 kb on + strand, within AMB_RS01115 at 248.991 kb on - strand, within AMB_RS01115 at 249.046 kb on + strand, within AMB_RS01115 at 249.046 kb on + strand, within AMB_RS01115 at 249.046 kb on + strand, within AMB_RS01115 at 249.047 kb on - strand, within AMB_RS01115 at 249.146 kb on + strand at 249.146 kb on + strand at 249.146 kb on + strand at 249.147 kb on - strand at 249.196 kb on - strand at 249.196 kb on - strand at 249.202 kb on + strand at 249.203 kb on - strand at 249.203 kb on - strand at 249.218 kb on + strand at 249.218 kb on + strand at 249.218 kb on + strand at 249.218 kb on + strand at 249.219 kb on - strand at 249.219 kb on - strand at 249.219 kb on - strand at 249.219 kb on - strand at 249.219 kb on - strand at 249.219 kb on - strand at 249.219 kb on - strand at 249.219 kb on - strand at 249.306 kb on + strand at 249.306 kb on + strand at 249.306 kb on + strand at 249.383 kb on + strand at 249.523 kb on + strand at 250.568 kb on + strand, within AMB_RS01120 at 250.569 kb on - strand, within AMB_RS01120 at 250.569 kb on - strand, within AMB_RS01120 at 250.862 kb on + strand, within AMB_RS01120 at 250.863 kb on - strand, within AMB_RS01120 at 251.257 kb on + strand, within AMB_RS01120 at 251.257 kb on + strand, within AMB_RS01120 at 251.258 kb on - strand, within AMB_RS01120 at 251.395 kb on + strand, within AMB_RS01120 at 251.564 kb on + strand at 251.564 kb on + strand at 251.624 kb on - strand at 251.624 kb on - strand at 251.716 kb on + strand at 251.873 kb on - strand at 251.873 kb on - strand at 251.873 kb on - strand at 251.888 kb on + strand at 251.890 kb on + strand at 251.890 kb on + strand at 251.926 kb on + strand at 251.926 kb on + strand at 251.926 kb on + strand at 251.933 kb on + strand at 251.949 kb on - strand at 251.962 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 150_an_nonmag_2 remove 248,414 + AMB_RS01115 0.38 +1.9 248,414 + AMB_RS01115 0.38 -1.1 248,415 - AMB_RS01115 0.38 +0.5 248,415 - AMB_RS01115 0.38 -0.9 248,415 - AMB_RS01115 0.38 +0.3 248,415 - AMB_RS01115 0.38 +0.3 248,740 + AMB_RS01115 0.62 -1.1 248,801 + AMB_RS01115 0.66 +0.9 248,801 + AMB_RS01115 0.66 -0.4 248,801 + AMB_RS01115 0.66 -1.7 248,802 - AMB_RS01115 0.66 -0.4 248,804 + AMB_RS01115 0.66 -0.4 248,805 - AMB_RS01115 0.66 -0.8 248,918 + AMB_RS01115 0.75 -3.3 248,919 - AMB_RS01115 0.75 +0.6 248,990 + AMB_RS01115 0.80 -3.7 248,991 - AMB_RS01115 0.80 -0.4 249,046 + AMB_RS01115 0.84 -0.7 249,046 + AMB_RS01115 0.84 +0.9 249,046 + AMB_RS01115 0.84 -0.1 249,047 - AMB_RS01115 0.84 -0.2 249,146 + -0.7 249,146 + -0.7 249,146 + -0.7 249,147 - +0.7 249,196 - -0.6 249,196 - -0.7 249,202 + -0.5 249,203 - +0.3 249,203 - +0.6 249,218 + +0.9 249,218 + -0.2 249,218 + +0.1 249,218 + -2.0 249,219 - -3.2 249,219 - +0.9 249,219 - -2.0 249,219 - -4.8 249,219 - +1.9 249,219 - +1.0 249,219 - -0.3 249,219 - +0.7 249,306 + +0.2 249,306 + -0.9 249,306 + -0.8 249,383 + -0.6 249,523 + +1.9 250,568 + AMB_RS01120 0.51 -0.8 250,569 - AMB_RS01120 0.51 -2.6 250,569 - AMB_RS01120 0.51 +1.9 250,862 + AMB_RS01120 0.63 -0.5 250,863 - AMB_RS01120 0.63 -0.1 251,257 + AMB_RS01120 0.80 -0.4 251,257 + AMB_RS01120 0.80 -0.7 251,258 - AMB_RS01120 0.80 -0.9 251,395 + AMB_RS01120 0.85 -0.2 251,564 + +1.9 251,564 + -0.1 251,624 - -0.7 251,624 - +0.5 251,716 + +1.9 251,873 - -1.3 251,873 - -0.7 251,873 - -0.1 251,888 + +1.9 251,890 + -0.5 251,890 + +0.9 251,926 + +0.2 251,926 + -0.2 251,926 + -0.0 251,933 + -2.7 251,949 - -1.6 251,962 + +0.9
Or see this region's nucleotide sequence