Strain Fitness in Escherichia coli BW25113 around b3894

Experiment: Spectinomycin 0.00625 mg/ml

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	Spectinomycin 0.00625 mg/ml
4,079,818	-	fdoI	b3892	0.90	+2.6
4,079,832	-				+2.2
4,079,903	-				+1.5
4,079,903	-				+1.9
4,079,930	-				+1.3
4,079,930	-				+1.2
4,079,937	+				+1.3
4,079,945	-				+2.0
4,079,945	-				+1.5
4,079,945	-				+1.1
4,079,945	-				+2.4
4,079,960	-				+1.9
4,079,965	+				+2.1
4,079,999	-	fdoH	b3893	0.13	+0.5
4,079,999	-	fdoH	b3893	0.13	+1.5
4,080,077	-	fdoH	b3893	0.22	+1.5
4,080,096	+	fdoH	b3893	0.24	+1.7
4,080,096	+	fdoH	b3893	0.24	+1.7
4,080,096	+	fdoH	b3893	0.24	+1.5
4,080,104	+	fdoH	b3893	0.25	+1.5
4,080,104	-	fdoH	b3893	0.25	+2.1
4,080,105	-	fdoH	b3893	0.25	+0.7
4,080,121	-	fdoH	b3893	0.27	+1.9
4,080,138	+	fdoH	b3893	0.29	+1.4
4,080,141	+	fdoH	b3893	0.29	+1.2
4,080,165	-	fdoH	b3893	0.32	+1.6
4,080,169	+	fdoH	b3893	0.32	+4.3
4,080,278	+	fdoH	b3893	0.44	+1.5
4,080,288	+	fdoH	b3893	0.45	+1.3
4,080,299	+	fdoH	b3893	0.46	+2.6
4,080,299	+	fdoH	b3893	0.46	+1.2
4,080,345	+	fdoH	b3893	0.51	+1.2
4,080,394	+	fdoH	b3893	0.57	+1.5
4,080,395	-	fdoH	b3893	0.57	+1.7
4,080,410	-	fdoH	b3893	0.59	+1.5
4,080,415	+	fdoH	b3893	0.59	+1.5
4,080,415	+	fdoH	b3893	0.59	+0.9
4,080,460	-	fdoH	b3893	0.64	+1.5
4,080,460	-	fdoH	b3893	0.64	+2.1
4,080,462	+	fdoH	b3893	0.64	+1.9
4,080,480	-	fdoH	b3893	0.66	+1.1
4,080,486	-	fdoH	b3893	0.67	+1.5
4,080,486	-	fdoH	b3893	0.67	+2.5
4,080,540	+	fdoH	b3893	0.73	+1.9
4,080,548	-	fdoH	b3893	0.74	+2.0
4,080,571	+	fdoH	b3893	0.77	+0.8
4,080,582	+	fdoH	b3893	0.78	+1.5
4,080,582	+	fdoH	b3893	0.78	+0.9
4,080,598	-	fdoH	b3893	0.80	+2.7
4,080,598	-	fdoH	b3893	0.80	+1.5
4,080,598	-	fdoH	b3893	0.80	+1.4
4,080,608	+	fdoH	b3893	0.81	+1.8
4,080,632	-	fdoH	b3893	0.83	+1.2
4,080,649	-	fdoH	b3893	0.85	+1.5
4,080,653	+	fdoH	b3893	0.86	+0.5
4,080,653	+	fdoH	b3893	0.86	+0.8
4,080,670	+	fdoH	b3893	0.87	+1.6
4,080,670	+	fdoH	b3893	0.87	+0.0
4,080,707	-				+1.3
4,080,707	-				+2.4
4,080,786	-				+2.0
4,080,786	-				-1.1
4,080,792	+				+0.7
4,080,831	-				+2.5
4,080,842	+				+1.8
4,080,864	-				+2.3
4,080,904	+				+3.0
4,080,926	+				+0.5
4,081,022	-				+1.7
4,081,022	-				+2.3
4,081,025	+				+2.5
4,081,038	+				+2.1
4,081,038	+				+1.1
4,081,041	-				+1.5
4,081,041	-				+0.9
4,081,047	-				+1.7
4,081,123	+	fdoG	b3894	0.11	+2.3
4,081,123	+	fdoG	b3894	0.11	+2.9
4,081,189	+	fdoG	b3894	0.13	+2.0
4,081,189	+	fdoG	b3894	0.13	+2.1
4,081,189	+	fdoG	b3894	0.13	+0.9
4,081,194	+	fdoG	b3894	0.13	+1.1
4,081,315	+	fdoG	b3894	0.17	+1.6
4,081,315	+	fdoG	b3894	0.17	+0.9
4,081,321	+	fdoG	b3894	0.17	+2.2
4,081,323	+	fdoG	b3894	0.17	+1.7
4,081,416	+	fdoG	b3894	0.20	+2.1
4,081,438	-	fdoG	b3894	0.21	+0.5
4,081,443	-	fdoG	b3894	0.21	+1.5
4,081,443	-	fdoG	b3894	0.21	+2.0
4,081,468	+	fdoG	b3894	0.22	+1.6
4,081,482	-	fdoG	b3894	0.23	+1.8
4,081,537	-	fdoG	b3894	0.24	+2.1
4,081,545	+	fdoG	b3894	0.25	+0.6
4,081,576	+	fdoG	b3894	0.26	+2.8
4,081,612	-	fdoG	b3894	0.27	+0.7
4,081,612	-	fdoG	b3894	0.27	-1.1
4,081,620	-	fdoG	b3894	0.27	+2.2
4,081,646	-	fdoG	b3894	0.28	+1.7
4,081,652	-	fdoG	b3894	0.28	+2.0
4,081,654	-	fdoG	b3894	0.28	+3.4
4,081,654	-	fdoG	b3894	0.28	+1.4
4,081,687	-	fdoG	b3894	0.29	+0.7
4,081,753	-	fdoG	b3894	0.31	+2.3
4,081,753	-	fdoG	b3894	0.31	+1.9
4,081,810	+	fdoG	b3894	0.33	+1.9
4,081,827	+	fdoG	b3894	0.34	-0.0
4,081,837	+	fdoG	b3894	0.34	+0.7
4,081,837	+	fdoG	b3894	0.34	+1.3
4,081,855	-	fdoG	b3894	0.35	+2.0
4,081,859	+	fdoG	b3894	0.35	+2.1
4,081,859	+	fdoG	b3894	0.35	+1.8
4,081,877	+	fdoG	b3894	0.35	+1.6
4,081,923	+	fdoG	b3894	0.37	+2.0
4,081,932	+	fdoG	b3894	0.37	+1.9
4,081,932	+	fdoG	b3894	0.37	+2.0
4,081,938	-	fdoG	b3894	0.37	-0.5
4,081,938	-	fdoG	b3894	0.37	+2.5
4,081,951	-	fdoG	b3894	0.38	+1.9
4,081,975	-	fdoG	b3894	0.39	+1.5
4,081,975	-	fdoG	b3894	0.39	+1.7
4,082,003	-	fdoG	b3894	0.40	+2.2
4,082,003	-	fdoG	b3894	0.40	+0.9
4,082,052	-	fdoG	b3894	0.41	+1.2
4,082,104	+	fdoG	b3894	0.43	+1.7
4,082,134	-	fdoG	b3894	0.44	+1.8
4,082,157	+	fdoG	b3894	0.45	+2.7
4,082,157	+	fdoG	b3894	0.45	+0.7
4,082,208	-	fdoG	b3894	0.46	+2.5
4,082,208	-	fdoG	b3894	0.46	+2.2
4,082,234	-	fdoG	b3894	0.47	+1.2
4,082,238	+	fdoG	b3894	0.47	+1.3
4,082,276	-	fdoG	b3894	0.49	+1.4
4,082,276	-	fdoG	b3894	0.49	+2.4
4,082,276	-	fdoG	b3894	0.49	+1.5
4,082,318	+	fdoG	b3894	0.50	+1.5
4,082,327	-	fdoG	b3894	0.50	+2.2
4,082,353	+	fdoG	b3894	0.51	+1.5
4,082,446	-	fdoG	b3894	0.54	+1.2
4,082,523	-	fdoG	b3894	0.57	+1.6
4,082,523	-	fdoG	b3894	0.57	+2.2
4,082,540	+	fdoG	b3894	0.57	+1.6
4,082,603	+	fdoG	b3894	0.59	+0.3
4,082,608	+	fdoG	b3894	0.59	+1.8
4,082,614	-	fdoG	b3894	0.60	+1.3
4,082,622	+	fdoG	b3894	0.60	+1.4
4,082,622	+	fdoG	b3894	0.60	+2.7
4,082,625	+	fdoG	b3894	0.60	+1.6
4,082,626	-	fdoG	b3894	0.60	+2.1
4,082,626	-	fdoG	b3894	0.60	+1.5
4,082,630	-	fdoG	b3894	0.60	+1.5
4,082,630	-	fdoG	b3894	0.60	+3.6
4,082,688	-	fdoG	b3894	0.62	+1.1
4,082,726	+	fdoG	b3894	0.63	+1.0
4,082,731	-	fdoG	b3894	0.63	+1.1
4,082,768	-	fdoG	b3894	0.65	+1.1
4,082,794	-	fdoG	b3894	0.66	+2.7
4,082,838	-				+1.9
4,082,838	-	fdoG	b3894	0.67	+1.2
4,082,842	+	fdoG	b3894	0.67	+0.4
4,082,842	+	fdoG	b3894	0.67	+1.3
4,082,895	+	fdoG	b3894	0.69	+1.7
4,082,895	+	fdoG	b3894	0.69	+2.2
4,082,903	-	fdoG	b3894	0.69	+1.8
4,082,918	+	fdoG	b3894	0.70	+2.9
4,082,941	+	fdoG	b3894	0.70	+1.5
4,082,941	+	fdoG	b3894	0.70	+1.2
4,082,967	+	fdoG	b3894	0.71	+1.5
4,082,967	+	fdoG	b3894	0.71	+2.7
4,082,967	+	fdoG	b3894	0.71	+1.9
4,082,984	-	fdoG	b3894	0.72	+3.2
4,082,984	-	fdoG	b3894	0.72	+1.7
4,082,994	-	fdoG	b3894	0.72	+1.6
4,083,027	-	fdoG	b3894	0.73	+2.6
4,083,028	+	fdoG	b3894	0.73	+1.9
4,083,028	+	fdoG	b3894	0.73	+2.3
4,083,030	+	fdoG	b3894	0.73	+2.1
4,083,161	+	fdoG	b3894	0.78	+2.1
4,083,212	-	fdoG	b3894	0.79	+1.3
4,083,245	+	fdoG	b3894	0.80	+1.6
4,083,245	-	fdoG	b3894	0.80	+1.3
4,083,273	+	fdoG	b3894	0.81	+0.8
4,083,286	+	fdoG	b3894	0.82	+1.0
4,083,286	+	fdoG	b3894	0.82	+2.1
4,083,378	-	fdoG	b3894	0.85	+1.5
4,083,378	-	fdoG	b3894	0.85	+2.4
4,083,383	-	fdoG	b3894	0.85	+0.9
4,083,421	-	fdoG	b3894	0.86	+1.3
4,083,421	-	fdoG	b3894	0.86	+1.6
4,083,467	-	fdoG	b3894	0.88	+1.2
4,083,495	+	fdoG	b3894	0.88	+1.9
4,083,557	-				+1.2
4,083,585	+				+2.7
4,083,585	+				+3.1
4,083,658	-				+1.9
4,083,671	+				+2.1
4,083,671	+				+2.0
4,083,671	+				+2.6
4,083,671	+				+2.2
4,083,735	-				+2.5
4,083,792	-				+1.8
4,083,792	-				+2.1
4,083,832	+				+2.4
4,083,832	+				+1.3
4,083,856	-				-0.2
4,083,856	-				+2.1
4,083,874	-				-1.0
4,083,874	-				+0.0
4,083,896	-				-1.1
4,083,896	-				+1.0
4,083,915	-				+1.8
4,083,921	-				-0.4
4,083,921	-				-1.4
4,083,943	-				+0.9
4,083,943	-				-1.4
4,083,980	-				-0.3
4,084,005	+				-0.3
4,084,005	+				-0.8
4,084,009	+				+0.1
4,084,017	+				+1.4
4,084,023	+				+1.5
4,084,023	+				+1.1
4,084,135	+	fdhD	b3895	0.12	+2.1
4,084,139	+	fdhD	b3895	0.12	+1.6
4,084,197	+	fdhD	b3895	0.19	+2.4
4,084,206	-	fdhD	b3895	0.20	+2.1
4,084,209	+	fdhD	b3895	0.20	+1.3
4,084,261	-	fdhD	b3895	0.27	+2.6
4,084,316	-	fdhD	b3895	0.33	+1.8
4,084,316	-	fdhD	b3895	0.33	+0.9
4,084,366	-	fdhD	b3895	0.39	+1.6
4,084,410	-	fdhD	b3895	0.44	+1.4
4,084,410	-	fdhD	b3895	0.44	+1.6
4,084,455	+	fdhD	b3895	0.50	+2.2
4,084,455	+	fdhD	b3895	0.50	+0.2
4,084,469	+	fdhD	b3895	0.52	+0.8
4,084,541	+	fdhD	b3895	0.60	+2.3
4,084,541	+	fdhD	b3895	0.60	+2.0
4,084,549	-	fdhD	b3895	0.61	+2.2
4,084,549	-	fdhD	b3895	0.61	+3.2
4,084,626	-	fdhD	b3895	0.70	+1.3
4,084,648	+	fdhD	b3895	0.73	+0.6
4,084,648	+	fdhD	b3895	0.73	+2.1
4,084,661	+	fdhD	b3895	0.75	+2.1
4,084,673	+	fdhD	b3895	0.76	+2.3
4,084,728	+	fdhD	b3895	0.83	+0.7
4,084,758	+	fdhD	b3895	0.86	+1.4
4,084,758	+	fdhD	b3895	0.86	+1.8
4,084,771	+	fdhD	b3895	0.88	+0.8
4,084,774	+	fdhD	b3895	0.88	+1.7
4,084,775	+	fdhD	b3895	0.88	+2.1
4,084,775	+	fdhD	b3895	0.88	+2.5
4,084,775	+	fdhD	b3895	0.88	+2.0
4,084,781	+	fdhD	b3895	0.89	+2.0
4,084,786	+	fdhD	b3895	0.90	+0.2
4,084,794	+				+1.9
4,084,794	+				+1.7
4,084,794	+				+1.8
4,084,797	+				+1.6
4,084,799	+				+2.5
4,084,799	+				+1.3
4,084,799	+				+1.9
4,084,799	+				+2.7
4,084,799	+				+0.4
4,084,799	+				+1.3
4,084,807	+				+2.1
4,084,817	+				+1.5
4,084,827	+				+1.8
4,084,833	+				+2.1
4,084,833	+				+1.4
4,084,838	+				+1.5
4,084,838	+				+1.9
4,084,839	+				+2.0
4,084,839	+				+1.5
4,084,839	+				+1.6
4,084,839	+				+0.7
4,084,839	+				+1.6
4,084,839	+				+2.5
4,084,839	+				+0.5
4,084,840	+				+0.9
4,084,840	+				+1.5
4,084,840	+				+0.9
4,084,840	+				+2.4
4,084,840	+				+2.9
4,084,840	+				+2.9
4,084,840	+				+2.2
4,084,840	+				+2.0
4,084,840	+				+1.7
4,084,840	+				+1.5
4,084,840	+				+1.8
4,084,840	+				+1.9
4,084,840	+				+1.1
4,084,844	+				+2.4
4,084,844	+				+1.2