Experiment: 150_an_nonmag_2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS00975 and AMB_RS00980 are separated by 85 nucleotides AMB_RS00980 and AMB_RS00985 are separated by 151 nucleotides AMB_RS00985 and AMB_RS00990 are separated by 64 nucleotides AMB_RS00990 and AMB_RS00995 are separated by 118 nucleotides
AMB_RS00975: AMB_RS00975 - hypothetical protein, at 220,033 to 220,494
_RS00975
AMB_RS00980: AMB_RS00980 - hypothetical protein, at 220,580 to 221,104
_RS00980
AMB_RS00985: AMB_RS00985 - D-glycerate dehydrogenase, at 221,256 to 222,242
_RS00985
AMB_RS00990: AMB_RS00990 - hypothetical protein, at 222,307 to 222,831
_RS00990
AMB_RS00995: AMB_RS00995 - hypothetical protein, at 222,950 to 223,396
_RS00995
Position (kb) 221
222
223 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 220.480 kb on + strand at 220.480 kb on + strand at 220.480 kb on + strand at 220.480 kb on + strand at 220.480 kb on + strand at 220.480 kb on + strand at 220.582 kb on - strand at 221.069 kb on - strand at 221.069 kb on - strand at 221.105 kb on - strand at 221.105 kb on - strand at 221.105 kb on - strand at 221.122 kb on - strand at 221.124 kb on + strand at 221.125 kb on - strand at 221.127 kb on - strand at 221.174 kb on + strand at 221.174 kb on + strand at 221.174 kb on + strand at 221.175 kb on - strand at 221.219 kb on + strand at 221.220 kb on - strand at 221.220 kb on - strand at 221.220 kb on - strand at 221.220 kb on - strand at 221.787 kb on + strand, within AMB_RS00985 at 221.787 kb on + strand, within AMB_RS00985 at 221.787 kb on + strand, within AMB_RS00985 at 221.787 kb on + strand, within AMB_RS00985 at 221.842 kb on - strand, within AMB_RS00985 at 221.842 kb on - strand, within AMB_RS00985 at 221.842 kb on - strand, within AMB_RS00985 at 221.842 kb on - strand, within AMB_RS00985 at 221.903 kb on + strand, within AMB_RS00985 at 221.964 kb on + strand, within AMB_RS00985 at 221.964 kb on + strand, within AMB_RS00985 at 221.964 kb on + strand, within AMB_RS00985 at 221.964 kb on + strand, within AMB_RS00985 at 221.964 kb on + strand, within AMB_RS00985 at 221.964 kb on + strand, within AMB_RS00985 at 221.964 kb on + strand, within AMB_RS00985 at 221.965 kb on - strand, within AMB_RS00985 at 221.965 kb on - strand, within AMB_RS00985 at 221.965 kb on - strand, within AMB_RS00985 at 221.965 kb on - strand, within AMB_RS00985 at 221.965 kb on - strand, within AMB_RS00985 at 222.240 kb on + strand at 222.240 kb on + strand at 222.240 kb on + strand at 222.241 kb on - strand at 222.241 kb on - strand at 222.241 kb on - strand at 222.241 kb on - strand at 222.241 kb on - strand at 222.247 kb on + strand at 222.434 kb on + strand, within AMB_RS00990 at 222.434 kb on + strand, within AMB_RS00990 at 222.434 kb on + strand, within AMB_RS00990 at 222.434 kb on + strand, within AMB_RS00990 at 222.435 kb on - strand, within AMB_RS00990 at 222.435 kb on - strand, within AMB_RS00990 at 222.435 kb on - strand, within AMB_RS00990 at 222.435 kb on - strand, within AMB_RS00990
Per-strain Table
Position Strand Gene LocusTag Fraction 150_an_nonmag_2 remove 220,480 + -1.6 220,480 + -2.0 220,480 + -0.7 220,480 + -1.9 220,480 + -1.7 220,480 + -2.2 220,582 - +0.1 221,069 - -0.1 221,069 - -0.8 221,105 - +0.9 221,105 - +0.3 221,105 - +0.8 221,122 - -0.1 221,124 + -1.7 221,125 - -2.1 221,127 - -2.3 221,174 + -0.8 221,174 + -1.9 221,174 + -3.0 221,175 - +1.9 221,219 + -0.7 221,220 - +0.3 221,220 - -1.4 221,220 - +0.9 221,220 - -1.1 221,787 + AMB_RS00985 0.54 -0.1 221,787 + AMB_RS00985 0.54 -1.9 221,787 + AMB_RS00985 0.54 -3.3 221,787 + AMB_RS00985 0.54 -1.3 221,842 - AMB_RS00985 0.59 -1.4 221,842 - AMB_RS00985 0.59 -0.9 221,842 - AMB_RS00985 0.59 -0.7 221,842 - AMB_RS00985 0.59 -0.9 221,903 + AMB_RS00985 0.66 -2.6 221,964 + AMB_RS00985 0.72 +0.0 221,964 + AMB_RS00985 0.72 +0.3 221,964 + AMB_RS00985 0.72 -0.8 221,964 + AMB_RS00985 0.72 -1.9 221,964 + AMB_RS00985 0.72 -1.3 221,964 + AMB_RS00985 0.72 -0.7 221,964 + AMB_RS00985 0.72 -1.3 221,965 - AMB_RS00985 0.72 -0.4 221,965 - AMB_RS00985 0.72 -1.2 221,965 - AMB_RS00985 0.72 -1.9 221,965 - AMB_RS00985 0.72 +1.2 221,965 - AMB_RS00985 0.72 -0.7 222,240 + -0.4 222,240 + -0.4 222,240 + -0.1 222,241 - -0.4 222,241 - +0.1 222,241 - +0.9 222,241 - -2.4 222,241 - -0.1 222,247 + -0.4 222,434 + AMB_RS00990 0.24 -0.1 222,434 + AMB_RS00990 0.24 +1.9 222,434 + AMB_RS00990 0.24 +0.3 222,434 + AMB_RS00990 0.24 -0.9 222,435 - AMB_RS00990 0.24 -1.8 222,435 - AMB_RS00990 0.24 -0.7 222,435 - AMB_RS00990 0.24 -2.8 222,435 - AMB_RS00990 0.24 -0.9
Or see this region's nucleotide sequence