Experiment: 150_an_nonmag_2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS00410 and AMB_RS00415 are separated by 3 nucleotides AMB_RS00415 and AMB_RS00420 overlap by 76 nucleotides
AMB_RS00410: AMB_RS00410 - class I SAM-dependent methyltransferase, at 89,363 to 90,535
_RS00410
AMB_RS00415: AMB_RS00415 - histidinol-phosphatase, at 90,539 to 91,252
_RS00415
AMB_RS00420: AMB_RS00420 - hypothetical protein, at 91,177 to 92,289
_RS00420
Position (kb) 90
91
92 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 89.564 kb on + strand, within AMB_RS00410 at 89.564 kb on + strand, within AMB_RS00410 at 89.564 kb on + strand, within AMB_RS00410 at 89.579 kb on + strand, within AMB_RS00410 at 89.580 kb on - strand, within AMB_RS00410 at 89.580 kb on - strand, within AMB_RS00410 at 89.580 kb on - strand, within AMB_RS00410 at 89.714 kb on + strand, within AMB_RS00410 at 89.714 kb on + strand, within AMB_RS00410 at 89.714 kb on + strand, within AMB_RS00410 at 89.714 kb on + strand, within AMB_RS00410 at 89.714 kb on + strand, within AMB_RS00410 at 89.714 kb on + strand, within AMB_RS00410 at 89.714 kb on + strand, within AMB_RS00410 at 89.714 kb on + strand, within AMB_RS00410 at 89.714 kb on + strand, within AMB_RS00410 at 89.714 kb on + strand, within AMB_RS00410 at 89.714 kb on + strand, within AMB_RS00410 at 89.715 kb on - strand, within AMB_RS00410 at 89.715 kb on - strand, within AMB_RS00410 at 89.715 kb on - strand, within AMB_RS00410 at 89.715 kb on - strand, within AMB_RS00410 at 89.715 kb on - strand, within AMB_RS00410 at 89.715 kb on - strand, within AMB_RS00410 at 89.730 kb on + strand, within AMB_RS00410 at 89.759 kb on + strand, within AMB_RS00410 at 89.759 kb on + strand, within AMB_RS00410 at 89.759 kb on + strand, within AMB_RS00410 at 89.760 kb on - strand, within AMB_RS00410 at 89.760 kb on - strand, within AMB_RS00410 at 89.819 kb on + strand, within AMB_RS00410 at 89.819 kb on + strand, within AMB_RS00410 at 89.819 kb on + strand, within AMB_RS00410 at 89.819 kb on + strand, within AMB_RS00410 at 89.960 kb on + strand, within AMB_RS00410 at 89.961 kb on - strand, within AMB_RS00410 at 89.961 kb on - strand, within AMB_RS00410 at 90.027 kb on - strand, within AMB_RS00410 at 90.027 kb on - strand, within AMB_RS00410 at 90.289 kb on + strand, within AMB_RS00410 at 90.419 kb on + strand at 90.420 kb on - strand at 90.440 kb on + strand at 90.440 kb on + strand at 90.440 kb on + strand at 90.440 kb on + strand at 90.440 kb on + strand at 90.440 kb on + strand at 90.440 kb on + strand at 90.440 kb on + strand at 90.440 kb on + strand at 90.440 kb on + strand at 90.440 kb on + strand at 90.440 kb on + strand at 90.440 kb on + strand at 90.440 kb on + strand at 90.441 kb on - strand at 90.928 kb on + strand, within AMB_RS00415
Per-strain Table
Position Strand Gene LocusTag Fraction 150_an_nonmag_2 remove 89,564 + AMB_RS00410 0.17 -0.2 89,564 + AMB_RS00410 0.17 -0.5 89,564 + AMB_RS00410 0.17 -0.1 89,579 + AMB_RS00410 0.18 -0.2 89,580 - AMB_RS00410 0.18 -3.2 89,580 - AMB_RS00410 0.18 -0.0 89,580 - AMB_RS00410 0.18 -1.4 89,714 + AMB_RS00410 0.30 -0.7 89,714 + AMB_RS00410 0.30 -1.3 89,714 + AMB_RS00410 0.30 -0.2 89,714 + AMB_RS00410 0.30 -1.5 89,714 + AMB_RS00410 0.30 -0.0 89,714 + AMB_RS00410 0.30 -1.1 89,714 + AMB_RS00410 0.30 -0.1 89,714 + AMB_RS00410 0.30 -1.4 89,714 + AMB_RS00410 0.30 -0.3 89,714 + AMB_RS00410 0.30 -0.1 89,714 + AMB_RS00410 0.30 +0.9 89,715 - AMB_RS00410 0.30 +0.3 89,715 - AMB_RS00410 0.30 -1.4 89,715 - AMB_RS00410 0.30 -0.6 89,715 - AMB_RS00410 0.30 +0.9 89,715 - AMB_RS00410 0.30 -4.2 89,715 - AMB_RS00410 0.30 -2.1 89,730 + AMB_RS00410 0.31 +0.6 89,759 + AMB_RS00410 0.34 -0.8 89,759 + AMB_RS00410 0.34 +0.4 89,759 + AMB_RS00410 0.34 -0.7 89,760 - AMB_RS00410 0.34 -3.5 89,760 - AMB_RS00410 0.34 -1.8 89,819 + AMB_RS00410 0.39 -0.5 89,819 + AMB_RS00410 0.39 -0.1 89,819 + AMB_RS00410 0.39 +0.3 89,819 + AMB_RS00410 0.39 -0.6 89,960 + AMB_RS00410 0.51 -1.3 89,961 - AMB_RS00410 0.51 -2.9 89,961 - AMB_RS00410 0.51 -0.1 90,027 - AMB_RS00410 0.57 -1.1 90,027 - AMB_RS00410 0.57 +0.3 90,289 + AMB_RS00410 0.79 -2.6 90,419 + +0.3 90,420 - -0.4 90,440 + -0.1 90,440 + +0.5 90,440 + +0.2 90,440 + +0.7 90,440 + +0.3 90,440 + -0.4 90,440 + +0.3 90,440 + -0.5 90,440 + -0.4 90,440 + -0.1 90,440 + +0.1 90,440 + -1.4 90,440 + -0.4 90,440 + +1.3 90,441 - -3.1 90,928 + AMB_RS00415 0.54 -1.3
Or see this region's nucleotide sequence