Experiment: Spectinomycin 0.00625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yggN and yggL are separated by 183 nucleotides yggL and yggH overlap by 1 nucleotides yggH and mutY are separated by 160 nucleotides
b2958: yggN - hypothetical protein (NCBI), at 3,098,926 to 3,099,645
yggN
b2959: yggL - orf, hypothetical protein (VIMSS), at 3,099,829 to 3,100,155
yggL
b2960: yggH - tRNA(m7G46)-methyltransferase (NCBI), at 3,100,155 to 3,100,874
yggH
b2961: mutY - adenine DNA glycosylase (NCBI), at 3,101,035 to 3,102,087
mutY
Position (kb)
3099
3100
3101 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3099.052 kb on + strand, within yggN at 3099.062 kb on + strand, within yggN at 3099.190 kb on + strand, within yggN at 3099.216 kb on + strand, within yggN at 3099.216 kb on + strand, within yggN at 3099.347 kb on + strand, within yggN at 3099.350 kb on - strand, within yggN at 3099.350 kb on - strand, within yggN at 3099.366 kb on + strand, within yggN at 3099.366 kb on + strand, within yggN at 3099.482 kb on - strand, within yggN at 3099.484 kb on - strand, within yggN at 3099.533 kb on + strand, within yggN at 3099.564 kb on + strand, within yggN at 3099.564 kb on + strand, within yggN at 3099.564 kb on + strand, within yggN at 3099.569 kb on + strand, within yggN at 3099.868 kb on - strand, within yggL at 3099.907 kb on + strand, within yggL at 3099.928 kb on - strand, within yggL at 3100.069 kb on - strand, within yggL at 3100.069 kb on - strand, within yggL at 3100.096 kb on + strand, within yggL at 3100.181 kb on + strand at 3100.187 kb on - strand at 3100.187 kb on - strand at 3100.189 kb on - strand at 3100.189 kb on - strand at 3100.250 kb on + strand, within yggH at 3100.250 kb on + strand, within yggH at 3100.258 kb on - strand, within yggH at 3100.258 kb on - strand, within yggH at 3100.258 kb on - strand, within yggH at 3100.260 kb on - strand, within yggH at 3100.393 kb on - strand, within yggH at 3100.406 kb on - strand, within yggH at 3100.441 kb on + strand, within yggH at 3100.441 kb on + strand, within yggH at 3100.474 kb on + strand, within yggH at 3100.521 kb on - strand, within yggH at 3100.521 kb on - strand, within yggH at 3100.568 kb on - strand, within yggH at 3100.600 kb on + strand, within yggH at 3100.600 kb on + strand, within yggH at 3100.616 kb on - strand, within yggH at 3100.827 kb on - strand at 3100.829 kb on - strand at 3100.974 kb on + strand at 3100.974 kb on + strand at 3100.974 kb on + strand at 3101.012 kb on - strand at 3101.070 kb on - strand at 3101.070 kb on - strand at 3101.122 kb on + strand at 3101.122 kb on + strand at 3101.124 kb on + strand at 3101.124 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.00625 mg/ml remove 3,099,052 + yggN b2958 0.17 +0.4 3,099,062 + yggN b2958 0.19 -1.6 3,099,190 + yggN b2958 0.37 +0.5 3,099,216 + yggN b2958 0.40 -0.2 3,099,216 + yggN b2958 0.40 -0.0 3,099,347 + yggN b2958 0.58 +1.0 3,099,350 - yggN b2958 0.59 +0.6 3,099,350 - yggN b2958 0.59 -0.4 3,099,366 + yggN b2958 0.61 +0.5 3,099,366 + yggN b2958 0.61 +1.6 3,099,482 - yggN b2958 0.77 -0.1 3,099,484 - yggN b2958 0.78 +0.4 3,099,533 + yggN b2958 0.84 +0.6 3,099,564 + yggN b2958 0.89 +0.5 3,099,564 + yggN b2958 0.89 -0.2 3,099,564 + yggN b2958 0.89 -0.6 3,099,569 + yggN b2958 0.89 +0.2 3,099,868 - yggL b2959 0.12 -0.4 3,099,907 + yggL b2959 0.24 -0.6 3,099,928 - yggL b2959 0.30 -0.2 3,100,069 - yggL b2959 0.73 -0.2 3,100,069 - yggL b2959 0.73 -2.3 3,100,096 + yggL b2959 0.82 -0.8 3,100,181 + -0.0 3,100,187 - +0.3 3,100,187 - -0.4 3,100,189 - -0.9 3,100,189 - -1.2 3,100,250 + yggH b2960 0.13 +1.8 3,100,250 + yggH b2960 0.13 +2.4 3,100,258 - yggH b2960 0.14 +1.2 3,100,258 - yggH b2960 0.14 -0.2 3,100,258 - yggH b2960 0.14 +1.1 3,100,260 - yggH b2960 0.15 +0.8 3,100,393 - yggH b2960 0.33 -0.8 3,100,406 - yggH b2960 0.35 -0.0 3,100,441 + yggH b2960 0.40 +1.5 3,100,441 + yggH b2960 0.40 +1.2 3,100,474 + yggH b2960 0.44 +0.4 3,100,521 - yggH b2960 0.51 +0.8 3,100,521 - yggH b2960 0.51 +0.2 3,100,568 - yggH b2960 0.57 +0.2 3,100,600 + yggH b2960 0.62 +1.1 3,100,600 + yggH b2960 0.62 +1.4 3,100,616 - yggH b2960 0.64 +1.2 3,100,827 - -0.4 3,100,829 - +1.0 3,100,974 + +0.5 3,100,974 + +0.2 3,100,974 + +1.2 3,101,012 - -0.1 3,101,070 - +0.2 3,101,070 - +1.2 3,101,122 + +2.0 3,101,122 + +0.6 3,101,124 + -0.3 3,101,124 + +0.1
Or see this region's nucleotide sequence